Acute symptomatic seizures following intracerebral hemorrhage in the rat collagenase model

Germonpré, Charlotte; Proesmans, Silke; Bouckaert, Charlotte; Stevens, Latoya; Sprengers, Mathieu; Vonck, Kristl; Carrette, Evelien; Wadman, Wytse J.; Boon, Paul; Raedt, Robrecht; Herdt, Veerle De

doi:10.1016/j.eplepsyres.2020.106364

Cited by 5 publications

(12 citation statements)

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“…Acute symptomatic seizures were present in 33% of the animals, which is similar to our previous study in the ICH rat collagenase model where we found an incidence of acute seizures of 45% (Germonpré et al, 2020). This incidence is higher compared to the reported incidence of 8-14% acute symptomatic seizures post-ICH in the human situation (De Herdt et al, 2011;Haapaniemi et al, 2014;de Greef et al, 2015).…”

Section: Discussionsupporting

confidence: 87%

“…Continuous video-EEG monitoring (VEM) was performed as previously described (Germonpré et al, 2020). Briefly, the EEG setup consisted of a custom-build head stage, carrying a 4channel unity gain amplifier, connected via tethers to a 12channel commutator (Plastics One, Roanoke, VA, United States), allowing the animals to move freely, and a 512× amplifier.…”

Section: Video-eeg Monitoringmentioning

confidence: 99%

“…If a seizure was detected on the EEG, the corresponding videofiles were used to check the clinical symptoms of the seizure or to exclude non-epileptic activity. The clinical seizures were scored according to the modified Racine Scale (Racine, 1972;Germonpré et al, 2020): (1) wet dog shakes; (2) head nodding, facial clonus, eye blinking, disoriented walk with curved back;…”

Section: Video-eeg Monitoringmentioning

confidence: 99%

“…We confirmed this in our previous study and additionally performed video-EEG to characterize the behavioral aspects of the seizures. We found that 45% of rats injected with collagenase in the striatum display acute symptomatic seizures during the first 4 days after the bleeding (Germonpré et al, 2020). However, it is not known if late, unprovoked seizures occur in the rat collagenase model as long-term video-EEG monitoring beyond 30 days post-ICH has not been performed.…”

Section: Introductionmentioning

confidence: 96%

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Seizures and Interictal Epileptiform Activity in the Rat Collagenase Model for Intracerebral Hemorrhage

et al. 2021

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AimsIntracerebral hemorrhage (ICH) is a known risk factor for the development of acute symptomatic as well as late unprovoked seizures. The underlying pathophysiology of post-ICH seizures is incompletely understood and there are no reliable predictive biomarkers. An animal model to study post-ICH seizures is currently lacking. The aim of this study was to investigate (1) the occurrence of seizures and interictal epileptiform activity in the ICH rat collagenase model using long-term video-EEG monitoring (VEM) and (2) whether seizure occurrence was associated with interictal epileptiform activity and histological features.MethodsMale Sprague-Dawley rats were implanted with epidural electrodes. After 1 week of baseline VEM, collagenase was injected in left striatum to induce an ICH. VEM was continued for 180 days to assess the occurrence of post-ICH seizures and interictal epileptiform activity (spikes and epileptiform discharges). At the end of the experiment, animals were euthanized for histological characterization of the hemorrhagic lesion, using cresyl violet, Prussian blue and immunofluorescence staining.ResultsAcute symptomatic seizures occurred in 4/12 animals between 46 and 80 h after ICH induction. Late unprovoked seizures were present in 2/12 animals and started at 90 and 103 days post-ICH. Animals with late unprovoked seizures did not have acute symptomatic seizures. All electrographic seizures were accompanied by clear behavioral changes. Interictal spikes and epileptiform discharges were observed in all animals but occurred more frequently in rats with late seizures (p = 0.019 and p < 0.001, respectively). Animals with acute symptomatic seizures had more extended hemorrhagic lesions and hemosiderin deposits in the piriform cortex.ConclusionBoth acute symptomatic and late unprovoked seizures were observed in the rat collagenase model. Interictal epileptiform activity was more frequently seen in animals with late seizures. Rats with acute symptomatic seizures showed more extensive lesions and hemosiderin deposits in the piriform cortex. This model could be used to further explore possible biomarkers for epileptogenesis.

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Section: Discussionsupporting

confidence: 87%

Section: Video-eeg Monitoringmentioning

confidence: 99%

Section: Video-eeg Monitoringmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Seizures and Interictal Epileptiform Activity in the Rat Collagenase Model for Intracerebral Hemorrhage

et al. 2021

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“…Continuous video-EEG monitoring was performed as described previously by Germonpré et al and is explained here in short [ 28 ]. After electrode implantation surgery, animals were housed in neurophysiology cages (Plexiglas 29 × 29 × 45 cm) where they were connected to a video-EEG monitoring setup.…”

Section: Methodsmentioning

confidence: 99%

Development of a Rat Model for Glioma-Related Epilepsy

Bouckaert

Germonpré

Verhoeven

et al. 2020

IJMS

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Seizures are common in patients with high-grade gliomas (30–60%) and approximately 15–30% of glioblastoma (GB) patients develop drug-resistant epilepsy. Reliable animal models are needed to develop adequate treatments for glioma-related epilepsy. Therefore, fifteen rats were inoculated with F98 GB cells (GB group) and four rats with vehicle only (control group) in the right entorhinal cortex. MRI was performed to visualize tumor presence. A subset of seven GB and two control rats were implanted with recording electrodes to determine the occurrence of epileptic seizures with video-EEG recording over multiple days. In a subset of rats, tumor size and expression of tumor markers were investigated with histology or mRNA in situ hybridization. Tumors were visible on MRI six days post-inoculation. Time-dependent changes in tumor morphology and size were visible on MRI. Epileptic seizures were detected in all GB rats monitored with video-EEG. Twenty-one days after inoculation, rats were euthanized based on signs of discomfort and pain. This study describes, for the first time, reproducible tumor growth and spontaneous seizures upon inoculation of F98 cells in the rat entorhinal cortex. The development of this new model of GB-related epilepsy may be valuable to design new therapies against tumor growth and associated epileptic seizures.

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