Acute aerocystitis in Piaractus mesopotamicus: Participation of eicosanoids and pro-inflammatory cytokines

Claudiano, Gustavo da Silva; Petrillo, Thalita Regina; Manrique, Wilson Gómez; Castro, Marcello Pardi de; Loureiro, Bruna Agy; Marcusso, Paulo Fernandes; Belo, Marco Antônio de Andrade; Moraes, Julieta Rodini Engrácia de; Moraes, Flávio Ruas de

doi:10.1016/j.fsi.2013.01.006

Cited by 29 publications

(26 citation statements)

References 26 publications

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“…Belo et al (2005) found that Piaractus mesopotamicus with high serum cortisol levelsstressed fish -presented lesser MGCs, due likely to the anti-inflammatory action of this corticosteroid in the initial phase of the process. This response is due to the inhibition of the formation of eicosanoids and pro-inflammatory cytokines, blocking chemotaxis (CLAUDIANO et al 2013). Dexamethasone in conjunction with cortisol -from handling stress-may inhibit the action of IL-4 and IL-13: important cytokines to macrophages fusion (BRODBECK; ANDERSON, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…Finally, intramuscular injections of dexamethasone and/or levamisole were applied according Table 1. Dexamethasone dose (2.0 mg/kg) used in this experiment was previously tested in this species in our laboratory (CLAUDIANO et al, 2013). To determinate the dose of levamisole it was performed an essay with four doses (25, 50, 100, and 200 mg of levamisole/kg of fish) and was evaluated the total number of leukocytes in blood.…”

Section: Methodsmentioning

confidence: 99%

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Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus

Petrillo¹,

Claudiano²,

Yunis‐Aguinaga³

et al. 2017

Biosci. J.

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ABSTRACT:The aim of this study was to evaluate the effect of parenteral administration of dexamethasone and levamisole on the accumulation of macrophages and formation of giant cells in chronic inflammation by foreign body and blood parameters in pacu (Piaractus mesopotamicus). It was used 50 mg/kg of levamisole and 2.0 mg/kg of dexamethasone and the combination of both drugs. Coverslips were implanted under the skin. After 2, 7, and 15 days postimplantation (DPI), fish were anesthetized for removal of coverslips and the number of macrophages and giant cells were count. It was also collected blood from the caudal vessel to evaluate: red blood cell count, hematocrit, hemoglobin concentration, leukocytes count, thrombocytes count, MCV, and MCHC. We observed that dexamethasone affects negatively the formation of giant cells in the chronic inflammation for foreign body. Levamisole, despite being immunostimulatory in several species, showed limited action. However, it was enough to counteract the effect of dexamethasone; the association of the drugs did not interfere significantly in erythrocyte and leukocyte number in most of the treatments and times studied. In dexamethasone group there was a reduction in the number of erythrocytes and hemoglobin associated with increased mean corpuscular volume, suggesting slight macrocytic anemia. At 15 DPI, most groups showed the recovery of hematologic response. As in mammals, dexamethasone affects negatively the inflammatory response. Levamisole showed little effect by itself. However, in some parameters the association of both drugs causes similar response to control and naïve groups, showing the antagonistic effect of these drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus

Petrillo¹,

Claudiano²,

Yunis‐Aguinaga³

et al. 2017

Biosci. J.

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“…Quando ocorre a invasão de microrganismos, o sistema imune inicia a ativação e a proliferação de leucócitos e trombócitos (Kollner et al, 2002), os quais estão envolvidos na defesa do organismo (Bozzo et al, 2007;Claudiano et al, 2013).…”

Section: Resultsunclassified

Desempenho e hematologia de tilápias-do-nilo alimentadas com Saccharomyces cerevisiae e vacinadas contra Streptococcus agalactiae

