Adaptation of DPPH Method for Antioxidant Determination

Abstract

Antioxidants are indispensable molecules that help prevent biomolecule oxidation. By fighting the production of free radicals, antioxidants prevent different ageing-associated diseases. In this work, the method of 2,2-diphenyl-2-picrilhydracil (DPPH) is adapted to determine antioxidants in different cosmetic, nutritional, and biological samples using spectrophotometric and electrochemical techniques. Antioxidant molecules of Trolox (Tx) and gallic acid are used as a standard, whereas spectroscopic and electrochemical properties of DPPH free radical are used to determine total antioxidant capacity (meq Tx/mL) in different commercial products and biological samples. Oxidation-reduction properties of DPPH at a carbon glass electrode allow applying cyclic voltammetry and chronoamperometry to construct amperometric calibration curves

Electrochemical Profiling of Plants

“…The concept can be further extended to the subcellular analysis of plants, with outstanding data enabling further manipulation of the essential constraints for improving health and product outcomes using plants. Leon et al compared electrochemical and conventional techniques for the determination of antioxidants used in the monitoring of DPPH radical capture [108]. On comparison between spectrophotometric and chronoamperometry techniques, sample dilution and pretreatments steps were easily eliminated in cases of amperometry without any issues of concentration and absorbance uncertainty.…”