Addition of L-arginine to the fertilization medium enhances subsequent bovine embryo development rates

Santana, P. P. B.; Silva, Bruno B. da; Silva, Thiago V.G.; Costa, N. N.; Cordeiro, M. S.; Santos, S. S. D.; Ohashi, Otávio Mitio; Miranda, M. S.

doi:10.1016/j.theriogenology.2015.11.027

Cited by 9 publications

(13 citation statements)

References 49 publications

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“…On in vitro embryo production, no difference was observed in the cleavage rate between treatments with heparin in the absence of COC comparing to Control IVF, corroborating with the results obtained by Santana et al (2016) and Leal et al (2012). The group treated with L-arg demonstrated the lowest cleavage rate, as well as the lowest rate of capacitation and in vitro production of embryos.…”

Section: Discussionsupporting

confidence: 88%

“…In the same way, the primary source of NO production for the sperm capacitation in vivo originates from the female reproductive tract (Lefièvre et al, 2007), being concordant with the improvement observed by the addition of L-arg to the capacitating medium. Although the NO dosage has not been performed in our research, many studies demonstrate the NO synthesis by cattle sperm using L-arg as a substrate during the in vitro capacitation (Leal et al, 2009;Santana et al, 2016).…”

Section: Discussionmentioning

confidence: 98%

“…Although L-arg has been widely used in sperm capacitation (Funahashi, 2002;O'Flaherty et al, 2004;Leal et al, 2009), most of the studies do not evaluate its effect after capacitation, such as the in vitro embryo production. In a recent publication, L-arg improved embryonic developmental rates in cattle when added to the medium of IVF (Santana et al, 2016). Although common IVF medium is enriched to stimulate sperm capacitation, in this model, L-arg could influence both sperm and COCs.…”

Section: Introductionmentioning

confidence: 87%

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Association of L-arginine with heparin on the sperm capacitation improves in vitro embryo production in bovine

Aguiar¹,

Caldas-Bussiere²,

Maciel³

et al. 2019

Anim Reprod

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We aimed to evaluate the effects of L-arginine (L-arg) in the quality of in vitro heparin-induced capacitation of cryopreserved bovine spermatozoa and its effects on IVP. The experimental groups were: Control 0 hour without pre-capacitation, and groups of sperm capacitated for 30 min in the absence of COC with heparin (Control 30 min), with 1 mM L-arg and with 1 mM L-arg + heparin. The capacitation pattern was evaluated by chlortetracycline assay and the integrity of the plasma membrane (PM) and acrosome membrane (AM) by the association of Hoescht 33342 and propidium iodide. Further, we assess the sperm quality by the rate of in vitro blastocysts production. Treatment with 1 mM L-arg + heparin increased the percentage of capacitated sperm when compared to Control 0 hour and the treatment with heparin (61.1 vs. 18.2 and 47.0%, respectively, P<0.05). The addition of 1 mM L-arg to the medium has capacitated the spermatozoa (26.2 ± 3.8) but was less effective than heparin (47.0 ± 4.0) (P<0.05). There was no difference in the percentage of sperm with intact PM between treatments when compared to Control 0 hour (P>0.05). The group capacitated with 1 mM L-arg + heparin for 30 min increased the blastocyst rate compared to Control IVF (53.7 vs. 40.8%, P<0.05). We conclude that the addition of L-arg with heparin increases the number of capacitated spermatozoa in vitro with 30 min of pre-incubation in the absence of COC not altering the integrity of plasma and acrosomal membrane. This treatment in the absence of COC was the most effective method for blastocysts production, and the method of pre-incubation could be used to assess the role of other substances in the sperm capacitation and its effect on IVP.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 87%

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Association of L-arginine with heparin on the sperm capacitation improves in vitro embryo production in bovine

Aguiar¹,

Caldas-Bussiere²,

Maciel³

et al. 2019

Anim Reprod

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“…The importance of NO for correct embryonic development was observed, mainly for transition from morulae to the blastocyst stage. Treatment of embryos with the NOS inhibitor l-NAME reduced blastocysts numbers and hatching rates [42]. Contrarily, exposing bovine mature oocytes to a nitric oxide donor short term did not induce stress tolerance and had no positive effect on the in vitro embryo production of bovine embryos, as was expected [43].…”

