Additive Action of Royal Jelly and Honey Against<i>Staphylococcus aureus</i>

Boukraâ, Laïd; Niar, Abdellatif; Benbarek, H.; Benhanifia, Mokhtar

doi:10.1089/jmf.2007.567

Cited by 32 publications

(35 citation statements)

References 10 publications

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“…Many antimicrobial peptides have been discovered in the composition of RJ including royalactin, apisimin, jeleines I, II, III, IV, 10-HDA, and apalbumin α (2). In vitro studies have demonstrated that royalactin that is obtained from RJ was effective against gram positive bacteria including MRSA and some gram negative bacteria (3,5,14).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

Günaldı¹,

Dağlıoğlu²,

Tuğcu³

et al. 2013

Turkish Neurosurgery

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AIm: Implant-related infections are still a significant problem in spinal surgical procedures. Many drugs and methods have been tried to prevent implant-related infections. Our objective in this study was to evaluate whether royal jelly, which was found to hinder the growth of MRSA, has any preventive role in the prognosis of an infection in rats in an implant-related infection model. mATeRIAL and meTHods: Rats were divided into 3 groups of eight rats. Group-1 consisted of rats that underwent only a spinal implant, group-2 included those rats that were inoculated bacteria together with a spinal implant and group-3 was administered royal jelly in addition to a spinal implant and infection. ResuLTs:The amount of bacteria that grew in vertebral columns and implants was more in Group-2 than in Group-3, which meant that the number of bacteria colonies that grew was more quantitatively. This difference was found to be statistically significant in vertebral columns, but not in implants.CoNCLusIoN: Royal jelly could not fully prevent the MRSA infection in this model, but decreased the severity of infection noticeably. More objective and promising results may be obtained if royal jelly can be used at regular intervals in a different model to be designed with respect to implant-related infections.KeywoRds: Implant, Infection, Royal jelly, Spine, Staphylococcus aureus, Rat ÖZ AmAÇ: Spinal cerrahi prosedürlerde implant ilişkili enfeksiyonlar, hala önemli yer tutmaktadır. İmplant ilişkili enfeksiyonları önlemeye yönelik birçok ilaç ve yöntem uygulanmıştır. Arı sütünün, in-vitro ve in-vivo şartlarda metisilin rezistan stafilokokus aureusun üremesini engellediği tespit edilmiştir. Bu çalışmada, implant ilişkili spinal enfeksiyon modelinde arı sütünün etkisini değerlendirdik. yÖNTem ve GeReÇLeR: Sıçanlar sekizerli 3 gruba ayrıldı. Grup-1; sadece spinal implant uygulanan grup, Grup-2; spinal implantla beraber bakteri ekilen grup, Grup-3; spinal implant ve enfeksiyona ilave arı sütü uygulanan grup. Grup-1'deki sıçanların hiç birinde doku, implant ve kan kültürlerinde bakteriyel üreme olmadı. Grup-2 ve 3'teki sıçanların tamamında ekimi yapılan standart MRSA suşu üredi. Gruplar birbirleri arasında enfeksiyon yoğunluğu açısından karşılaştırıldı.BuLGuLAR: Grup-2'deki vertebral kolon ve implantlar üzerinde üreyen bakteri sayısının grup-3'e kıyasla daha şiddetli olduğu, üreyen bakteri koloni sayısının kantitatif olarak daha fazla olduğu tespit edildi. Bu fark, vertebral kolonda istatistiksel olarak anlamlı bulunurken, implant üzerinde anlamlı bulunmadı. soNuÇ: Arı sütü bu modelde, MRSA enfeksiyonunu tam olarak önleyememiş ancak, enfeksiyonun şiddetini belirgin olarak azaltmıştır. Arı sütün implant ilişkili enfeksiyonlarda farklı bir model oluşturularak, düzenli aralıklarla kullanılabilmesi durumunda daha objektif ve umut verici sonuçlar ortaya konabilir. Ayrıca arı sütün, farklı modeller oluşturularak, farklı bakterilerde ve diğer Staph. aureus suşlarında da denenmesinin uygun olacağı kanaatindeyiz.

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Section: Discussionmentioning

confidence: 99%

Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

Günaldı¹,

Dağlıoğlu²,

Tuğcu³

et al. 2013

Turkish Neurosurgery

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“…For both types of honeys-Italian and Spanish Forest honeys, it was found to rapidly clear the infection in the wound, and promote and clean healthy granulation tissue. The antimicrobial activity of the combination of RJ and honey was studied [37,38], and the additive activity of RJ and honey against P. aeruginosa was found, as comparison with the experiments in this study, where the water soluble F-RJ (M) did not affect the Forest honey in term of statistically stronger antimicrobial activity. Al-Waili et al [39] also analysed the antimicrobial activity of the combination of ethanol extract of propolis and honey from Saudi Arabia, and found the synergism between the ethanol extract of Saudi propolis or Egyptian propolis with honey from Saudi Arabia in term of killing of MRSA, E. coli and C. albicans.…”

