Production of herbarin and dehydroherbarin by endophytic Chaetosphaeronema sp. (KY321184) isolated from Nepeta septemcrenata and evaluation of their bioactivities

Osman, Mohamed; El‐Beih, Ahmed A.; Khatab, O.-K.H.; Moghannem, Saad A.; Abdullah, Nashwa Hamed

doi:10.1016/j.sajb.2018.05.018

Cited by 7 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous reports, some naphthoquinone derivatives were reported as cytotoxic against various cancer cell lines [ 35 ], and specifically in breast cancer [ 36 ], but to our knowledge, there are no reports describing the effect of naphthoquinone derivatives in cancer spheroid models. Furthermore, the use of high-content cellular imaging provided additional valuable information.…”

Section: Discussionmentioning

confidence: 99%

Naphthoquinone Derivatives from Angustimassarina populi CF-097565 Display Anti-Tumour Activity in 3D Cultures of Breast Cancer Cells

Mackenzie,

Reyes,

Martínez

et al. 2024

Molecules

View full text Add to dashboard Cite

Cancer is one of the leading causes of death worldwide, with breast cancer being the second cause of cancer-related mortality among women. Natural Products (NPs) are one of the main sources for drug discovery. During a screening campaign focused on the identification of extracts from Fundación MEDINA’s library inhibiting the proliferation of cancer cell lines, a significant bioactivity was observed in extracts from cultures of the fungus Angustimassarina populi CF-097565. Bioassay-guided fractionation of this extract led to the identification and isolation of herbarin (1), 1-hydroxydehydroherbarin (4) plus other three naphthoquinone derivatives of which 3 and 5 are new natural products and 2 is herein described from a natural source for the first time. Four of these compounds (1, 3, 4 and 5) confirmed a specific cytotoxic effect against the human breast cancer cell line MCF-7. To evaluate the therapeutic potential of the compounds isolated, their efficacy was validated in 3D cultures, a cancer model of higher functionality. Additionally, an in-depth study was carried out to test the effect of the compounds in terms of cell mortality, sphere disaggregation, shrinkage, and morphology. The cell profile of the compounds was also compared to that of known cytotoxic compounds with the aim to distinguish the drug mode of action (MoA). The profiles of 1, 3 and 4 showed more biosimilarity between them, different to 5, and even more different to other known cytotoxic agents, suggesting an alternative MoA responsible for their cytotoxicity in 3D cultures.

show abstract

Section: Discussionmentioning

confidence: 99%

Naphthoquinone Derivatives from Angustimassarina populi CF-097565 Display Anti-Tumour Activity in 3D Cultures of Breast Cancer Cells

Mackenzie,

Reyes,

Martínez

et al. 2024

Molecules

View full text Add to dashboard Cite

show abstract

“…Nowadays, endophytic fungi have great interest as they represent several bioactive compounds with promising biological activities [ 70 ]. The endophytic colonization of O. ficus-indica in our study in Egypt differed from the results found in other studies in Brazil, as most of the fungal genera obtained as endophytes of O. ficus-indica in Brazil were described as Cladosporium cladosporioides (20.43%) and C. sphaerospermum (15.99%) [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Exploring the potential of Rhizopus oryzae AUMC14899 as a novel endophytic fungus for the production of l-tyrosine and its biomedical applications

2023

View full text Add to dashboard Cite

Background A significant threat to the public's health is the rise in antimicrobial resistance among numerous nosocomial bacterial infections. This may be a detriment to present initiatives to enhance the health of immune-compromised patients. Consequently, attention has been devoted to exploring new bioactive compounds in the field of drug discovery from endophytes. Therefore, this study is the first on the production of l-tyrosine (LT) as a promising bio-therapeutic agent from endophytic fungi. Results A new endophytic fungal isolate has been identified for the first time as Rhizopus oryzae AUMC14899 from Opuntia ficus-indica (L.) and submitted to GenBank under the accession number MZ025968. Separation of amino acids in the crude extract of this fungal isolate was carried out, giving a higher content of LT, which is then characterized and purified. LT exhibited strong antibacterial and anti-biofilm activities against multidrug-resistant Gram-negative and Gram-positive bacteria. The recorded minimum inhibitory concentration (MIC) values ranged from 6 to 20 µg/ml. In addition, LT caused a strong reduction in biofilm formation and disrupted the preformed biofilm. Moreover, results indicated that LT supported cell viability, evidencing hemocompatibility and no cytotoxicity. Conclusion Our findings suggest that LT has potential as a therapeutic agent due to its potential antibacterial, anti-biofilm, hemocompatibility, and lack of cytotoxic activities, which may also increase the range of therapy options for skin burn infections, leading to the development of a novel fungal-based drug.

show abstract

“…[84][85] Naturally occurring pyranonapthoquinone's derivatives possessed biological activities were obtained from various living organisms such as including fungi, bacteria and higher plants. Many compounds of pyranonapthoquinones like eleutherin (11), psychorubicin (12) and pentalongin (13) have been using globally for the treatment of antibacterial, antiviral, anti-parasitic and anticancer [86] αlapachone ( 14) and β-lapachones (15) are natural compounds, they were extracted from the heartwood of the Bignoniaceae trees [87] αand β-lapachone used for vast treatment, including anticancer, [88] antibacterial and antiinflammatory [89] activities, β-lapachone were used for the treatment of NADH quinone oxidoreductase tumours. [90] The compounds exist in level II clinical trials to cure a pancreatic tumour.…”

Section: Origin-based Pyran Derivativesmentioning

confidence: 99%

Insights into the Origin and Therapeutic Implications of Benzopyran and Its Derivatives

Tiwari

Singh

2023

ChemistrySelect

View full text Add to dashboard Cite

Benzopyran derivatives are essential for life and are found abundantly in nature. Benzopyran compounds and their derivatives play an essential role in medicinal chemistry. The alkaloids, flavonoids, tocopherols, and anthocyanins natural products comprise benzopyran's basic structure. Various benzopyran derivatives have been used for therapeutic effects. Benzopyran derivatives can be proficient in various pharmaceutical applications for cellular markers. The basic structure of benzopyran is an efficient class of synthetic products and an emerging attention area of research. Therefore, impactful information on benzopyran and its derivatives has been compiled in the last several years. Recently, the development of novel benzopyran and its applications are contributing to medicines and pharmacy.

show abstract

Production of herbarin and dehydroherbarin by endophytic Chaetosphaeronema sp. (KY321184) isolated from Nepeta septemcrenata and evaluation of their bioactivities

Cited by 7 publications

References 26 publications

Naphthoquinone Derivatives from Angustimassarina populi CF-097565 Display Anti-Tumour Activity in 3D Cultures of Breast Cancer Cells

Naphthoquinone Derivatives from Angustimassarina populi CF-097565 Display Anti-Tumour Activity in 3D Cultures of Breast Cancer Cells

Exploring the potential of Rhizopus oryzae AUMC14899 as a novel endophytic fungus for the production of l-tyrosine and its biomedical applications

Insights into the Origin and Therapeutic Implications of Benzopyran and Its Derivatives

Contact Info

Product

Resources

About