Section: No In Bovine Oocytes Eggs and Embryosmentioning

confidence: 72%

From Nitric Oxide Toward S-Nitrosylation: Expanding Roles in Gametes and Embryos

Ješeta¹,

Sedmı́ková²,

Bodart³

2017

Nitric Oxide Synthase - Simple Enzyme-Complex Roles

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Nitric oxide (NO) is a gasotransmitter involved in various aspects of reproduction. The observational data from different species, such as sea urchin, ascidians, amphibians, rodents, porcine, bovine, and human, suggest that NO might have a significant role in reproduction through several mechanisms. This proposed role might appear preserved through evolution; however, the effects of NO also depend on the species or stages considered. There has been debate over the physiological relevance of NO, though the benefits of its use in assisted reproduction are now widely recognized. Over the past years, S-nitrosylation has provided a new angle to decipher the mechanisms through which NO exerts its actions. This chapter summarizes, in a nonexhaustive manner, research that explores the role of NO in gametes and embryos.

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“…A semen sample was submitted to cold thermal shock and was used as a positive control, and an aliquot of sperm that were not stained with fluorescent probes was used as a negative control (SANTANA et al, 2016).…”

Section: Evaluation Of Plasmatic and Acrosomal Membrane Integrity Andmentioning

confidence: 99%

Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm

Reis

Miranda

Silva

et al. 2017

SCA

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Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. Key words: Powdered Coconut Water. Lippia. Cryopreservation. Spermatozoa. Buffalo. ResumoPara a implantação da inseminação artificial é indispensável à utilização de sêmen congelado, que pode provocar mudanças deletérias na estrutura e na integridade das membranas espermáticas, comprometendo sua função. Para evitar estes danos celulares, há a necessidade de se utilizar meios diluidores e substratos adequados que recuperem o maior número possível de células viáveis pós-descongelação. Para isso, foram avaliados, no experimento I, três diferentes diluidores, o diluidor TES-TRIS, bastante utilizado para bubalinos, e um diluidor a base de água de coco em pó (ACP-112  ), associado ou não ao leite (ACP-112  -Leite), na congelação do sêmen de bubalinos; e no experimento II, foi avaliado o efeito do óleo extraído da Lippia origanoides na proteção dos espermatozóides contra as crioinjúrias decorrentes da congelação do sêmen bubalino. Foram utilizados 10 touros bubalinos para as colheitas de sêmen (10 ejaculados/touro), sendo os ejaculados diluídos em TES-TRIS (controle), ACP-112  e ACP-112  -Leite no experimento I; e no experimento II, os ejaculados foram diluídos no melhor diluidor obtido no experimento I, acrescido de 2.5 µg mL -1 , 5 µg mL -1 e 10 µg mL -1 da planta e o grupo controle, constituído somente do diluidor. O sêmen recém colhido foi analisado quanto as características convencionais, tais como, motilidade, concentração, morfologia e viabilidade. Após a descongelação das amostras foram avaliados novamente, motilidade e viabilidade espermática, e posteriormente, foram realizados os testes de termo-resistência, hiposmótico e de avaliação das membranas dos espermatozóides, através das sondas fluorescentes PI, FITC-PSA e JC-1, utilizando a citometria de fluxo. Os dados obtidos foram submetidos à ANOVA e ao Teste de Tukey a 5%. No experimento I, o diluente TES-TRIS apresentou melhores resultados para as várias características avaliadas quando comparado com o ACP-112  e ACP-112  -Leite (P < 0.05), demonstrando maior proteção deste diluidor às estruturas espermáticas durante a criopreservação do sêmen de bubalinos. No experimento II, as diferentes concentrações do óleo extraído da Lippia origanoides não demonstraram nenhuma diferença (P > 0.05) entre as variáveis avaliadas, quanto à proteção das estruturas espermáticas durante a criopreservação. Com base nestes dados, pôde-se demonstrar os efeitos benéficos do diluidor TES-TRIS na qualidade pós-descongelação dos espermatozóides bubalinos; entretanto, nenhum efeito protetor foi observado sobre os espermatozóides bubalinos criopreservados em associação com o óleo extraído da Lippia origanoides, nas diferentes concentrações utilizadas. Palavras-chave: Água de coco em pó. Lippia. Criopreservação. Espermatozóid...

show abstract

Addition of L-arginine to the fertilization medium enhances subsequent bovine embryo development rates

Cited by 9 publications

References 49 publications

Association of L-arginine with heparin on the sperm capacitation improves in vitro embryo production in bovine

Association of L-arginine with heparin on the sperm capacitation improves in vitro embryo production in bovine

From Nitric Oxide Toward S-Nitrosylation: Expanding Roles in Gametes and Embryos

Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm

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