Activity or mass concentration of bone-specific alkaline phosphatase as a marker of bone formation

Avberšek-Lužnik, Ivica; Stopar, Tanja Gmeiner; Marc, Janja

doi:10.1515/cclm.2007.186

Cited by 25 publications

(16 citation statements)

References 20 publications

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“…As such, it is widely used as an differentiation marker associated with osteogenesis (Dimai et al 1998;Avbersek-Luznik et al 2007). In the present study, about 46% of the cells were capable of developing into ALP positive cells under normal inducing condition, without pretreatment with 5-azacytidine.…”

Section: Alkaline Phosphatase Assaymentioning

confidence: 99%

5-Azacytidine facilitates osteogenic gene expression and differentiation of mesenchymal stem cells by alteration in DNA methylation

Zhou

Zhang

et al. 2009

Cytotechnology

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Mesenchymal stem cells (MSCs) are considered to be one of the most promising therapeutic cell sources as they encompass a plasticity of multiple cell lineages. The challenge in using these cells lies in developing well-defined protocols for directing cellular differentiation to generate a desired lineage. In this study, we investigated the effect of 5-azacytidine, a DNA demethylating agent, on osteogenic differentiation of MSCs. The cells were exposed to 5-azacytidine in culture medium for 24 h prior to osteogenic induction. Osteogenic differentiation was determined by several the appearance of a number of osteogenesis characteristics, including gene expression, ALP activity, and calcium mineralization. Pretreatment of MSCs with 5-azacytidine significantly facilitated osteogenic differentiation and was accompanied by hypomethylation of genomic DNA and increased osteogenic gene expression. Taking dlx5 as a representative, methylation alterations of the "CpG island shore" in the promoter caused by 5-azacytidine appeared to contribute to osteogenic differentiation.

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Section: Alkaline Phosphatase Assaymentioning

confidence: 99%

5-Azacytidine facilitates osteogenic gene expression and differentiation of mesenchymal stem cells by alteration in DNA methylation

Zhou

Zhang

et al. 2009

Cytotechnology

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“…To evaluate the effect of distinct factors on bone metabolism, different bone markers have been identified [11]. The urinary cross-linked N-terminal telopeptides of type I collagen (NTX) is considered a specific bone resorption marker [2] and bone-alkaline phosphatase (ALP), is a recognized bone formation marker [12]. While exercise seems to be effective in lowering the risk of falls and maintain muscular performance in selected people and it should through alterations in regulation of bone metabolism prevent the deterioration [6].…”

Section: Introductionmentioning

confidence: 99%

The effects of combined training on bone metabolic markers in postmenopausal women

et al. 2016

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KEYWORDSExercise; Bone formation marker; Post-menopausal women SummaryObjectives. -Exercise plays an important role in maximizing and subsequent reduction of the maximum rates of bone loss. The purpose of this study was to determine whether 16 weeks of combined exercise in postmenopausal would affect bone metabolism. Results. -After the training period, postmenopausal significantly (P < 0.05) increase their muscle functionality (14% to 26.5%). Levels of NTX were not affected but there was a significant increase in bone synthesis (ALP: 12.5% to 15.9%), while there is no change in bone resorption. Combined training appears to be useful in improving total muscular performance and potentially in bone density. © 2016 Elsevier Masson SAS. All rights reserved. Résultats. -Après la période de formation, les femmes post-ménopausées ont vu la fonctionnalité des muscles augmenter significativement (p < 0,05) (14-26,5 %). Les niveaux de NTX n'ont pas été affectés, mais il y avait une augmentation significative de la synthèse osseuse (ALP : 12,5-15,9 %), alors qu'il n'y a aucun changement dans la réabsorption osseuse. La formation combinée semble être utile dans l'amélioration de la performance musculaire totale et, potentiellement, dans l'augmentation de la densité osseuse. MOTS CLÉS

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“…Bone alkaline phosphatase (b-ALP) is the enzyme isoform of total ALP synthesized by the osteoblast. The serum level of b-ALP is a sensitive bone formation marker to evaluate changes in estrogenic status and in monitoring the effect of bisphosphonate (BPs) treatment [6].…”

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confidence: 99%

Salivary bone turnover markers in healthy pre- and postmenopausal women: daily and seasonal rhythm

et al. 2011

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No studies had investigated circadian and circannual rhythms of bone biomarkers in whole saliva. We evaluated the salivary daily and seasonal rhythm of carboxy-terminal telopeptide of type I collagen (CTX) and bone alkaline phosphatase (b-ALP). Forty clinical and oral healthy ambulatory pre- and postmenopausal women from two southern Argentine cities: Comodoro Rivadavia (latitude 45º S) and Ushuaia (latitude 54º S) were included in the study. CTX levels were evaluated in serum, urine, and saliva, and b-ALP levels were measured in serum and saliva. In both groups of women, salivary CTX showed a maximum percentage of change early in the morning (80%) and a minimum in the late afternoon (45%), similarly to the pattern observed in urinary samples. No daily rhythm was observed in serum or salivary b-ALP. 25-Hydroxyvitamin D levels decreased in winter vs. summer (p < 0.01) without differences between the two studied groups. Conversely, parathormone reached higher levels in winter (p < 0.05) which induced a slight non-significant increment in salivary CTX and b-ALP levels. The results showed that, as in serum and urinary samples, salivary CTX exhibits daily and a slight seasonal rhythmicity. Whole non-stimulated saliva is a useful tool to detect several oral and systemic diseases because it has important advantages compared to serum and urinary samples. Then, it may also be a promising sample to test changes in bone metabolism contributing to diagnose and to monitor the therapy of several metabolic bone diseases.

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Activity or mass concentration of bone-specific alkaline phosphatase as a marker of bone formation

Cited by 25 publications

References 20 publications

5-Azacytidine facilitates osteogenic gene expression and differentiation of mesenchymal stem cells by alteration in DNA methylation

5-Azacytidine facilitates osteogenic gene expression and differentiation of mesenchymal stem cells by alteration in DNA methylation

The effects of combined training on bone metabolic markers in postmenopausal women

Salivary bone turnover markers in healthy pre- and postmenopausal women: daily and seasonal rhythm

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