Salvador

Claudiano

Loureiro³

et al. 2013

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a inter-relação entre a suplementação alimentar com parede celular de Saccharomyces cerevisae e a vacinação contra Streptococcus agalactiae e seu efeito sobre o desempenho produtivo e as variáveis hematológicas de tilápia-do-nilo (Oreochromis niloticus). Oitenta e quatro tilápias-do-nilo foram distribuídas em 12 caixas de fibra (n=7), em arranjo fatorial 2x2x3, correspondente a dois níveis de suplementação com parede celular de levedura, dois tipos de inoculação e três tempos de avaliação. Os peixes foram alimentados durante 77 dias. A vacinação dos peixes foi realizada 60 dias após o início da alimentação. Quinze dias após a vacinação, todos os peixes foram submetidos ao desafio com cepa viva de S. agalactiae, e 6, 24 e 48 horas após o desafio, o sangue foi colhido da veia caudal para avaliações. Peixes alimentados com ração suplementada apresentam maior ganho de peso e taxa de crescimento específico, e a interação entre os efeitos da dieta e da vacinação resulta em maiores taxas de hematócrito, hemoglobina e leucócitos.Termos para indexação: Oreochromis niloticus, inflamação, probiótico, sangue, teleósteos, vacinação. Performance and hematological profile of Nile tilapia fed with Saccharomyces cerevisiae and vaccinated against Streptococcus agalactiaeAbstract -The objective of this work was to evaluate the inter-relationship between dietary supplementation with Saccharomyces cerevisiae cell wall and vaccination against Streptococcus agalactiae, and its effect on the productive performance and hematological variables of Nile tilapia (Oreochromis niloticus). Eighty-four Nile tilapia were distributed in 12 fiber boxes (n=7), in a 2x2x3 factorial arrangement, corresponding to two levels of supplementation with yeast cell wall, two types of inoculation, and three evaluation times. Fish were fed during 77 days. Vaccination of fish was done 60 days after feeding started. Fifteen days after vaccination, all fish were subjected to challenge with live strain of S. agalactiae, and 6, 24, and 48 hours after the challenge, blood was collected from the caudal vein for evaluations. Fish fed with supplemented diets show greater weight gain and specific growth rate, and the interaction between the diet and vaccination effects results in higher hematocrit, hemoglobin, and leukocyte rates.

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“…Farmed fish are in constant contact with different agents capable of provoking inflammatory condition (MARTINS et al, 2001) Inflammatory response has been evaluated under several factors such as fish fed supplemented diets containing vitamins (BELO et al, 2005;MARTINS et al, 2008a;BELO et al, 2012), probiotics (REQUE et al, 2010;DOTTA et al, 2011;CASTRO et al, 2014a), amino acids, minerals and polysaccharides (FALCO et al, 2012;CASTRO et al, 2014b;MACHADO et al, 2015), phytotherapics (DOTTA et al, 2015), stocking density (BELO et al, 2005) and the stressful effect on inflammatory response (MARTINS et al, 2004). Some phlogogen agents have been used to stimulate cell migration and the most used are carrageenin (MATUSHIMA; MARIANO, 1996;MARTINS et al, 2001;MARTINS et al, 2004;MARTINS et al, 2008a;MARTINS et al, 2009;DOTTA et al, 2011), thioglycolate (MARTINS et al, 2001;BOZZO et al, 2007;MARTINS et al, 2009;MORAES et al, 2012), lipopolysaccharides (LPS) (MARTINS et al, 2004;BOZZO et al, 2007;MARTINS et al, 2008a;MORAES et al, 2012), inactivated bacteria (BOZZO et al, 2007;REQUE et al, 2010;MORAES et al, 2012;CLAUDIANO et al, 2013;CASTRO et al, 2014b;MACHADO et al, 2015) and glass coverslip (BELO et al, 2005;BELO et al, 2012). In Brazil, the inflammatory process in fish was studied in pacu Piaractus mesopotamicus …”

Section: Introductionmentioning

confidence: 99%

“…Some phlogogen agents have been used to stimulate cell migration and the most used are carrageenin (MATUSHIMA; MARIANO, 1996;MARTINS et al, 2001;MARTINS et al, 2004;MARTINS et al, 2008a;MARTINS et al, 2009;DOTTA et al, 2011), thioglycolate (MARTINS et al, 2001;BOZZO et al, 2007;MARTINS et al, 2009;MORAES et al, 2012), lipopolysaccharides (LPS) (MARTINS et al, 2004;BOZZO et al, 2007;MARTINS et al, 2008a;MORAES et al, 2012), inactivated bacteria (BOZZO et al, 2007;REQUE et al, 2010;MORAES et al, 2012;CLAUDIANO et al, 2013;CASTRO et al, 2014b;MACHADO et al, 2015) and glass coverslip (BELO et al, 2005;BELO et al, 2012). In Brazil, the inflammatory process in fish was studied in pacu Piaractus mesopotamicus (BELO et al, 2005;BOZZO et al, 2007;GARCIA et al, 2007;MORAES, 2009;BELO et al, 2012;CLAUDIANO et al, 2013;MORAES et al, 2012;CASTRO et al, 2014b), Nile tilapia Oreochromis niloticus (MATUSHIMA; MARIANO, 1996;MARTINS et al, 2004;MARTINS et al, 2008a;REQUE et al, 2010;DOTTA et al, 2011) and in the hybrid tambacu (P. mesopotamicus male x Colossoma macropomum female) (MARTINS et al, 2001;MARTINS et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Induced aerocystitis and hemato-immunological parameters in Nile tilapia fed supplemented diet with essential oil of <i>Lippia alba</i>

