Adenosine Deaminase and Lymphocytic Populations

Baganha, M. Fontes; Pêgo, Alice; Lima, Maria A.; Gaspar, Elsa; Cordeiro, Antonio Robalo

doi:10.1378/chest.99.3.789-b

Cited by 26 publications

(40 citation statements)

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“…The diagnostic cut-off value for PF ADA in various studies has been reported in the range of 40-60 U/L. 16,18,19 Choosing a lower value increases sensitivity at the expense of specificity. From the ROC curve which indicates sensitivity and specificity at different cut-off levels, our study showed that at a cut-off value of 41 IU/L PF ADA the most widely used biomarker was found to exhibit sensitivity of 96% but at reduced specificity of 66%.…”

Section: Discussionmentioning

confidence: 99%

Markers for differentiation of tubercular pleural effusion from non-tubercular effusion

Ambade

Arora

Rai

et al. 2011

Medical Journal Armed Forces India

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BACKGROUNDThe limitations of the conventional methods for diagnosing tuberculosis (TB) have spurred multi-faceted research activities throughout the world.This study aims to explore the levels of adenosine deaminase (ADA) and interleukins in pleural effusion of tuberculous, malignant, and miscellaneous origin for differential diagnosis of tubercular and non-tubercular effusion. METHODAdenosine deaminase was estimated by kinetic method employing xanthine oxidase while interleukins were measured using commercially available ELISA kits in pleural fluids of tubercular and non-tubercular origin. RESULTSPleural fluids INF-γ, sIL-2R, TNF-α and ADA were significantly higher in TB group (n = 48) as compared to the non-TB group (n = 33) (mean ± SD:INF-γ; 1,958.7 ± 896.5 pg/mL vs 356.9 ± 733.6 pg/mL, sIL-2R; 6,101 ± 1,753.8 pg/mL vs 3,166 ± 2,611.1 ± pg/mL, TNF-α; 195.5 ± 292.1 pg/mL vs 59.7 ± 128.9 pg/mL, ADA; 123.6 ± 81.8 IU/L vs 48 ± 48.5 IU/L, P < 0.01). CONCLUSIONINF-γ is more sensitive and specific than ADA for the diagnosis of TB and should be added to the armamentarium of the diagnostic workup of pleural fluids for timely and accurate diagnosis of TB and differentiation of tubercular pleural effusion from non-tubercular effusion.

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Section: Discussionmentioning

confidence: 99%

Markers for differentiation of tubercular pleural effusion from non-tubercular effusion

Ambade

Arora

Rai

et al. 2011

Medical Journal Armed Forces India

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“…Therefore, an increase in ADA activity is present in several circumstances: (1) in pleural, pericardic, and peritoneal effusions of a tuberculous nature, significantly when compared with neoplastic and metapneumonic (bacterial and viral) ones; (2) in pleural effusions that follow rheumatoid arthritis, and lymphoproliferative infections and, also, in empyema, although with levels usually smaller than in tuberculosis; (3) in tuberculous cerebrospinal fluid, where ADA values are significantly higher than in normal group and in other neurologic diseases; and (4) in peripheral lymphoblasts of patients with acute lymphoid leukemia (Baganha et al, 1990). …”

Section: Adenosine Deaminase (Ada)mentioning

confidence: 99%

Adenosine Deaminase (ADA) in Tuberculous Meningitis

Skarar

Hossain

Shoab³

et al. 2015

Bangla J Med

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“…ADA aktivitesi tüm hücre tiplerinde bulunmakla birlikte, lenfoid dokular, timus ve periferal lenfositlerde daha fazladır. Bu enzim, lenfosit fonksiyonu ve T lenfositlerinin normal büyüme, farklı-laşma ve çoğalması için esansiyeldir [3] . GGT özellikle bellek T hücreleri olmak üzere T hücrelerinin aktivasyonunda artmaktadır [4] .…”

Section: Tartişma Ve Sonuçunclassified

“…ADA aktivitesi lenfositlerde eritrositlere oranla 10 kat daha fazla, T lenfositlerde ise B lenfositlere göre daha yüksek oranlarda bulunmaktadır. T hücrelerinin farklılaşması esnasında özellikle olgunlaşmamış ve farklılaş-mamış hücrelerde belirgin artış olmaktadır [3] . Leptospiroz olgularında ADA aktivitesinin belirlendiği çalışma sayısı çok azdır.…”

