Activities of enzymes that hydrolyze adenine nucleotides in platelets from patients with rheumatoid arthritis

Activities of enzymes that hydrolyze adenine nucleotides in lymphocytes from patients with rheumatoid arthritis

“…Because most eukaryotic cells express purinergic system enzymes such as ecto‐nucleotide pyrophosphatase/phosphodiesterase (E‐NPP), E‐NTPDase, E‐5′‐nucleotidase and E‐ADA, a suitable explanation to these conflicting results would be an altered enzymatic kinetics in the different set of cells from the same group of patients. Corroborating with this hypothesis, the investigation of E‐ADA activity in rheumatoid synovial fibroblasts and platelets from patients with RA showed an increased enzymatic activity . We suggest that the decreased E‐ADA activity observed in this study would be a dynamic response of lymphocytes in an attempt to increase the adenosine concentrations.…”

Free and nanoencapsulated vitamin D3: effects on E‐NTPDase and E‐ADA activities in an animal model with induced arthritis

“…In addition, a lower E‐ADA activity in patients with RA has been reported in the literature . According to other studies previously published by our research group, the level of adenosine in the serum of RA patients decreased . Recently, a relationship between disease activity and serum E‐ADA levels has been reported, which, in turn, may predict actual disease activity, as well as the response of treatment in RA .…”

“…45 According to other studies previously published by our research group, the level of adenosine in the serum of RA patients decreased. 47 Recently, a relationship between disease activity and serum E-ADA levels has been reported, which, in turn, may predict actual disease activity, as well as the response of treatment in RA. 33 It is important to note that adenosine, an ADA substrate, exhibits potent anti-inflammatory and immunosuppressive actions by inhibiting the proliferation of T cells, the secretion of cytokines and the migration of leukocytes across endothelial barriers.…”

Purinergic system ecto-enzymes participate in the thromboregulation of patients with indeterminate form of Chagas disease

“…In patients with rheumatoid arthritis, an increase in the E-NTPDase, E-NPP, and E-5′-NT activities was found in platelets and discussed as a physiological response to an excessive platelet aggregation which occurs during the inflammatory process. However, an increased E-ADA activity was observed and associated to accelerated atherosclerosis found in these patients, due to decrease in adenosine production [21].…”