Rodrigues-Soares

Jesus

Gonçalves

et al. 2018

Braz. J. Vet. Res. Anim. Sci.

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This study evaluated the dietary supplementation with essential oil of Lippia alba on the hemato-immunological parameters of Nile tilapia (Oreochromis niloticus) submitted to acute inflammation induced by carrageenin injection in the swim bladder. For a period of 45 days, 96 fish were divided into four treatments in triplicate, as follows: (a) fish fed supplemented diet with essential oil of L. alba (4 mL kg -1 dry ration) injected with carrageenin; (b) fish fed supplemented diet with cereal alcohol injected with carrageenin; (c) fish fed unsupplemented diet with essential oil injected with carrageenin; (d) fish fed unsupplemented diet and noninjected. Cortisol levels, erythrogram, leukogram and the inflammatory infiltrate were analyzed 6 hours after an inflammatory stimulus. Carrageenin-injected fish showed an acute inflammatory reaction in the swim bladder characterized by higher infiltrate of neutrophils and monocytes. The circulating neutrophils number was significantly higher in fish fed L. alba when compared to other treatments. No difference in cortisol levels was found. For the dose, time and administration form tested, supplementation with essential oil of L. alba did not present anti-inflammatory activity. On the other hand, an influence of dietary supplementation was observed on the neutrophils number after induced aerocystitis highlighting its immunomodulatory characteristic. Keywords: Oreochromis niloticus. Stress. Phytotherapics. Hematology. Acute inflammation. ResumoEste estudo avaliou a suplementação dietária com óleo essencial de Lippia alba sobre os parâmetros hematoimunológicos em tilápias do Nilo (Oreochromis niloticus) submetidas à inflamação aguda induzida por carragenina na bexiga natatória. Pelo período de 45 dias, 96 peixes foram divididos em quatro tratamentos em triplicata: (a) peixes suplementados com óleo essencial de L. alba (4 mL kg -1 de ração seca) injetados com carragenina; (b) peixes suplementados com álcool de cereais injetados com carragenina; (c) peixes não suplementados com óleo essencial injetados com carragenina; (d) peixes não suplementados e não injetados. Os níveis de cortisol, o eritrograma, o leucograma e o infiltrado inflamatório foram analisados seis horas após o estímulo inflamatório. Peixes injetados com carragenina apresentaram reação inflamatória aguda na bexiga natatória caracterizada pela maior concentração de infiltrado de neutrófilos e monócitos. O número de neutrófilos circulantes foi significativamente maior nos peixes suplementados com L. alba, numa comparação com os outros tratamentos. Não houve diferença nos níveis de cortisol. Para a dose, o tempo e a forma de administração testada, a suplementação com óleo essencial de L. alba não apresentou atividade anti-inflamatória. Por outro lado, foi constatada influência da suplementação dietária no número de neutrófilos após a aerocistite, enfatizando a sua característica imunomoduladora. Palavras-chave: Oreochromis niloticus. Estresse. Fitoterápicos. Hematologia. Inflamação aguda.

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Acute aerocystitis in Piaractus mesopotamicus: Participation of eicosanoids and pro-inflammatory cytokines

Cited by 29 publications

References 26 publications

Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus

Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus

Desempenho e hematologia de tilápias-do-nilo alimentadas com Saccharomyces cerevisiae e vacinadas contra Streptococcus agalactiae

Induced aerocystitis and hemato-immunological parameters in Nile tilapia fed supplemented diet with essential oil of <i>Lippia alba</i>

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