Activity of physalins purified from Physalis angulata in in vitro and in vivo models of cutaneous leishmaniasis

Guimarães, Elisalva Teixeira; Lima, Milena S.; Santos, Luciane Amorim; Ribeiro, Ícaro José Santos; Tomassini, Therezinha Coelho Barbosa; Santos, Ricardo Ribeiro dos; Santos, Washington Luis Conrado dos; Soares, Milena Botelho Pereira

doi:10.1093/jac/dkp170

Cited by 72 publications

(44 citation statements)

References 10 publications

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“…Physalins B and F were able to reduce the percentage of Leishmania-infected macrophages and the intracellular parasite number. The topical treatment with physalin F reduced the lesion size, the parasite load and histopathological alterations in BALB/c mice infected with L. amazonensis [9]. In this study, perhaps the content of the active substances in the extracts is low.…”

Section: Discussionmentioning

confidence: 55%

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Anti-promastigote Activity of the Amazon Plants

Silva-Silva¹,

Monteiro²,

Brígido³

et al. 2017

JPP

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This study evaluated in vitro activity of ethanol extract, fractions, and isolated substance from Amazon species against promastigotes of L. amazonensis. The ethanol extracts were concentrated and fractionation. The anti-promastigote activity was evaluated through the cell viability assessment method (MTT). The ethanol extract, fractions, and isolated substance from Himatanthus articulatus and Parahancornia fasciculata were inactive in promastigote of L. amazonensis, as the ethanol extract of Physalis angulata. The hexane fractions from different parts of Montrichardia linifera showed anti-promastigote activity probably due to the presence of steroids and terpenes. All species in studies were inactive, except of M. linifera. The few polar constituents can be responsible for the activity. Therefore, the isolation and purification of the active on L. amazonensis promastigotes are urgently required.

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Section: Discussionmentioning

confidence: 55%

“…angulata showed activity against L. major and L. amazonensis [9]. This study evaluated the inhibitory activity of …”

Section: Leishmaniasis Is Caused By the Protozoanmentioning

confidence: 99%

Anti-promastigote Activity of the Amazon Plants

Silva-Silva¹,

Monteiro²,

Brígido³

et al. 2017

JPP

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“…species, primarily to identify and assess substances with possible medicinal value (Guimarães et al 2009, Sá et al 2011. However, just a few researches have investigated its cultivation or management, including Souza & Amorim (2009), who assessed the growth and development of the species under water deficit, and Cruz et al (2015), who evaluated the influence of phosphate fertilizer on its cultivation.…”

Section: Abstract Resumomentioning

confidence: 99%

Hydroponic cultivation of Physalis angulata L.: growth and production under nitrogen doses1

Leite

Tanan

Nascimento

et al. 2017

Pesqui. Agropecu. Trop.

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The Physalis angulata L. species has attracted interest due to the production of compounds with pharmacological activity and its potential for fruiticulture. Given that it is a fast-growing and highly productive species, determining the most adequate nitrogen (N) doses could contribute to higher crop yields. This study aimed at assessing the influence of N concentrations, in a hydroponic system, on the growth and production of P. angulata, as well as determining the critical N level in leaves. The experiment was conducted in individual pots with nutrient solutions, applying a completely randomized design and twelve replications, using five N doses (0 mg L-1, 56 mg L-1, 112 mg L-1, 168 mg L-1 and 224 mg L-1). Growth and production indices, amount of total N on leaves and stems and critical N levels were assessed. The increase of N doses in the nutrient solution influenced plant growth and fruit production, as well as the accumulation of total N in the leaves and stems. Based on the maximum economic yield, a dose of 162 mg L-1 of N is recommended for hydroponics, which provided a fruit yield of 7.27 g m-2 and critical total N level in leaves of 51.98 g kg-1.

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“…The compounds responsible for the activity are not yet known. Some limonoids were nevertheless shown to be active against leishmaniasis (Guimaraes et al, 2009). Other known compounds from P. angulata include whithanolides, withangulatins (steroidal lactones) and physalins (unusual steroids) (Damu et al, 2007).…”

Section: Melia Azedarach L (Meliaceae)mentioning

confidence: 99%

In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

Lusakibanza

Mesia

Tona

et al. 2010

Journal of Ethnopharmacology

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Aim of the studyThe in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ethnobotanical survey conducted in D.R. Congo and focusing on plants used traditionally to treat malaria. The in vivo antimalarial activity of aqueous and methanolic extracts active in vitro was also determined in mice infected by Plasmodium berghei berghei. Materials and methodsThe growth inhibition of Plasmodium falciparum strains was evaluated using the measurement of lactate dehydrogenase activity. The extracts (aqueous, CH 3 OH, EtOH and CH 2 Cl 2 ) were prepared by maceration and tested in vitro against the 3D7 (chloroquine sensitive) and W2 (chloroquine resistant) strains of Plasmodium falciparum and against the human normal fetal lung fibroblasts WI-38 to determine the selectivity index. Some extracts were also used at the dose of 300mg/kg to evaluate their activity in mice infected since 4 days by Plasmodium berghei. ResultsTwo plants presented a very high activity (IC 50 < 3µg/ml). These plants were Strychnos icaja roots bark (MeOH and CH 2 Cl 2 ) and Physalis angulata leaves (MeOH and CH 2 Cl 2 ). One plant (Anisopappus chinensis whole plant, MeOH and CH 2 Cl 2 ) presented a high activity (IC50 < 15µg/ml).The extracts of Anisopappus chinensis and Physalis angulata showed also a good inhibition of parasitemia in vivo. Flavonoids, phenolic acids and terpenes were identified in these plants by a general phytochemical screening method. ConclusionThree plants showed a very interesting antiplasmodial activity (Anisopappus chinensis, Physalis angulata, Strychnos icaja) and one of them showed a good selectivity index ( >10, Anisopappus chinensis). Anisopappus chinensis and Physalis angulata were also active in vivo.

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Activity of physalins purified from Physalis angulata in in vitro and in vivo models of cutaneous leishmaniasis

Abstract: Our results demonstrate the potent antileishmanial activity of physalins, especially physalin F, and suggest these molecules as the basis for the development of new therapeutic options for cutaneous leishmaniasis.

Cited by 72 publications

References 10 publications

Anti-promastigote Activity of the Amazon Plants

Anti-promastigote Activity of the Amazon Plants

Hydroponic cultivation of Physalis angulata L.: growth and production under nitrogen doses1

In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine

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