A rapid method for simultaneously diagnosing four shrimp diseases using <scp>PCR</scp>‐<scp>DNA</scp> chromatography method

Koiwai, Keiichiro; Kodera, Takuya; Thawonsuwan, Jumroensri; Kawase, Makoto; Kondo, Hidehiro; Hirono, Ikuo

doi:10.1111/jfd.12732

Cited by 14 publications

(8 citation statements)

References 15 publications

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“…The developed STH-PAS method in its current state is specific for a single point mutation in the katG gene at codon 315, and does not include other mutations conferring resistance to INH. Nonetheless, studies are still on-going to include the other resistant mutations for INH as the strip can detect multiple PCR products at once (13). The inhA promotor mutation will be included in our future development.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Rapid and Simple Detection of Isoniazid-Resistant <i>Mycobacterium tuberculosis</i> Utilizing a DNA Chromatography-Based Technique

Kodera¹,

Yamaguchi

Fukushima

et al. 2021

Jpn J Infect Dis

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Section: Accepted Manuscriptmentioning

confidence: 99%

Rapid and Simple Detection of Isoniazid-Resistant <i>Mycobacterium tuberculosis</i> Utilizing a DNA Chromatography-Based Technique

Kodera¹,

Yamaguchi

Fukushima

et al. 2021

Jpn J Infect Dis

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“…In addition, previous studies have shown that the PCR–dipstick chromatography method is more sensitive than gel electrophoresis [ 19 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Design and Evaluation of Multiplex One-Step Reverse Transcription PCR–Dipstick Chromatography Method for the Analysis of Seven Respiratory Pathogens

et al. 2021

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Influenza A, influenza B, severe acute respiratory syndrome coronavirus 2, adenovirus, respiratory syncytial virus, Mycoplasma pneumoniae , and Chlamydophila pneumoniae are common pathogens that can cause severe pneumonia and other symptoms, resulting in acute lower respiratory tract infections. The objective of this study was to design and evaluate a sensitive and specific multiplex one-step reverse transcription PCR (RT-PCR)–dipstick chromatography method for simultaneous rapid detection of these seven pathogens. Streptavidin-coated blue latex particles were used to read out a positive signal. Based on the DNA–DNA hybridization of oligonucleotide sequences (Tag) for forward primer with the complementary oligonucleotide sequence (cTag) on the dipstick and biotin–streptavidin interactions, PCR products were able to be illuminated visually on the dipstick. The specificity and the limit of detection (LOD) were also evaluated. Moreover, the clinical performance of this method was compared with Sanger sequencing for 896 samples. No cross reaction with other pathogens was found, confirming the high specificity of this method. The LOD was 10 copies/µL for each of the tested pathogens, and the whole procedure took less than 40 min. Using 896 samples, the sensitivity and specificity were shown to be no lower than 94.5%. The positive predictive value was higher than 82.1%, and the negative predictive value was higher than 99.5%. The kappa value between the PCR–dipstick chromatography method and Sanger sequencing ranged from 0.869 to 0.940. In summary, our one-step RT-PCR–dipstick chromatography method is a sensitive and specific tool for rapidly detecting multiplex respiratory pathogens. Supplementary Information The online version contains supplementary material available at 10.1007/s00284-021-02621-7.

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“…Additionally, recent studies were able to develop an on-site detection method for V. parahaemolyticus AHPND (Arunrut et al 2016;Koiwai et al 2016;Kongrueng et al 2015). Moreover, a method using PCR-DNA chromatography along with multiplex PCR was used to identify AHPND, hypodermal and hepatopancreatic necrosis infections, enterocytozoon hepatopenaei, and white spot disease concurrently (Koiwai et al 2018).…”

Section: Polymerase Chain Reaction and Loop-meditated Isothermal Amplmentioning

confidence: 99%

Diagnosis and potential treatments for acute hepatopancreatic necrosis disease (AHPND): a review

et al. 2019

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Acute hepatopancreatic necrosis disease (AHPND) or formerly known as early mortality syndrome (EMS) is an emerging disease that has caused significant economic losses to the aquaculture industry. The primary causative agent of AHPND is Vibrio parahaemolyticus, a Gram-negative rod-shaped bacterium that has gained plasmids encoding the fatal binary toxins Pir A/Pir B that cause rapid death of the infected shrimp. In this review, the current research studies and information about AHPND in shrimps have been presented. Molecular diagnostic tools and potential treatments regarding AHPND were also included. This review also includes relevant findings which may serve as guidelines that can help for further investigation and studies on AHPND or other shrimp diseases.

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A rapid method for simultaneously diagnosing four shrimp diseases using PCR‐DNA chromatography method

Cited by 14 publications

References 15 publications

Rapid and Simple Detection of Isoniazid-Resistant <i>Mycobacterium tuberculosis</i> Utilizing a DNA Chromatography-Based Technique

Rapid and Simple Detection of Isoniazid-Resistant <i>Mycobacterium tuberculosis</i> Utilizing a DNA Chromatography-Based Technique

Design and Evaluation of Multiplex One-Step Reverse Transcription PCR–Dipstick Chromatography Method for the Analysis of Seven Respiratory Pathogens

Diagnosis and potential treatments for acute hepatopancreatic necrosis disease (AHPND): a review

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