Free and nanoencapsulated vitamin D<sub>3</sub>: effects on E‐NTPDase and E‐ADA activities in an animal model with induced arthritis

Silveira, Karine; Silveira, Leonardo Lanes da; Thorstenberg, Maria Luiza; Cabral, Fernanda Licker; Castilhos, Lívia Gelain; Rezer, João Felipe Peres; Andrade, Diego Fontana de; Beck, Ruy Carlos Ruver; Palma, Heloísa Einloft; Andrade, Cínthia Melazzo de; Pereira, Renata da Silva; Martins, Nara Maria Beck; Santos, Claudia de Mello Bertonchel dos; Leal, Daniela Bitencourt Rosa

doi:10.1002/cbf.3188

Cited by 12 publications

(6 citation statements)

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“…The only change in biochemical parameters indicative of liver dysfunction that was observed in our study was an increase in AST activity in the AS group. Corroborating our results, also reported increased AST levels in rats with induced arthritis, which may be a result of the arthritic inflammatory process.…”

Section: Discussionsupporting

confidence: 90%

Antioxidant, hepatoprotective, genoprotective, and cytoprotective effects of quercetin in a murine model of arthritis

Saccol

Silveira

Manzoni

et al. 2019

J of Cellular Biochemistry

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Rheumatoid arthritis is a highly debilitating inflammatory autoimmune disease which is characterized by joint destruction. The present study sought to investigate the effect of quercetin in rats with complete Freund's adjuvant‐induced arthritis. Animals were divided into control/saline, control/quercetin (5 mg/kg, 25 mg/kg, and 50 mg/kg) arthritis/saline, and arthritis/quercetin (5 mg/kg, 25 mg/kg, and 50 mg/kg); the treatments were administered for 45 days. Biochemical, oxidative stress, genotoxicity, and cytotoxicity parameters were evaluated. All doses of quercetin reduced the levels of aspartate aminotransferase, thiobarbituric acid‐reactive substances, and reactive oxygen species; however, only treatment with 25 or 50 mg/kg increased catalase activity. Total thiol and reduced glutathione levels were not significantly affected by the induction nor by the treatments. Genotoxicity assessed by DNA damage, and cytotoxicity through picogreen assay, decreased after treatments with quercetin. Our results present evidence of the antioxidant, cytoprotective, genoprotective and hepatoprotective, and effects of quercetin, demonstrating its potential as a candidate for coadjuvant therapy.

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Section: Discussionsupporting

confidence: 90%

Antioxidant, hepatoprotective, genoprotective, and cytoprotective effects of quercetin in a murine model of arthritis

Saccol

Silveira

Manzoni

et al. 2019

J of Cellular Biochemistry

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“…This has been found in lymphocytes from RA patients, in which increased extracellular NTPDase and reduced ADA activities have been reported [ 81 ]. Similar results were obtained using animals with complete Freund’s adjuvant-induced arthritis [ 82 , 83 ]. In agreement with these data, some studies have emphasized the role of e-ADA in inflammation, so that the serum activity of this enzyme is considered an inflammatory marker in diseases such as RA, based on the evidence that ADA, by causing a reduction in ADO levels, can stimulate the activity of immune cells, possibly also leading to bone dysfunctions [ 94 ].…”

Section: Role Of Purine Enzymes In Bone Inflammatory Conditionssupporting

confidence: 82%

In Search of a Role for Extracellular Purine Enzymes in Bone Function

et al. 2021

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Bone is one of the major tissues that undergoes continuous remodeling throughout life, thus ensuring both organic body growth during development and protection of internal organs as well as repair of trauma during adulthood. Many endogenous substances contribute to bone homeostasis, including purines. Their role has increasingly emerged in recent decades as compounds which, by interacting with specific receptors, can help determine adequate responses of bone cells to physiological or pathological stimuli. Equally, it is recognized that the activity of purines is closely dependent on their interconversion or metabolic degradation ensured by a series of enzymes present at extracellular level as predominantly bound to the cell membrane or, also, as soluble isoforms. While the effects of purines mediated by their receptor interactions have sufficiently, even though not entirely, been characterized in many tissues including bone, those promoted by the extracellular enzymes providing for purine metabolism have not been. In this review, we will try to circumstantiate the presence and the role of these enzymes in bone to define their close relationship with purine activities in maintaining bone homeostasis in normal or pathological conditions.

