Addition of Glutathione to an Extender for Frozen Equine Semen

Oliveira, Rodrigo Arruda de; Wolf, C.A.; Viu, Marco Antônio de Oliveira; Gambarini, M. L.

doi:10.1016/j.jevs.2013.05.001

Cited by 44 publications

(40 citation statements)

References 17 publications

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“…There is no consensus in the scientific literature regarding the effects of glutathione when added to a cooling or freezing extender, since some studies report positive effects in swine (GADEA et al, 2005) and equines (OLIVEIRA et al, 2013), and no significant effects in other species (BAUMBER et al, 2005). The disparity in the results of the present study and those of the cited experiments could be attributed to variations in age, breed, species, and composition of the extender in which glutathione was incorporated.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies on cooling and freezability of equine semen at 5ºC have shown the beneficial effects of glutathione including improved motility, viability, and membrane integrity (OLIVEIRA et al, 2013;ZHANDI;GHADIMI, 2014). Thus, the present study aimed to evaluate the in vitro effects of adding glutathione to equine sperm during refrigeration at 16ºC for 36 hours.…”

Section: Introductionmentioning

confidence: 99%

“…Oxidative effects caused by ROS can be reduced by adding antioxidants to seminal plasma or extenders used for freezing (BAUMBER et al, 2005;OLIVEIRA et al, 2013;ZHANDI;GHADIMI, 2014). Glutathione (ɣ-L-glutamyl-lcysteinylglycine), which is a tripeptide thiol, widely found throughout the animal body in both somatic cells and gametes, with numerous biological functions, is one of the antioxidants incorporated into the semen of some species.…”

Section: Introductionmentioning

confidence: 99%

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Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations

2015

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Handling equine semen during the refrigeration process reduces sperm viability, and consequently causes membrane lipid peroxidation, among other challenges. The present study aimed to evaluate the in vitro effects of glutathione (control, 1. 0, 1. 5, and 2. 5 mM) on equine semen in a cooled protocol of 16ºC for 36 hours. The following variables were evaluated after 0, 12, 24, and 36 hours refrigeration: total sperm motility, vigor, viability, and plasma and acrosomal membrane integrity. Motility was higher with 2. 5mM of glutathione (57. 8 ± 7. 3) after 12 hours of refrigeration compared to the control (53. 2 ± 8. 3) (P < 0. 05). After 36 hours of refrigeration, motility was higher with 1. 5 mM (43. 4 ± 12. 7) and 2. 5mM glutathione (45. 5 ± 6. 2), than it was with 1mM glutathione (38. 2 ± 9) and the control (35. 5 ± 18. 4) (P < 0. 05), respectively. The strength was highest with 1. 5mM glutathione (3. 7 ± 0. 3) after 36 hours compared to the control (3. 2 ± 1. 1), (P < 0. 05). Viability differed between control and 1mM treatments (79. 5 ± 1. 8) only after 24 hours (75. 5 ± 9. 7) (P < 0. 05). Throughout the investigation, no significant differences were noted in plasma and acrosomal membrane integrity (P > 0. 05). The 1. 5 and 2. 5mM glutathione levels were more efficient in protecting sperm cells and yielded higher total motility values after 36 hours of refrigeration. Key words: Antioxidant, sperm, stallion, cooled semen ResumoA manipulação do sêmen equino durante o processo de refrigeração reduz a viabilidade espermática em consequência, entre outros problemas, da peroxidação de lipídios da membrana. Objetivou-se avaliar o efeito in vitro da adição de glutationa (controle; 1,0; 1,5 e 2,5mM) em protocolo de refrigeração a 16ºC por 36h. As variáveis avaliadas foram motilidade total, vigor, viabilidade espermática e integridade das membranas plasmática e acrossomal em quatro momentos diferentes de refrigeração (0, 12, 24 e 36h). A motilidade foi superior com 2,5mM de glutationa (57,8±7,3) com 12h de refrigeração quando comparado ao controle (53,2±8,3), P<0,05. Com 36h de refrigeração a motilidade foi superior com 1,5mM (43,4±12,7) e 2,5mM (45,5±6,2) quando comparado com 1mM (38,2±9) e ao controle (35,5±18,4), P<0,05. Com relação ao vigor houve superioridade com 1,5mM de glutationa (3,7±0,3), na avaliação de 36h, em comparação ao controle (3,2±1,1), P<0,05. Para viabilidade só houve diferença em relação ao controle na avaliação de 24h (75,5±9,7) quando comparado ao tratamento 1mM (79,5±1,8), P<0,05. Não houve diferença em relação ao controle para as avaliações de integridade de membrana plasmática e acrosomal, em todos os momentos de avaliação, P>0,05. As concentrações de 1,5 e 2,5MM de glutationa foram mais eficientes para proteção da célula espermática com valores superiores de motilidade total na avaliação de 36h. Palavras-chave: Antioxidante, espermatozoide, garanhão, sêmen refrigerado

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations

2015

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“…Converting glutathione -reduced form (GSH) to Glutathione -oxidized form (GSSG), which contains two molecules linked by a disulfide bond. GPx contains a selenium atom, bonded covalently in the form of selenocysteine, that is essential for this enzyme carries out its activity (Nordberg & Árner, 2001;Maiorino et al, 2003;Oliveira et al, 2013).…”

Section: Glutathione Peroxidase Selenium and Others Trace Elementsmentioning

confidence: 99%

Nutraceutical in male reproduction

Freitas¹,

Oliveira

2018

Braz. J. Vet. Med.

