Actual status of mutagenicity testing with the host-mediated assay

Mohn, G.R.

doi:10.1007/bf00293669

Cited by 19 publications

(1 citation statement)

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“…Ascites tumour cells and lymphoma cells of murine origin are being evaluated currently in place of bacterial and yeast indicator strains. Pres-ently, several laboratories are using mouse lymphoma cells in the host-mediated assay [35]. This remains an exceptionally difficult technique.…”

Section: Methodological Backgroundmentioning

confidence: 99%

The role of cytogenetic tests in detection and prevention of cancer

Bishun

1981

Journal of Surgical Oncology

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Although simplified and improved techniques have increased at a fast rate in recent years, a great number of compounds released into our environment still remain untested. It has been estimated that between 80-90% of human cancer is a result of exposure to such compounds, and if by the application of short-term mutagenic tests, the use of many of these compounds can be severely restricted, an enormous impact can be made on the solution of human health problems. Batteries of mutagenic tests have established an empirical relationship between mutagenisis and carcinogenisis, and, in view of the cost in terms of time and money, short-term tests are playing an important role in first detecting, and second, eliminating potential hazards in our environment. The use of bacteria and other unicellular organisms in these assay systems has met with much criticism; due to the fact that the DNA materials affected do not directly relate to that of man. However, in conjunction with other tests, utilizing human and other mammalian cells, firm conclusions can be drawn regarding the potential hazards of certain chemicals. Recent advances in cytogenetic tests (e.g., banding chromosomes and sister chromatid exchange) have improved the sensitivity of chromosomal tests and, in so doing, have rendered them more usual in the selecting out process that can reduce substantially the mutagenic and carcinogenic hazards caused by chemicals and other deleterious agents in the environment.

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Section: Methodological Backgroundmentioning

confidence: 99%

The role of cytogenetic tests in detection and prevention of cancer

Bishun

1981

Journal of Surgical Oncology

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Mutagenesis and carcinogenesis testing in vitro

Casto¹

1981

Biotech & Bioengineering

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SummaryThe development and validation of short-term tests for carcinogens and mutagens are urgently needed if estimates of 7040% for environmentally induced cancer are valid. Several methods for in vitro testing have been described in microbial and mammalian cells. It is anticipated that a battery of tests will soon be available with a high degree of reproducibility and predictability for potential in vivo carcinogenesis. This review discusses those assays currently being implemented in mammalian cells including a brief description of the methodology, advantages, and disadvantages, and the correlation with known in vivo activity.

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