Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

Costa, Larissa Kelly Cunha; Siqueira, Jones Anderson Monteiro; Portal, Thayara Morais; Júnior, Edivaldo Costa Sousa; Linhares, Alexandre da Costa; Gabbay, Yvone Benchimol; Resque, Hugo Reis

doi:10.1590/0037-8682-0198-2017

Cited by 15 publications

(4 citation statements)

References 38 publications

(44 reference statements)

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“…The occurrence of SV in outpatients and hospitalised settings has been reported with different rates in various developing countries [3]. In this study, the prevalence rate (14.1%) is high compared to a 7.7% SV detection rate previously reported in SA from hospitalised patients [27], 5.2% [28], and 2.5% [29] reported in Brazil. However comparable detection rate (13.9%) was observed in hospitalized patients from Pakistan [30].…”

Section: Discussionmentioning

confidence: 44%

Prevalence and Genetic Characterisation of Human Sapovirus from Children with Diarrhoea in the Rural Areas of Vhembe District, South Africa, 2017–2020

Magwalivha

Ngandu

Traore

et al. 2021

Viruses

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Diarrhoeal disease is considered an important cause of morbidity and mortality in developing areas, and a large contributor to the burden of disease in children younger than five years of age. This study investigated the prevalence and genogroups of human sapovirus (SV) in children ≤5 years of age in rural communities of Vhembe district, South Africa. Between 2017 and 2020, a total of 284 stool samples were collected from children suffering with diarrhoea (n = 228) and from children without diarrhoea (n = 56). RNA extraction using Boom extraction method, and screening for SV using real-time PCR were done in the lab. Positive samples were subjected to conventional RT-PCR targeting the capsid fragment. Positive sample isolates were genotyped using Sanger sequencing. Overall SV were detected in 14.1% (40/284) of the stool samples (16.7% (38/228) of diarrhoeal and 3.6% (2/56) of non-diarrhoeal samples). Significant correlation between SV positive cases and water sources was noted. Genogroup-I was identified as the most prevalent strain comprising 81.3% (13/16), followed by SV-GII 12.5% (2/16) and SV-GIV 6.2% (1/16). This study provides valuable data on prevalence of SV amongst outpatients in rural and underdeveloped communities, and highlights the necessity for further monitoring of SV circulating strains as potential emerging strains.

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Section: Discussionmentioning

confidence: 44%

Prevalence and Genetic Characterisation of Human Sapovirus from Children with Diarrhoea in the Rural Areas of Vhembe District, South Africa, 2017–2020

Magwalivha

Ngandu

Traore

et al. 2021

Viruses

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“…The frequency of HAdV-F infections detected in this study ( n =251; 57.8%) using NGS was higher than that observed in other studies carried out in Ethiopia ( n =450; 32 %) [23], Thailand ( n =2312; 7.2 %) [54], Kenya ( n =278; 37.4 %) [55], Iraq ( n =155; 34.2 %) [56], Iran ( n =2682; 5.2 %) [57], Bangladesh ( n =871; 10.7 %) [58], Albania ( n =142; 23.2 %) [59] and PR China ( n =2233; 10.9%) [60] using conventional PCR; Tanzania ( n =1235; 3.5 %) [27] and the Republic of Congo ( n =655; 5.5 %) [61] using an enzyme-linked immunosorbent assay (ELISA); and Turkey ( n =1154; 26.2 %) [62] using an immunochromatographic test. The HAdV-F frequency obtained here was also greater than that for previous studies conducted in Brazil using distinct screening methodologies: in São Paulo ( n =193; 3.1 %) (1987–1988) [63], Goiânia ( n =557; 2.1 %) (1987–1990) [64], Porto Velho (n-591; 2.0 %) (2010–2012) [65], Campo Grande ( n =415; 3.6 %) (2000–2004) [66], Belo Horizonte ( n =392; 4.5 %) (2005–2006) [67], Juiz de Fora ( n =377; .5 %) (2007–2011) [68], Curitiba ( n =255; 16.0 %) (2010–2011) [21], Belém do Pará ( n =172; 43.0 %) (1990–1992) [69], Porto Velho ( n =877; 6.4 %) (2000–2002) [70] and the midwestern, southeastern and southern regions ( n =3003; 3.9 %) in 2012–2017 [29]. This discrepancy concerning HAdV prevalence should be considered carefully and with caution.…”

