Purinergic system ecto-enzymes participate in the thromboregulation of patients with indeterminate form of Chagas disease

Souza, Viviane do Carmo Gonçalves; Schlemmer, Karine Bizzi; Noal, Cristiano Bicca; Jaques, Jeandre A.; Bagatini, Margarete Dulce; Pimentel, Victor Câmera; Carli, Luiz Felipe Dias; Leal, Cláudio A.M.; Fleck, Juliana; Moretto, Maria Beatriz; Schetinger, Maria Rosa Chitolina; Leal, Daniela Bitencourt Rosa

doi:10.1007/s11302-012-9322-7

Cited by 19 publications

(9 citation statements)

References 49 publications

(52 reference statements)

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“…In addition, serum activity was significantly higher in seropositive patients and there was a linear trend to increase with the clinical phase of the disease. These results are different from those reported by previous studies in patients with Chagas disease 6 , 28 , 29 . Although one could argue that the differences observed in the present study, with respect to the previous reports are due to the sample type and the absence of healthy controls to compare the values obtained in chagasic patients; it is more prudent to analyze the results in an appropriate pathogenic context.…”

Section: Discussioncontrasting

confidence: 99%

Adenosine Deaminase Activity and Serum C-Reactive Protein as Prognostic Markers of Chagas Disease Severity

Bravo-Tobar

Nello-Pérez

Fernández

et al. 2015

Rev. Inst. Med. trop. S. Paulo

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SUMMARYChagas disease is a public health problem worldwide. The availability of diagnostic tools to predict the development of chronic Chagas cardiomyopathy is crucial to reduce morbidity and mortality. Here we analyze the prognostic value of adenosine deaminase serum activity (ADA) and C-reactive protein serum levels (CRP) in chagasic individuals. One hundred and ten individuals, 28 healthy and 82 chagasic patients were divided according to disease severity in phase I (n = 35), II (n = 29), and III (n = 18). A complete medical history, 12-lead electrocardiogram, chest X-ray, and M-mode echocardiogram were performed on each individual. Diagnosis of Chagas disease was confirmed by ELISA and MABA using recombinant antigens; ADA was determined spectrophotometrically and CRP by ELISA. The results have shown that CRP and ADA increased linearly in relation to disease phase, CRP being significantly higher in phase III and ADA at all phases. Also, CRP and ADA were positively correlated with echocardiographic parameters of cardiac remodeling and with electrocardiographic abnormalities, and negatively with ejection fraction. CRP and ADA were higher in patients with cardiothoracic index ≥ 50%, while ADA was higher in patients with ventricular repolarization disturbances. Finally, CRP was positively correlated with ADA. In conclusion, ADA and CRP are prognostic markers of cardiac dysfunction and remodeling in Chagas disease.

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Section: Discussioncontrasting

confidence: 99%

Adenosine Deaminase Activity and Serum C-Reactive Protein as Prognostic Markers of Chagas Disease Severity

Bravo-Tobar

Nello-Pérez

Fernández

et al. 2015

Rev. Inst. Med. trop. S. Paulo

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“…The role of extracellular ATP was studied extensively especially when involved in neutrophil and platelet functions (32,33), whereas ATP released by apoptotic cells was shown to be critical in their identification and phagocytosis ("find me and remove me" signal) by macrophages and monocytes, in vitro (10). Here we hypothesized that ligation of CR1 by immune complex-loaded RBC would promote RBC ATP release, possibly as a first step in priming liver and spleen macrophages to remove CR1-adherent complement-opsonized microbeads or preparing the RBC membrane for the immune-transfer process.…”

Section: Discussionmentioning

confidence: 99%

CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process

Melhorn¹,

Brodsky²,

Estanislau³

et al. 2013

Journal of Biological Chemistry

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“…[92,93] Moreover, a recent study showed that the E-NPP activities increase in the platelets of patients with indeterminate form of Chagas' disease, indicating the role of these enzymes in protozoan pathogens invading the bloodstream. [94] By contrast, Vuaden et al, [95] demonstrated that 48 h of induced endotoxemia promotes a decrease in E-NPP activities in rat platelets and a reduction in platelet aggregation. Finally, Lopez-Castejón et al, [91] reported that the NPP-1 is highly expressed in M2 compared to M1 macrophages, while an exacerbated NPP-2 (autotaxin) and NPP-3 expression is associated with several types of cancer.…”

Section: Ecto-nucleoside Triphosphate Diphosphohydrolasesmentioning

confidence: 95%

The role of p2x7 receptor in infectious inflammatory diseases and the influence of ectonucleotidases

Morandini¹,

Savio²,

Coutinho‐Silva³

2014

Biomed J

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The purinergic receptor P2X ligand-gated ion channel 7 (P2X7) is ubiquitously expressed in almost all tissues and organs of the body with the highest distribution in the immune cells of monocyte-macrophage origin. Classically, P2X7 receptor is involved in apoptotic cell death, and it is well known that extracellular ATP ligation to this purinergic receptor serves as an important secondary stimulus, which is also considered as danger signal for the interleukin (IL)-1β cleavage and secretion from pro-inflammatory cells. More recently, however, there has been substantial evidence of additional roles for the P2X7 receptor, both in innate immune response and as an adaptive link, including T-cell activation in a chronic state of inflammation. Also, compelling evidences have revealed an important role for ectonucleotidases as ATP-consuming enzymes in the control and fine-tuning of P2X7 function by regulating the time, concentration, and availability of ATP during infection-driven inflammation. This review focuses on the current evidences for P2X7 receptor involvement in the initial stages of inflammation, as well as for its role in acute and chronic stages of infection. Here, we also highlight the role of ectonucleotidase family in the control of P2X7 function, including the initial and resolution phases of inflammation.

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Purinergic system ecto-enzymes participate in the thromboregulation of patients with indeterminate form of Chagas disease

Cited by 19 publications

References 49 publications

Adenosine Deaminase Activity and Serum C-Reactive Protein as Prognostic Markers of Chagas Disease Severity

Adenosine Deaminase Activity and Serum C-Reactive Protein as Prognostic Markers of Chagas Disease Severity

CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process

The role of p2x7 receptor in infectious inflammatory diseases and the influence of ectonucleotidases

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