Adenomatous Polyposis Coli Gene, ??-Catenin, and E-Cadherin Expression in Proximal and Distal Gastric Cancers and Precursor Lesions

Gulmann, Christian; Grace, Antoinette; Leader, M.; Butler, David M.; Patchett, Stephen; Kay, Elaine W.

doi:10.1097/00129039-200309000-00005

Cited by 10 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In order to determine whether β-catenin alterations could be detected early in gastric carcinogenesis, the present study focused on β-catenin expression in intestinal metaplasia not associated with gastric cancer. Unlike previous study [26] , in our series a reduction of β-catenin on the epithelial surface was observed in more than half of the patients. Intriguingly, a nuclear accumulation of β-catenin expression in 40% of patients with intestinal metaplasia was also found, and it was associated with reduced immunostaining at the intercellular boundaries in 75% of these cases.…”

Section: Discussion Discussion Discussion Discussion Discussioncontrasting

confidence: 94%

Relationship between b -catenin expression and epithelial cell proliferation in gastric mucosa with intestinal metaplasia

Romiti

Zullo²,

Borrini³

et al. 2005

WJG

View full text Add to dashboard Cite

Abstract Abstract Abstract AIM:To investigate β-catenin expression in patients with intestinal metaplasia, and to look for a possible relationship between β-catenin expression and either epithelial proliferation values or Helicobacter pylori (H pylori) infection. METHODS:Twenty patients with complete type intestinal metaplasia were studied. β-Catenin expression and epithelial cell proliferation in antral mucosa were assessed using an immunohistochemical analysis. H pylori infection was detected by histology and a rapid urease test. RESULTS:Reduced β-catenin expression on the surface of metaplastic cells was detected in 13 (65%) out of 20 patients. Moreover, in eight (40%) patients intranuclear expression of β-catenin was found. When patients were analyzed according to H pylori infection, the prevalence of both β-catenin reduction at the cell surface and its intranuclear localization did not significantly differ between infected and uninfected patients. Cell proliferation was higher in patients with intranuclear β-catenin expression as compared to the remaining patients, although the difference failed to reach the statistical significance (36±8.9 vs 27.2±11.4, P = 0.06). On the contrary, a similar cell proliferation value was observed between patients with reduced expression of β-catenin on cell surface and those with a normal expression (28.1±11.8 vs 26.1±8.8, P = 0.7). H pylori infection significantly increased cell proliferation (33.3±10.2% vs 24.6±7.4%, respectively, P = 0.04). CONCLUSION:Both cell surface reduction and intranuclear accumulation of β-catenin were detected in intestinal metaplasia. The intranuclear localization of β-catenin increases cell proliferation. H pylori infection does not seem to play a direct role in β-catenin alterations, whilst it significantly increases cell proliferation.

show abstract

Section: Discussion Discussion Discussion Discussion Discussioncontrasting

confidence: 94%

Relationship between b -catenin expression and epithelial cell proliferation in gastric mucosa with intestinal metaplasia

Romiti

Zullo²,

Borrini³

et al. 2005

WJG

View full text Add to dashboard Cite

show abstract

“…The immunostaining patterns of EMT-related proteins Snail and E-cadherin observed in GEJ adenocarcinoma are similar to the previously reported staining patterns of Snail and E-cadherin in gastric and esophageal cancers [8], [9], [11], [12], [30], [31]. The frequent lack of MTA3 expression in GEJ adenocarcinoma that we observed is consistent with its essential role in EMT as reported in breast, endometrial, and ovarian cancers [15], [18], [22], [24], [25].…”

Section: Discussionsupporting

confidence: 88%

The Metastasis-Associated Gene MTA3, a Component of the Mi-2/NuRD Transcriptional Repression Complex, Predicts Prognosis of Gastroesophageal Junction Adenocarcinoma

et al. 2013

View full text Add to dashboard Cite

Gastroesophageal junction (GEJ) adenocarcinoma carries a poor prognosis that is largely attributable to early and frequent metastasis. The acquisition of metastatic potential in cancer involves epithelial-to-mesenchymal transition (EMT). The metastasis-associated gene MTA3, a novel component of the Mi-2/NuRD transcriptional repression complex, was identified as master regulator of EMT through inhibition of Snail to increase E-cadherin expression in breast cancer. Here, we evaluated the expression pattern of the components of MTA3 pathway and the corresponding prognostic significance in GEJ adenocarcinoma. MTA3 expression was decreased at both protein and mRNA levels in tumor tissues compared to the non-tumorous and lowed MTA3 levels were noted in tumor cell lines with stronger metastatic potential. Immunohistochemical analysis of a cohort of 128 cases exhibited that patients with lower expression of MTA3 had poorer outcomes. Combined misexpression of MTA3, Snail and E-cadherin had stronger correlation with malignant properties. Collectively, results suggest that the MTA3-regulated EMT pathway is altered to favor EMT and, therefore, disease progression and that MTA3 expression was an independent prognostic factor in patients with GEJ adenocarcinoma.

