Optimizing immobilization of avidin on surface-modified magnetic nanoparticles: characterization and application of protein-immobilized nanoparticles

Yang, Tao; Sun, Shuguo; Ma, Meihu; Lin, Qinlu; Zhang, Lin; Li, Yan; Luo, Feijun

doi:10.1007/s00449-015-1443-2

Cited by 4 publications

(2 citation statements)

References 49 publications

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“…IOP labeling has been combined with detection of lipophilic fluorescent probes [99,100] and genetically encoded fluorescent proteins [45,101]. Other potential combinations include the use of Q-dots and fluorescently conjugated dextrans [102,103].…”

Section: Combination Of Iops With Other Tracer Modalitiesmentioning

confidence: 99%

In vivo Cell Tracking Using Non-invasive Imaging of Iron Oxide-Based Particles with Particular Relevance for Stem Cell-Based Treatments of Neurological and Cardiac Disease

et al. 2019

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Stem cell-based therapeutics is a rapidly developing field associated with a number of clinical challenges. One such challenge lies in the implementation of methods to track stem cells and stem cell-derived cells in experimental animal models and in the living patient. Here, we provide an overview of cell tracking in the context of cardiac and neurological disease, focusing on the use of iron oxide-based particles (IOPs) visualized in vivo using magnetic resonance imaging (MRI). We discuss the types of IOPs available for such tracking, their advantages and limitations, approaches for labeling cells with IOPs, biological interactions and effects of IOPs at the molecular and cellular levels, and MRI-based and associated approaches for in vivo and histological visualization. We conclude with reviews of the literature on IOP-based cell tracking in cardiac and neurological disease, covering both preclinical and clinical studies.

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Section: Combination Of Iops With Other Tracer Modalitiesmentioning

confidence: 99%

In vivo Cell Tracking Using Non-invasive Imaging of Iron Oxide-Based Particles with Particular Relevance for Stem Cell-Based Treatments of Neurological and Cardiac Disease

et al. 2019

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“…(3) The calculated peak was compared against the experimentally observed IR spectral peak. (4) The bond and angle constant values were iteratively updated, and the next cycle of simulation and calculation was done until the Fe–O IR peak fit with the experimental characteristic peak of Fe 3 O 4 near 570 cm –1 . The simulated final IR spectrum is shown in Figure a.…”

Section: Methodsmentioning

confidence: 99%

Solvent Effects in Ligand Stripping Behavior of Colloidal Nanoparticles

et al. 2023

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Inorganic colloidal nanoparticle (NP) properties can be tuned by stripping stabilizing ligands using a poor solvent. However, the mechanism behind ligand stripping is poorly understood, in part because in situ measurements of ligand stripping are challenging at the nanoscale. Here, we investigate ethanol solvent-mediated oleylamine ligand stripping from magnetite (Fe3O4) NPs in different compositions of ethanol/hexane mixtures using atomistic molecular dynamics (MD) simulations and thermogravimetric analysis (TGA). Our study elucidates a complex interplay of ethanol interactions with system components and indicates the existence of a threshold concentration of ∼34 vol % ethanol, above which ligand stripping saturates. Moreover, hydrogen bonding between ethanol and stripped ligands inhibits subsequent readsorption of the ligands on the NP surface. A proposed modification of the Langmuir isotherm explains the role of the enthalpy of mixing of the ligands and solvents on the ligand stripping mechanism. A good agreement between the MD predictions and TGA measurements of ligand stripping from Fe3O4 NPs validates the simulation observations. Our findings demonstrate that the ligand coverage of NPs can be controlled by using a poor solvent below the threshold concentration and highlight the importance of ligand–solvent interactions that modulate the properties of colloidal NPs. The study also provides an approach for a detailed in silico study of ligand stripping and exchange from colloidal NPs that are crucial for applications of NPs spanning self-assembly, optoelectronics, nanomedicine, and catalysis.

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Nanobody immobilization on magnetic nanoparticles via monomeric streptavidin-biotin specific interaction for aflatoxin adsorption

Wang,

Tarabarov,

Rao

et al. 2024

Adv Compos Hybrid Mater

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Optimizing immobilization of avidin on surface-modified magnetic nanoparticles: characterization and application of protein-immobilized nanoparticles

Cited by 4 publications

References 49 publications

In vivo Cell Tracking Using Non-invasive Imaging of Iron Oxide-Based Particles with Particular Relevance for Stem Cell-Based Treatments of Neurological and Cardiac Disease

In vivo Cell Tracking Using Non-invasive Imaging of Iron Oxide-Based Particles with Particular Relevance for Stem Cell-Based Treatments of Neurological and Cardiac Disease

Solvent Effects in Ligand Stripping Behavior of Colloidal Nanoparticles

Nanobody immobilization on magnetic nanoparticles via monomeric streptavidin-biotin specific interaction for aflatoxin adsorption

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