Adaptation of the Sensititre [Registered Trademark] Broth Microdilution Technique to Antimicrobial Susceptibility Testing of Mycoplasma gallisepticum

Tanner, A. C.; Wu, Ching Ching

doi:10.2307/1591770

Cited by 42 publications

(30 citation statements)

References 6 publications

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“…Examples of these methods are the liquid method described by Tanner and Wu [39], originally designed to test strains of M. gallisepticum and the solid agar method described by Hannan et al [15]. Both of these methods are also applicable to testing mycoplasmas of human origin and have been used to test a variety of different mycoplasma species from various animal hosts.…”

Section: Mycoplasma Mic Methodsmentioning

confidence: 99%

Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary mycoplasma species

Hannan¹

2000

Vet. Res.

281

290

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-The absence of standardised procedures for minimum inhibitory concentration (MIC) testing of antimicrobial agents against veterinary mycoplasma and ureaplasma species (Mollicutes) has made it difficult to compare results originating from different laboratories. This report, prepared on behalf of the International Research Programme on Comparative Mycoplasmology (IRPCM), offers guidelines and recommendations for veterinary MIC testing of these organisms in an effort to rectify this problem. The subjects discussed include suitable media for broth and agar MIC assays, storage and preparation of antimicrobial agents, standardisation of mycoplasma inocula for MIC tests, validation of equipment, incubation conditions, and determination of MIC end points. A standard medium for all veterinary mycoplasma MIC tests cannot currently be recommended, owing to the diversity of nutritional requirements of different mycoplasma species. Instead mycoplasma broths or agars giving optimal growth of specific mycoplasmas or ureaplasmas are recommended, as suboptimal growth may lead to falsely low MIC results. The importance of using standardised mycoplasma inocula, for assays using either solid or liquid media is stressed. The growth phase may be less important as lag phase and logarithmic phase cultures of Mycoplasma gallisepticum, M. synoviae, M. bovis and M. hyopneumoniae have given very similar results in liquid MIC assays. The liquid method of Tanner and Wu and the agar method described by Hannan et al. are compared and described in detail. Methods for calculating MIC 50 s and MIC 90 s are described and the interpretation of results discussed. Methods for assessing mycoplasmacidal (MMC) activity of antimicrobial agents are also described. Adoption of these guidelines should lead to more consistent MIC results being obtained between laboratories. MIC

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Section: Mycoplasma Mic Methodsmentioning

confidence: 99%

Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary mycoplasma species

Hannan¹

2000

Vet. Res.

281

290

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“…MIC tests were performed in 96-well, microdilution sensititre plates by a modification of the method described by Tanner and Wu [30]. The compound dilutions were mixed with an equal volume (100 pl) of the challenge inocula containing 1 O3 -1 O5 ccu/ml in microtitration wells.…”

Section: Mic Testsmentioning

confidence: 99%

Comparative Susceptibilities of Various Aids-Associated and Human Urogenital Tract Mycoplasmas and Strains of Mycoplasma Pneumoniae to 10 Classes of Antimicrobial Agent In Vitro

Hannan¹

1998

Journal of Medical Microbiology

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The susceptibilities of 40 strains of various Mycoplasma species to 10 classes of antimicrobial agents were compared in vitro by a broth microdilution method. The strains tested comprised 20 strains of four AIDS-associated species -M. penetrans (1 strain), M. fermentas (5 strains), M. pirum (6 strains) and M. genitalium (8 strains) -nine strains of the urogenital tract species M. hominis and 11 strains of M. pneumoniae. The results demonstrated wide variation in the susceptibilities of the different Mycoplasma spp. to different classes of antimicrobial agent. All the mycoplasmas were susceptible or highly susceptible to the fluoroquinolones, with sparfloxacin the most active, and to the diterpine antibiotic tiamulin. M. pneumoniae and M. genitnlium strains were also highly susceptible to the macrolides, particularly azithromycin and had similar antibiotic susceptibility patterns to most other antimicrobial agents. However, all strains of M. genitalium were resistant to streptomycin (MIC 250->500 mg/L) whereas all M. pneumoniae isolates, except the MAC strain, were susceptible (MICs 1.25-12.5 mg/L). M. pirum isolates varied considerably in their susceptibility to macrolides (MIC range versus azithromycin 0.0025-> 100 mg/L). M. .fermentarts strains were susceptible to the tetracyclines, lincosamides and mupirocin, but varied in susceptibility to aminoglycosides. Most M. hominis strains were susceptible to the tetracyclines and all were susceptible to clindamycin and mupirocin. M penetrans GTU 54 was susceptible to azithromycin, the tetracyclines and lincosamides as well as to the fluoroquinolones and tiamulin.

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“…Alun and Ching-Ching [6] and Pakpinyo and Sasipreeyajan [7] reported strong resistance to erythromycin, while Ellakany et al [8] found that MG field isolates from Egypt were highly sensitive to tiamulin, tylosin, but moderately sensitive to enrofloxacin. However, it was reported in literature that resistance to tylosin increased in the last decades [9,10].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antibiotics to Control Mycoplasma gallisepticum in Broiler Breeder Chickens

Farran¹,

Ellakany²,

Shaib³

et al. 2018

Poult Fish Wildl Sci

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This study aims at the evaluation of the efficacy of Pulmotil and Denagard in comparison to the generic tylosin against Mycoplasma gallisepticum (MG) infection in broiler breeders. A total of 600 twenty four-week old broiler breeder pullets along with their 60 roosters of the Ross 308 strain, were equally subdivided into four treatments of 150 birds each with 3 replicates per treatment (50 pullets and 5 roosters/pen). The MG-free birds of Group 1, the Control, were kept in a separate house and left with no MG-challenge or drug administration while those of groups 2, 3, and 4 were raised in another house and previously challenged with MG at 2 and 24 weeks of age respectively. Every 4 weeks and for 3 consecutive days, birds of group 3 were administered Pulmotil (3-21 Weeks) and Denagard (25-44 weeks), whereas those of Group 4 were treated throughout the trial with generic Tylosin. Production performance parameters were not significantly different among the differently treated birds. At 30 weeks of age, Pulmotil-Denagard treatment significantly reduced the tracheal MG counts to a similar level to that of the controls and restored the fertility of the MG challenged birds at 39 weeks of age. At 35 weeks of age, Pulmotil-Denagard treatment significantly reduced the frequency of day-old progeny with positive airsac lesions. It also reduced MG colonization in the airsacs of the day-old offsprings of the 35 and 39 week old breeders. As the Pulmotil-Denagard treatment significantly reduced the MG colonization of bird tissues, it reduced MG sera titers at 12, 20 and 30 weeks of age, and increased titers against IB, IBD and ND at later stages. In conclusion, the use of Pulmotil/Denagard program is highly recommended in MG-infected breeder farms, and protects against any potential MG endemic infection.

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Adaptation of the Sensititre [Registered Trademark] Broth Microdilution Technique to Antimicrobial Susceptibility Testing of Mycoplasma gallisepticum

Cited by 42 publications

References 6 publications

Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary mycoplasma species

Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary mycoplasma species

Comparative Susceptibilities of Various Aids-Associated and Human Urogenital Tract Mycoplasmas and Strains of Mycoplasma Pneumoniae to 10 Classes of Antimicrobial Agent In Vitro

Evaluation of Antibiotics to Control Mycoplasma gallisepticum in Broiler Breeder Chickens

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