Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations

Oliveira, Rodrigo Arruda de; Viu, Marco Antônio de Oliveira; Gambarini, M. L.

doi:10.5433/1679-0359.2015v36n6p3699

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…Nevertheless, the reported inferior semen quality in the case of nontreated sperms and small doses of GSH and taurine might refect the damage to the sperm because of excessive ROS production that exceeds the normal antioxidant potency of sperms [41]. Te variations in results between diferent studies might be due to variations in the reduced levels of normal sperm antioxidant power [66], pH, and osmolality [67]. Besides the supplementation dose, species diferences exist in withstanding cryopreservation and cooling-associated stress and thawing-freezing problems [68].…”

Section: Discussionmentioning

confidence: 97%

Extender Supplementation with Glutathione (GSH) and Taurine Improves In Vitro Sperm Quality and Antioxidant Status of New Zealand Rabbits during Chilled Storage for up to 72 hours

Bayomy,

Hassab El-Nabi,

El Kassas

et al. 2023

Veterinary Medicine International

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This study assessed the influence of supplementing the rabbit semen extender with various concentrations of glutathione (GSH) and taurine at 24, 48, and 72 h postchilling at 5°C. Semen samples were collected from 20 New Zealand bucks, and ejaculates with standard color, motility (>85%), about 0.5 mL volume, and ∼400 × 106/mL concentration were used and diluted with extenders supplemented with 0.5, 1, and 2 mM of GSH and 1, 5, and 10 mM of taurine and chilled at 5°C. Nonsupplemented samples were used as a control. Sperm’s progressive motility, acrosome reaction, and extracellular oxidative stress biomarkers such as MDA contents and GPx, SOD, and CAT concentrations and intracellular transcriptomic levels of SOD and CAT genes were assessed. GSH and taurine supplementation improved the sperm’s kinetics by reducing cooling-associated stress, which was ascertained by lowering MDA concentration and increasing SOD, CAT, and GPx concentrations ( P < 0.05). Increasing the levels of antioxidant enzymes in the extender was due to the increasing mRNA copies of the SOD and CAT genes ( P < 0.05). Furthermore, GSH and taurine maintained the fructose levels in the extender and lowered the GPT levels, which implies sperm membrane stability is maintained through GSH and taurine supplementation. GSH and taurine supplementation to the extender had protective influences on the in vitro rabbit semen quality during chilled storage for up to 72 h, which were remarkable with increasing supplementation dose and cooling time at 5°C.

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Section: Discussionmentioning

confidence: 97%

Extender Supplementation with Glutathione (GSH) and Taurine Improves In Vitro Sperm Quality and Antioxidant Status of New Zealand Rabbits during Chilled Storage for up to 72 hours

Bayomy,

Hassab El-Nabi,

El Kassas

et al. 2023

Veterinary Medicine International

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Seminal Plasma Does Not Influence Canine Semen Stored at 5°C for Long-Term Conservation

Araujo

Paulo

Paulini

et al. 2022

Biopreservation and Biobanking

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Impact of supplementation of semen extender with antioxidants on quality of cooled or cryopreserved Marwari stallion spermatozoa

Kumar

Mehta

et al. 2021

Indian J of Anim Sci

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The aim of the present study was to evaluate the effects of supplementation of semen extender with two antioxidants namely Ascorbic acid (AA @ 0.9 g/L), Glutathione (GSH @ 2.5 mM), and combination of both (AA @ 0.9 g/L + GSH @ 2.5 mM) either in alone or in combination on the quality of cooled or cryopreserved Marwari stallion spermatozoa. For this purpose, a total of 24 ejaculates were collected from four adult and fertile Marwari stallions (6 ejaculates from each stallion) using an artificial vagina. Each freshly ejaculated semen sample was investigated for the semen quality parameters, viz. colour, consistency, total volume, gel volume, gel free volume, pH, progressive sperm motility, sperm concentration, sperm viability, sperm plasma membrane integrity, acrosomal integrity and DNA integrity. In the freshly ejaculated semen, no significant variation was found among individual stallions for various semen quality parameters except in sperm concentration. Pre-freeze and post-thaw semen evaluation revealed that the values for the most of the semen quality parameters were significantly higher in the semen extender being treated with the combination (AA @ 0.9 g/L +GSH @ 2.5 mM) of antioxidants group rather than AA and GSH alone or control. Addition of AA (0.9 g/L) and GSH (2.5 mM) to the freezing extender improved equine pre-freeze and post-thaw semen quality with the superiority of control group which indicates the beneficial role of supplementation of antioxidants to the stallion semen during cryopreservation process.

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Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations

Cited by 3 publications

References 10 publications

Extender Supplementation with Glutathione (GSH) and Taurine Improves In Vitro Sperm Quality and Antioxidant Status of New Zealand Rabbits during Chilled Storage for up to 72 hours

Extender Supplementation with Glutathione (GSH) and Taurine Improves In Vitro Sperm Quality and Antioxidant Status of New Zealand Rabbits during Chilled Storage for up to 72 hours

Seminal Plasma Does Not Influence Canine Semen Stored at 5°C for Long-Term Conservation

Impact of supplementation of semen extender with antioxidants on quality of cooled or cryopreserved Marwari stallion spermatozoa

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