Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis

Sinha, Rajeshwari; Khare, Sunil Kumar

doi:10.1007/s00449-014-1314-2

Cited by 24 publications

(8 citation statements)

References 43 publications

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“…5C and Fig. 5D, there were broad and strong peaks at 3,325 cm -1 and 3,344 cm -1 , which indicated the vibration modes of the O-H and -NH groups from enzymes that interact with nanoparticles, which has been suggested previously [19].…”

Section: Characterization Of the Functionalized Nanoparticles Used Fosupporting

confidence: 74%

“…The degree of hydrolysis was determined by quantifying the soluble protein content after precipitation with TCA [19,26]. 1ml of protein hydrolysate was mixed with 1 ml of 10%…”

Section: Degree Of Hydrolysismentioning

confidence: 99%

“…however there are only a few studies that have exploited MNPs for crude protease immobilisation and further use in protein hydrolysis and synthesis [19].…”

Section: Introductionmentioning

confidence: 99%

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The immobilisation of proteases produced by SSF onto functionalized magnetic nanoparticles: Application in the hydrolysis of different protein sources

Yazid

Barrena

Sánchez

2016

Journal of Molecular Catalysis B: Enzymatic

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Alkaline proteases produced from protein-rich waste (hair waste and soya residues) by solid state fermentation (SSF) were immobilised onto functionalized magnetic iron oxide nanoparticles (MNPs) using glutaraldehyde as a crosslinking agent. The covalent binding method had a better immobilisation yield compared to simple adsorption, retaining 93%-96% (459±106 U/mg nanoparticles, 319±34 U/mg nanoparticles) of hair waste and soya residues proteases, respectively after crosslinking with 5% glutaraldehyde for 6 h. However, the adsorption immobilisation yield was 47%-54% after 8 h for both proteases. MNPs and immobilised proteases were characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and electron diffraction. Our results indicated successful crosslinking between the proteases and amino-functionalized MNPs. The operational stability (pH and temperature) and storage stability of free and immobilised enzyme were also analysed. Despite the fact that the optimum pH of free and immobilised proteases was identical in the alkaline region, the immobilised proteases reached their optimum condition at higher temperatures (40ºC-60ºC 40 ºC-60 ºC). After 2 months of storage at 4ºC 4 ºC, the immobilised proteases showed good stability, retaining more than 85% of their initial activity. The high magnetic response of MNPs render an ease of separation and reusability, which contributes to the residual activity of both immobilised proteases on MNPs remained retaining more than 60% of their initial values after seven hydrolytic cycles. These results resulted showed the enhancement of the stability of the crosslinking interactions between the proteases and nanoparticles. The immobilised proteases were capable of hydrolysing select selected proteins (casein, oat bran protein isolate, and egg white albumin). However, differences in the degree of hydrolysis were observed, depending on the combination of the protease and type of substrate used.

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Section: Characterization Of the Functionalized Nanoparticles Used Fosupporting

confidence: 74%

“…The degree of hydrolysis was determined by quantifying the soluble protein content after precipitation with TCA [19,26]. 1ml of protein hydrolysate was mixed with 1 ml of 10%…”

Section: Degree Of Hydrolysismentioning

confidence: 99%

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The immobilisation of proteases produced by SSF onto functionalized magnetic nanoparticles: Application in the hydrolysis of different protein sources

Yazid

Barrena

Sánchez

2016

Journal of Molecular Catalysis B: Enzymatic

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“…These inherent capabilities make nanomaterials valuable for a wide variety of biotechnological applications including biosensing, drug delivery, bioremediation, biofuel production, antibacterial activity and disease diagnostics [14 -24]. Nanomaterials have the ability to enhance the performance and activity of the immobilized enzyme [25,26], as they influence the factors that determine the efficiency of biocatalyst, such as surface area to volume ratio, mass transfer resistance and effective enzyme loading [3, 27 -35].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Cholesterol Oxidase: An Overview

Ghosh¹,

Ahmad²,

Khare³

2018

TOBIOTJ

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Background: Cholesterol oxidases are bacterial oxidases widely used commercially for their application in the detection of cholesterol in blood serum, clinical or food samples. Additionally, these enzymes find potential applications as an insecticide, synthesis of anti-fungal antibiotics and a biocatalyst to transform a number of sterol and non-sterol compounds. However, the soluble form of cholesterol oxidases are found to be less stable when applied at higher temperatures, broader pH range, and incur higher costs. These disadvantages can be overcome by immobilization on carrier matrices. Methods: This review focuses on the immobilization of cholesterol oxidases on various macro/micro matrices as well as nanoparticles and their potential applications. Selection of appropriate support matrix in enzyme immobilization is of extreme importance. Recently, nanomaterials have been used as a matrix for immobilization of enzyme due to their large surface area and small size. The bio-compatible length scales and surface chemistry of nanoparticles provide reusability, stability and enhanced performance characteristics for the enzyme-nanoconjugates. Conclusion: In this review, immobilization of cholesterol oxidase on nanomaterials and other matrices are discussed. Immobilization on nanomatrices has been observed to increase the stability and activity of enzymes. This enhances the applicability of cholesterol oxidases for various industrial and clinical applications such as in biosensors.

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“…The immobilization of bioactive macromolecules such as proteins (e.g., enzymes and antibodies), polysaccharides, and nucleic acids onto inorganic support is almost compulsory for their applications in various industrial and biomedical fields such as antibody purification and enzyme catalysis [1,2]. An appropriately designed immobilization can not only keep a good balance between rigidity and flexibility of target proteins and enzyme molecules, but also improve the biological compatibility of the inorganic supports and increase the interactions between the supports and the proteins.…”

Section: Introductionmentioning

confidence: 99%

Optimizing immobilization of avidin on surface-modified magnetic nanoparticles: characterization and application of protein-immobilized nanoparticles

Yang

Sun

et al. 2015

Bioprocess Biosyst Eng

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A simple optimization method of immobilization of avidin on magnetic nanoparticles (MNPs)' surface was proposed in this study. The avidin-immobilized MNPs were then developed and used to immobilize a model enzyme [Horseradish peroxidase (HRP)]. The loading capacity (LC) and activity of avidin-immobilized MNPs were optimized through selecting the most appropriate nanoparticle's size and shape, glutaraldehyde concentration, cross-linking reaction time, ultrasonic processing time, and initial concentration of avidin. The LC under optimized conditions was 63.37 ± 1.29 mg avidin/g MNPs, and the immobilized protein was still able to maintain its high biological activity of 10.86 ± 0.13 U/mg (biotin-binding activity of nature avidin was 14.1 U/mg) and better thermal stability compared to free avidin. A highly reusable, stable, and easily recovered immobilized HRP was obtained using MNPs as carriers. The immobilized HRP was reused repeatedly more than 9 times and retained more than 65 % of its original activity.

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Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis

Cited by 24 publications

References 43 publications

The immobilisation of proteases produced by SSF onto functionalized magnetic nanoparticles: Application in the hydrolysis of different protein sources

The immobilisation of proteases produced by SSF onto functionalized magnetic nanoparticles: Application in the hydrolysis of different protein sources

Immobilization of Cholesterol Oxidase: An Overview

Optimizing immobilization of avidin on surface-modified magnetic nanoparticles: characterization and application of protein-immobilized nanoparticles

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