Additional random, single to multiple genome fragments of Penaeus stylirostris densovirus in the giant tiger shrimp genome have implications for viral disease diagnosis

Saksmerprome, Vanvimon; Jitrakorn, Sarocha; Chayaburakul, Kanokporn; Laiphrom, Seansook; Boonsua, Khanittha; Flegel, Timothy W.

doi:10.1016/j.virusres.2011.06.010

Cited by 49 publications

(43 citation statements)

References 15 publications

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“…Subsequently, many more EVE for IHHNV were reported for P. monodon and P. vannamei and many of them gave false‐positive PCR test results for IHHNV using the OIE recommended detection method, even though the shrimp were not infected with IHHNV (Saksmerprome et al . ; Brock et al . ).…”

Section: Problems Confirming Spf Statusmentioning

confidence: 96%

Facts, truths and myths about SPF shrimp in Aquaculture

Alday-Sanz¹,

Brock

Flegel

et al. 2018

Reviews in Aquaculture

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Shrimp domestication and genetic improvement programmes began in late 1980s, in the United States of America, under the United States Marine Shrimp Farming Program (USMSFP), using the Pacific whiteleg shrimp Penaeus vannamei. The USMSFP was based on proven concepts from the livestock and poultry industries and began with establishing a specific pathogen‐free (SPF) shrimp stock. The original shrimp stock was obtained using rigorous screening of captured wild shrimp for selection of individuals naturally free of major shrimp pathogens. Although the concept of SPF animals was well defined for terrestrial animals, it was relatively new for aquaculture, and it took some time to be adopted by the aquaculture community. In the early 1990s, parallel to USMSFP, several other programmes on genetic improvement of shrimp were also initiated in Latin America. Subsequently, several new terminologies and products, such as specific pathogen resistant (SPR) shrimp, specific pathogen tolerant (SPT) shrimp and even ‘all pathogen exposed’ (APE) shrimp, entered the shrimp industry vocabulary and became commercial. This led to confusion in the shrimp industry about the meaning, relationship and significance of these new terms with respect to SPF. This position paper attempts to clarify these concepts, provide science‐based definitions, reconfirms the importance of developing, maintaining and using domesticated, specific pathogen‐free (SPF) shrimp stocks (which may also achieve SPR and/or SPT status) to reduce the risk of disease outbreaks and increase production and profit. The same principles would apply to development of domesticated SPF stocks for other species used in aquaculture. The paper also discusses the difficulties of confirming and certifying SPF status due to the presence of endogenous viral elements (EVEs) and calls for internationally agreed science and evidence‐based technical guidelines for producing healthy shrimp.

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Section: Problems Confirming Spf Statusmentioning

confidence: 96%

Facts, truths and myths about SPF shrimp in Aquaculture

Alday-Sanz¹,

Brock

Flegel

et al. 2018

Reviews in Aquaculture

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show abstract

“…Because some IHHNV‐related sequences are integrated into the shrimp genome (Saksmerprome et al., ; Tang & Lightner, ), current PCR methods for detecting IHHN may yield false‐positive results. Therefore, screening for IHHN requires a larger target DNA region to exclude the integrated sequences.…”

Section: Primer Sets Used For Pcr‐dna Chromatographymentioning

confidence: 99%

A rapid method for simultaneously diagnosing four shrimp diseases using PCR‐DNA chromatography method

Koiwai

Kodera

Thawonsuwan

et al. 2017

Journal of Fish Diseases

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“…One was the one-step method employing 309F/R primers (Tang et al, 2007) which was certified by OIE in the Manual of Diagnostic Tests for Aquatic Animals 2010 (http://www.oie.int/ international-standard-setting/aquatic-manual/access-online/) which states, "Primer set 309F/R amplifies only a segment from IHHNV types 1 and 2 (the infectious forms of IHHNV), but not types 3A and 3B, which are non-infectious and part of the P. monodon genome." The other was the interim 3-tube nested PCR by Saksmerprome et al (2011) who claim that, "This method employed 3 overlapping primer sets designed to cover 89.5% of the IHHNV genome such that positive result for the all three sets from a shrimp specimen would indicate the presence of infectious IHHNV". To determine the detection sensitivity, the same set of total DNA dilutions tested by RPA-LFD was amplified by the Table 2 Diagnostic performance of the RPA-LFD protocol.…”

Section: Molecular Sensitivity Of One-step Pcr and Nested Pcr By Agementioning

confidence: 99%

Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome

Jaroenram

Owens

2014

Journal of Virological Methods

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Additional random, single to multiple genome fragments of Penaeus stylirostris densovirus in the giant tiger shrimp genome have implications for viral disease diagnosis

Cited by 49 publications

References 15 publications

Facts, truths and myths about SPF shrimp in Aquaculture

Facts, truths and myths about SPF shrimp in Aquaculture

A rapid method for simultaneously diagnosing four shrimp diseases using PCR‐DNA chromatography method

Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome

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