Activation Patterns of Murine T Cells after Oral Administration of an Enterocoated Soluble Antigen

Jain, Shilpa L.; Barone, K.Siobhan; Michael, J.Gabriel

doi:10.1006/cimm.1996.0024

Cited by 20 publications

(6 citation statements)

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“…have previously shown that low doses of orally administered αs1-casein, a major milk allergen, provoke IFN-γ secretion by splenocytes and IgG2a production in C3H/He mice (Yoshida et al, 1997). Some other investigators have also reported that an orally administered antigen induced IFN-γ secretion (Hoyne and Thomas, 1995;Jain et al, 1996;Sun et al, 1999), although in these reports the dose of antigen fed was not consistent. Although our results could not demonstrate the upregulation of IFN-γ , it is possible that small amounts of IFN-γ secreting cells with effector functions were induced in mice fed the intermediate (5 mg) dose, resulting in Th1-type responses.…”

Section: Discussionmentioning

confidence: 97%

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et al. 2000

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Although oral administration of a soluble proteinantigen can induce various immune responses, theeffect of the dosage of oral antigen on thepredominance of Th2-type cytokine and antibodyresponses has not been well clarified yet. In thepresent study, we fed T cell receptor (TCR) transgenic(tg) mice various amounts of chicken ovalbumin (0.1,5, and 250 mg) and examined the resulting immuneresponses to this antigen. In these TCR tg mice, theresponses of antigen-specific T cells were greatlyamplified concomitantly with significantantigen-specific cytokine secretion. We found that ahigh dose (250 mg) of antigen significantlyupregulated the serum antigen-specific IgG1 and IgAantibody responses in mice later intraperitoneallyinjected with antigen plus adjuvant. The miceadministered the same oral dose but not immunizedshowed upregulation of Th2-type IL-4 and IL-5secretion and downregulation of Th1-type IL-2 andIFN-gamma. This enhancement of Th2-type cytokineand antibody responses was more marked when largerdoses of antigen orally administered. These resultsdemonstrated that antigen feeding induces thedevelopment of T cells secreting Th2-type cytokines ina dose-dependent manner and that these T cells have ahelper function for the production of antibodies ofthe Th2-type isotypes. This experimental system shouldbe useful to screen foods and other substances thatcan modulate Th2-type responses relating to allergy.

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Section: Discussionmentioning

confidence: 97%

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et al. 2000

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“…The production of anti‐CII IgG1 antibodies that needs the help of Th2 cells (Boom et al , 1988) was markedly reduced by treatment with 11B11 mAb. Conversely, the mAb treatment was effective in enhancing anti‐CII IgG2a production which is dependent on Th1 cells (Jain et al , 1996). These results appear to be supported by previous findings, that IL‐4 augments Th2 cell‐dependent antibody production, but suppresses Th1 cell‐dependent antibody production (Finkelman et al , 1988).…”

Section: Discussionmentioning

confidence: 99%

Effect of a monoclonal antibody against interleukin‐4 on collagen‐induced arthritis in mice

Yoshino

1998

British J Pharmacology

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1 The present study was undertaken to investigate the eect of a monoclonal antibody (11B11 mAb) against interleukin-4 (IL-4) on collagen-induced arthritis (CIA) in mice. 2 11B11 mAb was daily injected intraperitoneally over a period of 10 days, commencing on the day of immunization with type II collagen (CII).3 The results showed that the anti-IL-4 mAb markedly augmented both the incidence and the severity of CIA. The augmentation of the disease was associated with a signi®cant increase in anti-CII IgG2a antibody production, proliferative responses of lymph node cells to CII and interferon-g (IFN-g) secretion from the lymphoid cells. The production of anti-CII IgG1 antibodies the secretion of IL-4 was markedly reduced in the mAb-treated mice. 4 Thus, the neutralization of IL-4 by 11B11 mAb appears to be eective in augmenting CIA.

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“…When mice were fed the soluble protein ovalbumin, their antigen-specific splenic T cells were unresponsive and secreted little, if any, IL-4 and IFN-g, but they showed antigen dose-dependent T-cell proliferation and secretion of high levels of IL-4 when fed the same antigen in an encapsulated form. 75 This could, however, be due to the protection from acid and enzymatic degradation of the protein through encapsulation, as detailed above.…”

Section: Form Of the Antigenmentioning

confidence: 99%