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“…At the 0, 14th, and 21st day, a vernier caliper was used to measure the thickness of toes. From the 10th to 21st day, the arthritis index (AI) was evaluated according to the degree of joint swelling (da Silveira et al, 2016). The other six rats, as the control group, were given the same treatment but with the normal saline used as a replacement for the CII.…”

Section: Establishment Of Rat Model Of Collagen-induced Arthritis (Cia)mentioning

confidence: 99%

“…was evaluated according to the degree of joint swelling (da Silveira et al, 2016). The other six rats, as the control group, were given the same treatment but with the normal saline used as a replacement for the CII.…”

Section: Establishment Of Rat Model Of Collagen-induced Arthritis (Cia)mentioning

confidence: 99%

RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll‐like receptor signaling pathway

Tang

Zeng

et al. 2019

Journal Cellular Physiology

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Emerging evidence shows that rheumatoid arthritis (RA) progression can be induced by the activation of Toll-like receptor (TLR) signaling pathway. Regulator of G-protein signaling 1 (RGS1) is observed to be a candidate biomarker for arthritis. Accordingly, the present study aims to determine the potential effects of RGS1 mediating TLR on RA. A rat model of collagen-induced arthritis (CIA) was established to mimic the features of RA by injection of bovine type II collagen. The rats with CIA were treated with short hairpin RNA (shRNA) against RGS1 or TLR pathway activator Poly I:C to elucidate the role of RGS1 in RA progression. The inflammatory factors were measured, and the thoracic gland and spleen indexes as well as the vascular density were determined. The expression levels of RGS1, TLR3, vascular endothelial growth factor (VEGF), metalloproteinase-2 (MMP-2), MMP-9, and interleukin 1 receptorassociated kinase-4 (IRAK4) were determined. RGS1 was robustly increased in RA.The TLR signaling pathway was suppressed by RGS1 silencing. shRNA-mediated depletion of RGS1 was shown to significantly enhance thoracic gland index and inhibit the serum levels of TNF-α, IL-1β, and IL-17, spleen index, vascular density, and the expression levels of TLR3, VEGF, MMP-2, MMP-9, and IRAK4. However, when the rats with CIA were treated with Poly I:C, the trend of effects was opposite. These findings highlight that functional suppression of RGS1 inhibits the inflammatory response and angiogenesis by inactivating the TLR signaling pathway in rats with CIA,

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Free and nanoencapsulated vitamin D₃: effects on E‐NTPDase and E‐ADA activities in an animal model with induced arthritis

Cited by 12 publications

References 50 publications

Antioxidant, hepatoprotective, genoprotective, and cytoprotective effects of quercetin in a murine model of arthritis

Antioxidant, hepatoprotective, genoprotective, and cytoprotective effects of quercetin in a murine model of arthritis

In Search of a Role for Extracellular Purine Enzymes in Bone Function

RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll‐like receptor signaling pathway

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Free and nanoencapsulated vitamin D3: effects on E‐NTPDase and E‐ADA activities in an animal model with induced arthritis

Cited by 12 publications

References 50 publications

Antioxidant, hepatoprotective, genoprotective, and cytoprotective effects of quercetin in a murine model of arthritis

Antioxidant, hepatoprotective, genoprotective, and cytoprotective effects of quercetin in a murine model of arthritis

In Search of a Role for Extracellular Purine Enzymes in Bone Function

RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll‐like receptor signaling pathway

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Free and nanoencapsulated vitamin D₃: effects on E‐NTPDase and E‐ADA activities in an animal model with induced arthritis