Activity and Thermal Stability Improvements of Glucose Oxidase upon Adsorption on Core−Shell PMMA−BSA Nanoparticles

Abstract

The interaction and adsorption of enzyme, glucose oxidase (GOx), on poly(methyl methacrylate)-bovine serum albumin (PMMA-BSA) particles were studied by using a quartz crystal microbalance with dissipation (QCM-D) and laser light scattering (LLS). The enzyme was irreversibly immobilized on the PMMA-BSA particle surface. The amount of enzyme immobilized on PMMA-BSA particles and the enzymatic activity were determined by UV/vis measurements. The influences of pH and ionic strength on the adsorption indicate that the electrostatic interaction plays a major role on the immobilization. The adsorbed GOx can retain at least 80% of the free enzyme activity. Thermal stability studies reveal that the adsorbed GOx only losses 28% of its activity in comparison with a 64% activity loss of free GOx when it is incubated at 50 degrees C for 35 h.

A Comprehensive Review of Glucose Biosensors Based on Nanostructured Metal-Oxides

“…Because the IEP of ZrO 2 is of 4.15 [196], it is suitable for the adsorption of high IEP proteins. Therefore, any other nanomaterial, which can drop down the IEP of ZrO 2 when it is mixed with ZrO 2 is needed for the application of ZrO 2 -based electrode to glucose sensing since the IEP of glucose is of 4.2 [197]. The first amperometric glucose sensor based on sol-gel derived ZrO 2 was reported by Liu et al [198].…”

Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis

“…The higher stability may be attributed to the multipoint covalent binding of the protease to the support which restricts the flexibility and conformational mobility of the enzyme, thus preventing unfolding or denaturation [27]. Immobilization onto solid supports has often led to increased thermal stability of the enzymes [32,33]. Fungal protease encapsulated within silicated nanospheres exhibited enhanced thermal stability and retained 100 % stability at any incubation temperature up to 70°C [33].…”

Silica-encapsulated magnetic nanoparticles: Enzyme immobilization and cytotoxic study

“…There are some literature reports including immobilization of GOD onto magnetic nanostructures [7,9,10,16,26,27,34,36,39,40]. A comparison between the expected advantages of immobilized enzymes in terms of reusability and storage stability of the prepared silica encapsulated GOD-MNPs with those of the previously reported ones have been summarized in Table 2.…”