Activity of IgG and IgM ABO Antibodies against Some Weak A (A(3), A(x), and A(end)) and Weak B (B(3), B(x)) Red Cells

Abstract

The IgG and IgM anti-A and anti-B activities from several immune and non-immune O, A and B sera were tested against a panel of weak A(A(3), A(x), and A(end)) and weak B (B(3) and B(x)) red cells. In all cases it is the IgM which agglutinated optimally A(x) (or Bx) cells, while IgG and IgM anti-A (or anti-B) reacted similarly with A(3) and A(end) (or B(3)) cells. The agglutinating activity of all these ABO antibodies was found straightly related to their association constant for the A (or the B) receptor.

Blood group discrepancies at a tertiary care centre – analysis and resolution

“…Polyclonal antisera of human origin from group B, group A, and group O individuals were used for adsorption to determine these subgroups [4]. The technique was performed as described in AABB technical manual [5].…”