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In order to obtain a successful outcome in artificial insemination programs, a few precautions must be taken with regard to male breeders. It is established that environmental factors can change the hormonal secretion, the cell differentiation that occurs in the testicles, as well as sperm maturation and transport in the epididymis. In view of adverse nutritional factors, reproductive organs can present degeneration and disorders in different degrees and intensities, affecting the animal fertility. The term nutraceutical describes products derived from foods, which may provide additional health benefits, beyond the basic value found in diets. Among the main nutraceuticals used in male reproduction, there are omega-3, arginine, B-complex vitamins, L-carnitine, β-carotene and antioxidants. The aim of this paper is to present the main substances used as nutraceuticals, in order to improve semen quality, obtaining most favorable outcomes of stallions in the use of natural breeding or artificial insemination.Keywords: artificial insemination, fertility, nutrition, semen, spermatogenesis.resumo Para um sucesso em programas de inseminação artificial, algumas precauções devem ser tomadas em relação aos reprodutores machos. Sabe-se que fatores ambientais podem alterar a secreção hormonal, a diferenciação celular que ocorre nos testículos, assim como o transporte e a maturação espermática no epidídimo. Um déficit nutricional pode levar a degeneração e distúrbios dos órgãos reprodutivos em diferentes graus e intensidade, afetando a fertilidade. O termo nutracêutico descreve produtos derivados dos alimentos, que podem fornecer benefícios adicionais a saúde, além dos valores básicos encontrados na dieta. Entre os vários nutracêuticos utilizados na reprodução, temos o ômega-3, arginina, vitaminas do complexo B, L-carnitina, β-caroteno e antioxidantes. Objetivou-se com essa revisão apresentar as principais substâncias utilizadas como nutracêuticos, com a finalidade de melhorar a qualidade seminal, obtendo resultados favoráveis dos garanhões com a monta natural ou a inseminação artificial.Palavras-chave: inseminação artificial, fertilidade, nutrição, sêmen, espermatogênese.

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“…MACIAS-GARCIA, T. GUIMARÃES, G. LOPES, A. ROCHA, L. GONZALEZ-FERNANDEZ any benefit on equine sperm quality after thawing. In addition, other ROS scavengers such as glutathione have resulted in moderate beneficial effects in equine sperm (de Oliveira et al, 2013). Genistein is a phytoestrogen belonging to the isoflavone category with antioxidant properties and it is an effective hydrogen peroxide scavenger (Record et al, 1995) as well as a potent inhibitor of protein tyrosine kinases (Gonzalez-Fernandez et al, 2013).…”

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confidence: 99%

Effect of genistein addition to equine sperm freezing extender

Macías‐García

Guimarães

Lopes

et al. 2018

J Hellenic Vet Med Soc

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Cryopreservation negatively affects equine sperm quality post-thaw: an excessive release of reactive oxygen species (ROS) and increased protein tyrosine phosphorylation (known as cryocapacitation) have been demonstrated in frozenthawed equine sperm diminishing its lifespan. The objective of this study was to determine the possible beneficial effect of genistein addition (a ROS scavenger and protein tyrosine kinase inhibitor) to a commercial freezing extender. Equine sperm were frozen in the presence of different genistein concentrations ranging from 0 to 800 μM. After thawing, the sperm viability (eosinnigrosin), acrosome integrity using peanut agglutinin (PNA), protein tyrosine phosphorylation (PY) and motility were assessed at times 0 and 1 hr. In addition PY was studied in fresh and frozen sperm incubated in Modified Whitten’s (MW) medium with 25 mM Bicarbonate (MW+Bic). Genistein did not affect the viability, total or progressive motility or acrosome status of frozenthawed equine sperm. Immediately after thawing, positive PY staining in the equatorial band was observed and genistein addition did not exert any change in PY. Fresh sperm incubated in MW+Bic showed a significant increase in PY staining along the tail compared to frozen-thawed sperm. In our study sperm viability immediately post-thaw was 58.25% ± 5.35 and progressive motility 14.46% ± 5.7 (mean ± S.E.M.) and genistein did not improve the post-thaw quality of equine sperm. In addition, cryopreservation did not induce PY immediately post-thaw or enhanced frozen-thawed equine sperm susceptibility to PY induction.

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Addition of Glutathione to an Extender for Frozen Equine Semen

Cited by 44 publications

References 17 publications

Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations

Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations

Nutraceutical in male reproduction

Effect of genistein addition to equine sperm freezing extender

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