A Comprehensive Review of Glucose Biosensors Based on Nanostructured Metal-Oxides

Rahman, Md. Mahbubur; Ahammad, A. J. Saleh; Jin, Joon-Hyung; Ahn, Sang Jung; Lee, Jae‐Joon

doi:10.3390/s100504855

Cited by 685 publications

(376 citation statements)

References 227 publications

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“…GOx presents two possible electron transfer mechanisms, one through the breakdown of the by-product hydrogen peroxide and the other through the direct electron transfer from FADH2. The mechanism at play varies depending upon the distance between the catalytic site on the surface of the electrode and the mediator used in the buffer [22]. Because of these well-known properties, GOx is an ideal model enzyme for studying the electron transfer pathway for new material interfaces such as ZnO/PC.…”

Section: Introductionmentioning

confidence: 99%

Mediator-free interaction of glucose oxidase, as model enzyme for immobilization, with Al-doped and undoped ZnO thin films laser-deposited on polycarbonate supports

V.T.K.P.

Inguva

Krishnamurthy

et al. 2017

Enzyme and Microbial Technology

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Research Highlights: Electrochemical interaction of ZnO and AZO thin films with the enzyme glucose oxidase was studied.  Nature of interaction of GOx with AZO was explored using XPS.  Mechanism of the electron transfer between GOx and AZO/ZnO was studied using EIS.  The results show that AZO is preferable to ZnO for GOx immobilization and glucose sensor development. Abstract:Al doped and undoped ZnO thin films were deposited by pulsed-laser deposition on polycarbonate sheets. The films were characterized by optical transmission, Hall effect measurement, XRD and SEM. Optical transmission and surface reflectometry studies showed good transparency with thicknesses ~100 nm and surface roughness of 10 nm. Hall effect measurements showed that the sheet carrier concentration was -1.44 x 10 15 cm -2 for AZO and -6 x 10 14 cm -2 for ZnO. The films were then modified by drop-casting glucose oxidase (GOx) without the use of any mediators. Higher protein concentration was observed on ZnO as compared to AZO with higher specific activity for ZnO (0.042 U mg -1 ) compared to AZO (0.032 U mg -1 ), and was in agreement with cyclic voltemmetry (CV). X-ray photoelectron spectroscopy (XPS) suggested that the protein was bound by dipole interactions between AZO lattice oxygen and the amino group of the enzyme. Chronoamperometry showed sensitivity of 5.5 µA mM -1 cm -2 towards glucose for GOx/AZO and 2.2 µA mM -1 cm -2 for GOx/ZnO. The limit of detection (LoD) was 167 µM of glucose for GOx/AZO, as compared to 360 µM for GOx/ZnO. The linearity was 0.28-28 mM for GOx/AZO whereas it was 0.6-28 mM for GOx/ZnO with a response time of 10s. Possibly due to higher enzyme loading, the decrease of impedance in presence of glucose was larger for GOx/ZnO as compared to GOx/AZO in electrochemical impedance spectroscopy (EIS). Analyses with clinical blood serum samples showed that the systems had good reproducibility and accuracy. The characteristics of novel ZnO and AZO thin films with GOx as a model enzyme, should prove useful for the future fabrication of inexpensive, highly sensitive, disposable electrochemical biosensors for high throughput diagnostics.

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Section: Introductionmentioning

confidence: 99%

Mediator-free interaction of glucose oxidase, as model enzyme for immobilization, with Al-doped and undoped ZnO thin films laser-deposited on polycarbonate supports

V.T.K.P.

Inguva

Krishnamurthy

et al. 2017

Enzyme and Microbial Technology

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“…Fig. 7 shows the cyclic voltammogram of redox current peaks for ampicillin nanoparticles at five times which illustrated the good stability of the cyclic voltammogram of the GCE by overlapping the lines [20]. …”

Section: Reliability and Stability Of Gce In Blood Mediummentioning

confidence: 99%

Effect of Micro- and Nanoparticles of Ampicillin Trihydrate on Blood Medium: A Voltammetric Study

Radhi¹,

Hamed²,

Ezzaldeen³

et al. 2017

Nano BioMed ENG

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For the first time, one of the antibiotic nanoparticles such as a classical form of ampicillin trihydrate compound was studied. The electrochemical behavior of ampicillin nanoparticles was investigated in blood medium using cyclic voltammetric technique by glassy carbon electrode. The results showed that the oxidation-reduction current peaks of ampicillin nanoparticles in blood medium were different from that of microparticles. The nanoparticles acted as anti-oxidative antibiotic by making the oxidation current peak at 1 V disappear, while the oxidation peak of microparticles still appeared in blood medium; hence, ampicillin at microform acted as oxidative reagent in blood medium. A good reliability and stability of glassy carbon electrode in blood medium was found with low values of RSD for oxidation-reduction current peaks at ±0.52% and ±0.038% respectively. Scanning electron microscopy for the characterization of ampicillin trihydrate nanoparticles was studied.

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“…Nanotechnology has opened up new opportunities to obtain more efficient electron mediators, such as metal nanoparticles or nanocarbons (i.e. carbon nanotubes or graphene) (Carralero et al, 2005;Rahman, Ahammad, Jin, Ahn, & Lee, 2010).…”

Section: Introductionmentioning

confidence: 99%

The advantages of disposable screen-printed biosensors in a bioelectronic tongue for the analysis of grapes

Medina-Plaza

García-Hernández

Saja

et al. 2015

LWT - Food Science and Technology

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a b s t r a c tDisposable screen-printed sensors have been modified with enzymes and used to form a bioelectronic tongue dedicated to the discrimination between different grape varieties. The multisensory system combined serigraphied electrodes modified with carbon, platinum, gold, graphene, Prussian blue and nickel oxide nanoparticles (M-SPE) covered with glucose oxidase (M-GOX-SPE) or tyrosinase (M-Tyr-SPE).The M-GOX-SPE and M-Tyr-SPE sensors produced a variety of responses due to the different behavior of the electron mediators of the six screen-printed materials used for the electro-catalysis of the glucose and phenols by means of glucose oxidase and tyrosinase.This variety of responses, together with the capability of the sensors to detect glucose or phenols, allowed the bioelectronic tongue developed here to discriminate between the juices obtained from different varieties of grape.Partial least-squares (PLS-1) multivariate calibration of electrochemical data has been successfully applied to the simultaneous determination of glucose and polyphenols in musts.The discrimination capability shown by this array of cheap and single-use sensors was similar to that found in other complex bioelectronic tongues. The lower price, ease of use and portability of the modified screen-printed electrode system makes the bioelectronic tongue developed here an alternative tool that can be used in situ in the vineyard block.

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A Comprehensive Review of Glucose Biosensors Based on Nanostructured Metal-Oxides

Cited by 685 publications

References 227 publications

Mediator-free interaction of glucose oxidase, as model enzyme for immobilization, with Al-doped and undoped ZnO thin films laser-deposited on polycarbonate supports

Mediator-free interaction of glucose oxidase, as model enzyme for immobilization, with Al-doped and undoped ZnO thin films laser-deposited on polycarbonate supports

Effect of Micro- and Nanoparticles of Ampicillin Trihydrate on Blood Medium: A Voltammetric Study

The advantages of disposable screen-printed biosensors in a bioelectronic tongue for the analysis of grapes

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