Section: Discussionmentioning

confidence: 99%

Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics

Tahmasebi

Luchs

Tardy

et al. 2020

Journal of General Virology

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Human enteric adenovirus species F (HAdV-F) is one of the most common pathogens responsible for acute gastroenteritis worldwide. Brazil is a country with continental dimensions where continuous multiregional surveillance is vital to establish a more complete picture of the epidemiology of HAdV-F. The aim of the current study was to investigate the molecular epidemiology of HAdV-F using full-genome data in rural and low-income urban areas in northern Brazil. This will allow a genetic comparison between Brazilian and global HAdV-F strains. The frequency of HAdV-F infections in patients with gastroenteritis and molecular typing of positive samples within this period was also analysed. A total of 251 stool samples collected between 2010 and 2016 from patients with acute gastroenteritis were screened for HAdV-F using next-generation sequencing techniques. HAdV-F infection was detected in 57.8 % (145/251) of samples. A total of 137 positive samples belonged to HAdV-F41 and 7 to HAdV-F40. HAdV-F40/41 dual infection was found in one sample. Detection rates did not vary significantly according to the year. Single HAdV-F infections were detected in 21.9 % (55/251) of samples and mixed infections in 37.4 % (94/251), with RVA/HAdV-F being the most frequent association (21.5 %; 54/251). Genetic analysis indicated that the HAdV-F strains circulating in Brazil were closely related to worldwide strains, and the existence of some temporal order was not observed. This is the first large-scale HAdV-F study in Brazil in which whole-genome data and DNA sequence analyses were used to characterize HAdV-F strains. Expanding the viral genome database could improve overall genotyping success and assist the National Center for Biotechnology Information (NCBI)/GenBank in standardizing the HAdV genome records by providing a large set of annotated HAdV-F genomes.

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“…On the other hand, the lowest prevalence rate of HAdV infections in children (1.5%) was reported in the descriptive cross-sectional observational study conducted in Sudan by Wafa Elhag et al, 2013, which targeted children under 14 years old with acute diarrhea [ 51 ]. In comparison with other countries such as Australia, Brazil, Indonesia, Korea, Iran, the UK, Turkey, Hungary, and Sweden, the prevalence rates of HAdV infections ranged from 1% to 96.3% of patients [ 53 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

Human Mastadenovirus Infections in Children: A Review of the Current Status in the Arab World in the Middle East and North Africa

Qashqari

2022

Children

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Human mastadenovirus (HAdV) is a non-enveloped icosahedral virus with double-stranded DNA genomes. The mortality rate of HAdV infections can reach 35.5%, while gastroenteritis HAdV infections, HAdV pneumonia, and disseminated disease tend to show a worse outcome, with rates ranging from 44.2% to 50%. In addition, HAdV can cause infections at any age but most commonly in the pediatric population, especially in young children and infants. Therefore, this review aims to assess the current status of HAdV infections among children in the Arab World, particularly in the Middle East and North Africa. Web of Science, Scopus, PubMed, EMBASE, and Google Scholar databases for publications in English were searched up to July 2022 for relevant articles. The literature search yielded a total of 21 studies, which were included in this review. Studies reporting HAdV infections in children were conducted in 17 out of the 22 countries. The average prevalence rate of HAdV infections in children was 12.7%, with average prevalence rates of 12.82% and 12.58% in the Middle East and North African countries, respectively. The highest prevalence rate (28.3%) was reported in Egypt, whereas the lowest prevalence (1.5%) was reported in Sudan. The included studies presented children with signs and symptoms of gastroenteritis, acute respiratory infection, acute diarrhea, and acute hemorrhagic conjunctivitis. In conclusion, the average prevalence rate of HAdV infections in children was 12.7%, with average prevalence rates of 12.82% and 12.58% in the Middle East and North African countries, respectively. Finding the precise prevalence rate of this virus is crucial because it will guide future planning for effective disease control and the selection of particular treatment options during epidemics and special seasons.

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Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

Cited by 15 publications

References 38 publications

Prevalence and Genetic Characterisation of Human Sapovirus from Children with Diarrhoea in the Rural Areas of Vhembe District, South Africa, 2017–2020

Prevalence and Genetic Characterisation of Human Sapovirus from Children with Diarrhoea in the Rural Areas of Vhembe District, South Africa, 2017–2020

Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics

Human Mastadenovirus Infections in Children: A Review of the Current Status in the Arab World in the Middle East and North Africa

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