Section: Introductionunclassified

Leptospirozlu Sığırlarda Plazma Nitrik Oksit (NO) ve Tümör Nekrozis Faktör-α (TNF-α) Düzeyleri ile Adenozin Deaminaz (ADA), Gama Glutamil Transferaz (GGT) Aktiviteleri ve Perifer Kan Lökositlerinde Alfa Naftil Asetat Esteraz (ANAE) Yöntemiyle Lenfosit Oranlarının Belirlenmesi

Atakişi¹,

Kırmızıgül²,

Atakisi³

et al. 2014

Kafkas Univ Vet Fak Derg

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ÖzetYapılan bu çalışmada plazmada nitrik oksit (NO), Tümör Nekrozis Faktör-α (TNF-α) düzeyleri, adenozin deaminaz (ADA), gama glutamil transferaz (GGT) aktiviteleri ve alfa naftil asetat esteraz (ANAE) yöntemi ile lenfosit oranları belirlenerek, sığır leptospirozunun immunpatogenezinin ve biyokimyasal mekanizmalarının araştırılması amaçlanmıştır. Kars civarındaki köylerde yetiştirilen 20 baş leptospirozlu ve 20 baş sağlıklı danaların vena jugularislerinden heparinli tüplere kan örnekleri alındı ve histolojik analizler için frotiler alındıktan sonra, 3.000 rpm'de 15 dak. santrifüj edilerek plazmaları elde edildi. Plazmada NO düzeyi ile ADA aktivitesi kimyasal yöntemle, TNF-α düzeyi ile GGT aktivitesi ticari test kitleri kullanılarak kolorimetrik yöntemle analiz edildi. T/B lenfosit oranları ise perifer kan lökositlerinde ANAE yöntemiyle belirlendi. Leptospirozlu hayvanların plazma TNF-α ve NO düzeyleri kontrol grubuna göre sırasıyla P<0.001 ve P<0.05, ADA ve GGT aktiviteleri P<0.05 düzeyinde yüksek tespit edildi. ANAE pozitif (T) lenfosit oranı ise %43 olarak belirlendi. Sonuç olarak enfeksiyonun erken aşamasında saptanabilen bir sitokin olan TNF-α ve buna bağlı olarak NO düzeylerinin belirgin bir şekilde yükselmesi, ADA ve GGT aktivitelerinin artması ile B lenfosit yüzdesinin fazla olduğunun tespitinden yola çıkılarak, leptospirozun patogenezisinde bu parametrelerden yararlanılabileceği düşünülmektedir. Ayrıca doğal enfekte sığırlarda leptospirozun biyokimyasal mekanizmaları üzerine daha fazla çalışmaların yapılması gerektiği söylenebilir. SummaryIn this study, by determining in plasma the nitric oxide (NO), tumour necrosis factor-α (TNF-α) levels, adenosine deaminase (ADA), gamma glutamyl transferase (GGT) activities and percentage of T lymphocytes with alpha naphthyl acetate esterase method (ANAE), it was aimed to investigate the immunopathogenesis and biochemical mechanism of bovine leptospirosis. Blood samples of twenty healthy calves and twenty calves with leptospirosis which grown in the villages around Kars were collected into heparinised tubes from jugular vein. After taking the blood smear, blood samples were centrifuged at 3.000 rpm for 15 min. Plasma NO levels and ADA activities were determined with chemical method. TNF-α levels and GGT activities were determined with commercial kits. T/B lymphocyte ratios were determined at peripheral blood leukocytes with ANAE method. Plasma TNF-α and NO levels, ADA and GGT activities in animals with leptospirosis were found to be high P<0.001, P<0.05, P<0.05, P<0.05 respectively compared to healthy animals. ANAE positive T lymphocytes ratio were determined as 43%. As a result, TNF-α as a cytokine can be determined in early stage of infection and, accordingly, significantly increased levels of NO, ADA and GGT activities and, on the basis of the increased in the percentage of B lymphocytes, it is proposed to these parameters could be used for determining in explain the pathogenesis of the disease. It is tought that, it must be done more studies on biochemical mechan...

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Adenosine Deaminase and Lymphocytic Populations

Cited by 26 publications

References 0 publications

Markers for differentiation of tubercular pleural effusion from non-tubercular effusion

Markers for differentiation of tubercular pleural effusion from non-tubercular effusion

Adenosine Deaminase (ADA) in Tuberculous Meningitis

Leptospirozlu Sığırlarda Plazma Nitrik Oksit (NO) ve Tümör Nekrozis Faktör-α (TNF-α) Düzeyleri ile Adenozin Deaminaz (ADA), Gama Glutamil Transferaz (GGT) Aktiviteleri ve Perifer Kan Lökositlerinde Alfa Naftil Asetat Esteraz (ANAE) Yöntemiyle Lenfosit Oranlarının Belirlenmesi

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