Adaptive Significance of a Small Heat Shock/α-Crystallin Protein (p26) in Encysted Embryos of the Brine Shrimp,<i>Artemia franciscana</i>

Clegg, James S.; Willsie, Julia K.; Jackson, Susan A.

doi:10.1093/icb/39.6.836

Cited by 71 publications

(66 citation statements)

References 40 publications

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“…Induction of small Hsps in C. finmarchicus was not surprising, as they play a direct role in stress tolerance in arthropods (Clegg et al, 1999;MacRae, 2010;. The cytosolic Hsp70 subfamily includes a mixture of constitutively expressed (cognate) and inducible forms (Daugaard et al, 2007), with the inducible genes varying among taxa (e.g., in mammals the cognate and inducible forms of cytosolic Hsp70 resulted from lineage-specific duplication and subsequent diversification).…”

Section: Discussionmentioning

confidence: 99%

“…Production of Hsp70 and Hsp90 transcripts in C. finmarchicus has been previously examined in response to stressors such as increased temperature and exposure to toxins (Hansen et al, 2007;Hansen et al, 2008;Voznesensky et al, 2004), but not as a factor regulating diapause. Small Hsps have been shown to play an important role in stress tolerance during diapause in the crustacean Artemia franciscana (Clegg et al, 1999;, but have yet to be investigated in calanoid copepods. In this study, we examined the expression patterns of several large and small Hsps (Hsp90, 4 forms of Hsp70, Hsp21,Hsp22,p26) in individual diapausing and active C. finmarchicus C5 copepodids by quantitative real-time PCR (qRT-PCR).…”

Section: Introductionmentioning

confidence: 99%

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Heat shock protein expression during stress and diapause in the marine copepod Calanus finmarchicus

Aruda

Baumgartner

Reitzel

et al. 2011

Journal of Insect Physiology

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Graphical Abstract Research HighlightsWe identified 8 heat shock proteins (3 small Hsps, 4 Hsp70's, Hsp90). Hsp70a (cytosolic clade) and Hsp21, and Hsp22 were induced by handling stress. Hsp70a expression was elevated in shallow, active animals. Hsp22 expression was elevated in deep, diapausing animals. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 *Research highlights

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Heat shock protein expression during stress and diapause in the marine copepod Calanus finmarchicus

Aruda

Baumgartner

Reitzel

et al. 2011

Journal of Insect Physiology

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“…In addition, the companion study of Tomanek and Zuzow (Tomanek and Zuzow, 2010) found that M. galloprovincialis and M. trossulus showed different responses to heat stress in the expression of four small Hsp isoforms. Homologs of Hsp24 belong to the alpha-crystallin family of small Hsps , and have been shown to be important chaperones in the stress responses of a diverse array of animals, including humans (Horwitz, 1992), brine shrimp Artemia franciscana (Clegg et al, 1999), the annual killifish Austrofundulus limnaeus (Podrabsky and Somero, 2004) and the mussel M. californianus (Gracey et al, 2008). Small Hsps have been shown to inhibit apoptosis (Beere, 2004) and to increase resistance to oxidative stress in mammalian cells (Lee et al, 2004).…”

Section: Cytoskeletal Protection: a Potential Mechanism For Increasedmentioning

confidence: 99%

Transcriptomic responses to heat stress in invasive and native blue mussels (genus Mytilus): molecular correlates of invasive success

Lockwood

Sanders

Somero

2010

Journal of Experimental Biology

225

279

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SUMMARYInvasive species are increasingly prevalent in marine ecosystems worldwide. Although many studies have examined the ecological effects of invasives, little is known about the physiological mechanisms that might contribute to invasive success. The mussel Mytilus galloprovincialis, a native of the Mediterranean Sea, is a successful invader on the central and southern coasts of California, where it has largely displaced the native congener, Mytilus trossulus. It has been previously shown that thermal responses of several physiological traits may underlie the capacity of M. galloprovincialis to out-compete M. trossulus in warm habitats. To elucidate possible differences in stress-induced gene expression between these congeners, we developed an oligonucleotide microarray with 8874 probes representing 4488 different genes that recognized mRNAs of both species. In acute heat-stress experiments, 1531 of these genes showed temperature-dependent changes in expression that were highly similar in the two congeners. By contrast, 96 genes showed species-specific responses to heat stress, functionally characterized by their involvement in oxidative stress, proteolysis, energy metabolism, ion transport, cell signaling and cytoskeletal reorganization. The gene that showed the biggest difference between the species was the gene for the molecular chaperone small heat shock protein 24, which was highly induced in M. galloprovincialis and showed only a small change in M. trossulus. These different responses to acute heat stress may help to explain -and predict -the invasive success of M. galloprovincialis in a warming world. Supplementary material available online at

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“…In D. magna, a common heat-shock protein, Hsp60, is constitutively present but also can be induced by predator stress (Pijanowska & Kloc, 2004;Pauwels et al, 2005). There are strong data indicating that heat-shock proteins are an important mechanism to maintain protein structural integrity, and to inhibit DNA synthesis and cell division during diapause in large branchiopods (Artemia fransicana; Clegg et al, 1999) and insects (Denlinger, 2002).…”

Section: Introductionmentioning

confidence: 99%

Biochemical adaptation for dormancy in subitaneous and dormant eggs of Daphnia magna

et al. 2007

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Daphnia can reproduce through subitaneous and dormant eggs. The production of dormant eggs is induced by stimuli associated with deteriorating growth conditions, and enable Daphnia populations to survive temporarily harsh environmental conditions. Dormant eggs are expected to have developed special biochemical adaptations to bridge this long unfavourable period, but little comparative biochemical data are available for dormant and subitaneous eggs. We compared levels of the following molecules between subitaneous and dormant eggs: (a) triglycerides, which are the most abundant energy storage molecules in Daphnia, (b) glycerol, a cryoprotectant also involved in energy storage, and (c) the heat shock protein Hsp60, a molecular chaperone that may assist in maintaining protein structural integrity and inhibiting cell metabolism during diapause. Unexpectedly, no difference in triglycerides content between egg types was found. As expected, dormant eggs contained more glycerol and relatively more Hsp60 than subitaneous eggs. The biochemical composition of dormant eggs can therefore be seen as an adaptation to the harsh environmental conditions these eggs encounter.

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Adaptive Significance of a Small Heat Shock/α-Crystallin Protein (p26) in Encysted Embryos of the Brine Shrimp,Artemia franciscana

Cited by 71 publications

References 40 publications

Heat shock protein expression during stress and diapause in the marine copepod Calanus finmarchicus

Heat shock protein expression during stress and diapause in the marine copepod Calanus finmarchicus

Transcriptomic responses to heat stress in invasive and native blue mussels (genus Mytilus): molecular correlates of invasive success

Biochemical adaptation for dormancy in subitaneous and dormant eggs of Daphnia magna

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