Section: The Growth Promoting Activity Of Tlt Cell Line By the Combinmentioning

confidence: 52%

Water Soluble Propolis and Royal Jelly Enhance the Antimicrobial Activity of Honeys and Promote the Growth of Human Macrophage Cell Line

Filipič¹,

Gradišnik²,

Ružić‐Sabljić³

et al. 2016

JAST-B

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Due to the overuse and misuse of antibiotic, an increase in antibiotic resistance of pathogenic bacteria is evolving. Attention should be focused on natural alternatives to antibiotics, like propolis, royal jelly (RJ) and honeys. They all have strong antibacterial properties due to the active substances they contain. This study investigated the effect of combination of water soluble propolis (WSP) Greit120 or fresh royal jelly (F-RJ) (Mižigoj) and Forest honeys as antibacterial against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinetobacter baumanii, Staphylococcus aureus, methicillin resistant Staphylococcus aureus (MRSA), Streptococcus pyogenes, Streptococcus agalactiae and Candida albicans. These substances are also cell growth promoters for human macrophage (TLT) cell line. WSP Greit120, F-RJ (M) and different Forest honeys were prepared in saline as 10% solutions. The antimicrobial activity was expressed as the minimal inhibitory concentration (MIC) in mg/mL. The growth promotion activity was measured at optical density (OD) 595 nm. The combination of WSP Greit120 with different Forest honeys is better than F-RJ (M) in same combination with different Forest honeys. The best antibacterial/antifungal activity was found with the combination of 10% WSP Greit120 in the Forest honey (1:10) from Italy or Spain. When measuring the growth promoting activity of TLT cell line, the best activity was detected at the combination of 10% WSP Greit120 in the Forest honey from Italy (GI 3 = 0.796 ± 0.014 and GI 5 = 1.133 ± 0.022). Antimicrobial and growth promoting activities are correlated and WSP-dependent.

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“…3.34 CFU/mL >8 log CFU/mL >8 log CFU/mL 20% >8 log CFU/mL >8 log CFU/mL 30% 3.53 CFU/mL 1.39 CFU/mL 40% 0 0 RJ 10% 3.34 CFU/mL >8 log CFU/mL >8 log CFU/mL 20% 0 0 30% 0 0 RJ:RH (1:100) 10% 3.34 CFU/mL >8 log CFU/mL >8 log CFU/mL 20% 3.58 log CFU/mL 2.11 log CFU/mL 30% 0 0 40% 0 0 3.17 CFU/mL >7 log CFU/mL >7 log CFU/mL 20% >7 log CFU/mL >7 log CFU/mL 30% >7 log CFU/mL >7 log CFU/mL 3.17 CFU/mL >7 log CFU/mL >7 log CFU/mL 20% >7 log CFU/mL >7 log CFU/mL 30% >7 log CFU/mL >7 log CFU/mL 40% 0 3.47 log CFU/mL (v/v). When honey and RJ were used jointly, all honey varieties had a more than 50% decrease in MIC with 1% (v/v) RJ (Boukraa et al, 2008). In another study in Algeria, the MIC of RJ was 1.7% (vol /vol) against S. aureus and 2% against Escherichia coli (Boukraa et al, 2009).…”

Section: Resultsmentioning

confidence: 96%

Effect of Solvent on Aflatoxin Content in Extracts of Virginia Type Peanut Skins

Sarnoski

2015

J Food Health Sci

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Abstract:Antimicrobial resistance of pathogenic bacteria is a growing public health problem. Methicillin-resistant Staphylococcus aureus (MRSA) represents a significant burden on healthcare services because it is involved in severe difficult to treat infections in humans. Several non-antibiotic approaches regarding the treatment of infections caused by MRSA as well as of other resistant bacteria have been studied. Since ancient times royal jelly and honey have been considered both as foods and medicines, and recently have been identified as potential alternative medicines to treat bacterial infections, especially of the skin and soft tissue. The aim of the present study was to investigate the antibacterial effect of honey, royal jelly and their mixtures against MRSA in vitro. At least 3 decimal reductions of MRSA count were observed in Tryptone Soy broth with concentrations of 40% rape honey (RP), 20% and 30% royal jelly (RJ), 30% RJ:RH (1:100). In general, honey and royal jelly, individually or in combination, appeared to have a potential as alternative therapeutic agents against MRSA infections, but clinical studies are needed for confirmation.

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Additive Action of Royal Jelly and Honey AgainstStaphylococcus aureus

Cited by 32 publications

References 10 publications

Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

Antibacterial effect of royal jelly for preservation of implant-releated spinal infection in rat

Water Soluble Propolis and Royal Jelly Enhance the Antimicrobial Activity of Honeys and Promote the Growth of Human Macrophage Cell Line

Effect of Solvent on Aflatoxin Content in Extracts of Virginia Type Peanut Skins

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