Review: recent progress in the molecular genetic study of the histo-blood group ABO system

Yamamoto, Fumiichiro

doi:10.21307/immunohematology-2019-810

Cited by 12 publications

(1 citation statement)

References 46 publications

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“…The reason for this discrepancy is unclear, but might depend on the monoclonal antibodies used. Alternatively, this is due to changes in the ABO antigens, caused by differentiation and/or maturation, although altered glycosylation is usually associated with malignant transformation [17]. Extensive expression of blood group A was seen in the parathyroid cells, in analogue with previous studies [18,19].…”

Section: Discussionsupporting

confidence: 82%

Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands

Säljö

Thornell

Jin

et al. 2021

IJMS

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As part of a systematic investigation of the glycosphingolipids in human tissues, acid and non-acid glycosphingolipids from human thyroid and parathyroid glands were isolated and characterized with mass spectrometry and binding of carbohydrate-recognizing ligands, with a focus on complex compounds. The glycosphingolipid patterns of the human parathyroid and thyroid glands were very similar. The major acid glycosphingolipids were sulfatide and the gangliosides GM3, GD3, GD1a, GD1b, GT1b and Neu5Ac-neolactotetraosylceramide, and the major non-acid glycosphingolipids were globotriaosylceramide and globoside. We also found neolactotetra- and neolactohexaosylceramide, the x2 glycosphingolipid, and complex glycosphingolipids with terminal blood group O and A determinants in both tissues. A glycosphingolipid with blood group Leb determinant was identified in the thyroid gland, and the parathyroid sample had a glycosphingolipid with terminal blood group B determinant. Immunohistochemistry demonstrated the expression of blood group A antigens in both the thyroid and parathyroid glands. A weak cytoplasmatic expression of the GD1a ganglioside was present in the thyroid, while the parathyroid gland had a strong GD1a expression on the cell surface. Thus, the glycosylation of human thyroid and parathyroid glands is more complex than previously appreciated. Our findings provide a platform for further studies of alterations of cell surface glycosphingolipids in thyroid and parathyroid cancers.

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Section: Discussionsupporting

confidence: 82%

Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands

Säljö

Thornell

Jin

et al. 2021

IJMS

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A rapid and reliable PCR method for genotyping the ABO blood group. II: A2 and O2 alleles

O’Keefe

Dobrovic

1996

Hum. Mutat.

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A novel technique for detecting single nucleotide polymorphisms by analyzing consumed allele-specific primers

et al. 2001

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We present a simple and rapid polymerase chain reaction (PCR)-based technique, termed consumed allele-specific primer analysis (CASPA), as a new strategy for single nucleotide polymorphism (SNP) analysis. The method involves the use of labeled allele-specific primers, differing in length, with several noncomplementary nucleotides added in the 5'-terminal region. After PCR amplification, the amounts of the remaining primers not incorporated into the PCR products are determined. Thus, nucleotide substitutions are identified by measuring the consumption of primers. In this study, the CASPA method was successfully applied to ABO genotyping. In the present method, the allele-specific primer only anneals with the target polymorphic site on the DNA, so it is not necessary to analyze the PCR products. Therefore, this method is only little affected by modification of the PCR products. The CASPA method is expected to be a useful tool for typing of SNPs.

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Review: recent progress in the molecular genetic study of the histo-blood group ABO system

Cited by 12 publications

References 46 publications

Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands

Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands

A rapid and reliable PCR method for genotyping the ABO blood group. II: A2 and O2 alleles

A novel technique for detecting single nucleotide polymorphisms by analyzing consumed allele-specific primers

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