show abstract

“…Já a correlação da densidade óptica com o índice de marcagem tanto de caspase-3 quanto de CD34 apresentaram resultados significantes com correlação linear positiva. Este fato sugere que os altos índices de marcagem para ambos os tumores expressa o nível de caspase-3 não ativada Há relatos 4,7 , que a incidência dos cânceres gástricos distais (corpo e antro) tem diminuído no mundo ocidental enquanto o proximal (cárdia e gastroesofágico) tem aumentado mais do que qualquer outro câncer. Esses tumores são morfologicamente indistinguíveis e a metaplasia intestinal parece ter importante papel na carcinogênese de ambos tumores.…”

Section: Expressão E Quantificação Dos Marcadores E O Local Do Tumor unclassified

CD34 e caspase-3: comparação, correlação de expressão e quantificação imunoistoquímica no adenocarcinoma gástrico

Silva¹,

Cuenca²,

Ribas-Filho³

et al. 2008

ABCD, arq. bras. cir. dig.

View full text Add to dashboard Cite

INTRODUÇAOO câncer gástrico continua a desafiar a medicina moderna em compreender seu comportamento biológico e a melhorar os índices de cura e de sobrevivência. O adenocarcinoma gástrico é o tipo histológico mais frequente (95%) e apresenta várias anomalias cromossômicas e gené-ticas. A análise citológica revela que tumores gástricos com grande proporção de células aneuplóides e com quantidade acima do normal de DNA nuclear, estão associados com mau prognóstico nos estágios precoce e avançados. Com o desenvolvimento da imunoistoquímica foi possível investigar novos métodos de estudo das alterações nucleares e citoplasmáticas.Novas técnicas de imunoistoquímica têm criado soluções como a categorização dos tumores indiferenciados, determinação do sítio de origem de metástases tumoral, e subclassificação de tumores em vários órgãos com maior exatidão histopatológica. A análise pelo sistema informatizado SAMBA 4000 vem contribuir para diminuir o grau de subjetividade dos métodos empregados .Este estudo tem por objetivos: 1. determinar e comparar a expressão e a quantificação dos imunomarcadores CD34 e caspase-3 no adenocarcinoma gástrico; 2. correlacionar a expressão e a quantificação deles com fatores clínicos e patológicos; 3. comparar e quantificar suas expressões nos adenocarcinomas gástricos proximais e distais. (4): 180-4 RESUMO -Racional -O câncer gástrico continua a desafiar a medicina moderna a compreender o seu comportamento biológico e a melhorar seus índices de cura cirúrgica e taxa de sobrevivência. Objetivo -Comparar e correlacionar a expressão e a quantificação dos marcadores caspase-3 e CD34 no adenocarcinoma gástrico com fatores clínico-patológicos e com os tumores gástricos proximais e distais. Método -Foi utilizada análise imunoistoquímica quantitativa com os anticorpos anti-caspase-3 policlonal de coelho e CD43 monoclonal de rato, para marcar células do adenocarcinoma gástrico em 26 casos com CD34 e 22 com caspase-3 oriundos de blocos parafinados. As lâminas foram lidas para os parâmetros índice de marcagem em porcentagem, e densidade óptica em pixels. A leitura foi realizada pelo sistema SAMBA de citofotometria de imagem. Resultados -As análises mostraram que a expressão dos marcadores caspase-3 e CD34 são elevadas, onde a média e o desvio-padrão para o índice de marcagem foram, respectivamente 87,72% ± 7,89 e 84,86% ± 9,17 e, para a densidade óptica, foram 60,75 ± 7,46 e 49,84 ± 8.48. Quando compararam-se os marcadores com a densidade óptica encontrou-se diferenças significativas entre a densidade óptica de caspase-3 (60,94) e a do CD34 (50,27) com P<0.001. A correlação entre os marcadores foi positiva para a densidade óptica de CD34 versus o índice de marcagem de CD34 com P=0.006, r=0,520 e para densidade óptica de caspase-3 versus índice de marcagem de caspase-3, com P=0.015, r=0.512. Não encontrou-se correlação dos marcadores quando compararam-se os índices de marcagem e as densidades ópticas com a curvatura gástrica, diferenciação tumoral, classificação de Borrmann, padrão tumoral ou invas...

show abstract

Adenomatous Polyposis Coli Gene, ??-Catenin, and E-Cadherin Expression in Proximal and Distal Gastric Cancers and Precursor Lesions

Cited by 10 publications

References 24 publications

Relationship between b -catenin expression and epithelial cell proliferation in gastric mucosa with intestinal metaplasia

Relationship between b -catenin expression and epithelial cell proliferation in gastric mucosa with intestinal metaplasia

The Metastasis-Associated Gene MTA3, a Component of the Mi-2/NuRD Transcriptional Repression Complex, Predicts Prognosis of Gastroesophageal Junction Adenocarcinoma

CD34 e caspase-3: comparação, correlação de expressão e quantificação imunoistoquímica no adenocarcinoma gástrico

Contact Info

Product

Resources

About