Activity of Turbinaria Ornata (Turner) J. Agade Against Blue Tongue Virus (Btv)

Abstract

ABSTRACT:-A sensitive and accurate method was developed to test the efficacy of the aqueous extract of marine seaweed, Turbinaria ornata (Turner) J. Agardh against blue tongue virus using the 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) method. The abundance of this algae on the shores during the month of November and no reports so far ,having antiviral activity in addition to this other solvent extracts were toxic to the cell line hence water was selected as the solvent for extraction. On the other hand dextran sulphate was used a positive control and the bioactive compounds efficacy was compared with it. The optical density of formazan was used to determine cell viability. The IC 50 values of dextran sulphate and the extracts were found to be nearly similar to those obtained by the plaque reduction method.key words:-mtt, seaweed, bioactive, aqueous extract, formazan,IC50value ,dextran sulphate.

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“…Antiviral activity was evaluated using MTT assay according to (Sethi, 2016; Andrighetti-Fröhner et al, 2003) 35,36…”

“…Optical density should be directly correlated with cell quantity. The maximum non toxic concentration (MNTC) of each extract was determined and was used for further biological studies ( Table S2 and Figure S2 ) 35,36…”

“…The optical density is determined at 560 nm and the background is subtracted at 620 nm. Optical density should be directly correlated with cell quantity 35,36…”

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“…The virus infection of Vero cells was assayed by quantal assay to have 50 % tissue culture infectious dose end‐point (TCID50 %) and plaque formation unit (PFU) . The antiviral activity of the crude extract and pure compound was tested against Hepatitis A and Herpes simplex virus using MTT assay in VERO cell in the presence of acyclovir (=9‐(2‐hydroxyetho‐xymethyl)guanosine, Sigma) as the positive control for HSV 1 . The antiviral activity was evaluated at the maximum non‐toxic concentration (MNTC) of the tested materials then diluted at different concentrations against TCID50/mL of virus using MTT assay.…”

“…[32] The antiviral activity of the crude extract and pure compound was tested against Hepatitis A and Herpes simplex virus using MTT assay in VERO cell in the presence of acyclovir (= 9-(2-hydroxyetho-xymethyl)guanosine, Sigma) as the positive control for HSV 1. [33] The antiviral activity was evaluated at the maximum non-toxic concentration (MNTC) of the tested materials then diluted at different concentrations against TCID50/mL of virus using MTT assay. The inhibition percentage was calculated for each concentration using the following formula: (Absorbance of the treatedÀ absorbance of the control virus)/(Absorbance of the cellular controlÀ absorbance of the control virus) × 100.…”