Activation of porcine oocytes using cyclopiazonic acid, an inhibitor of calcium-dependent ATPases

Petr, Jaroslav; Rozinek, J.; Jílek, F.; Urbánková, Dita

doi:10.1002/1097-010x(20000901)287:4<304::aid-jez5>3.0.co;2-6

Cited by 11 publications

(6 citation statements)

References 74 publications

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“…The intracellular calcium stores in the endoplasmic reticulum of mouse oocytes play an important role during oocyte activation (Swann & Lai, 1997;Mohri et al, 2001). In pig oocytes, the inhibition of calciumdependent ATPases is able to induce parthenogenetic activation (Petr et al, 2000). On the other hand, it is not clear whether endoplasmic reticulum is the target for this treatment of pig oocytes, because the endoplasmic reticulum is not abundant in pig oocytes and other organelles are an important source of intracellular calcium (e.g.…”

Section: Discussionmentioning

confidence: 99%

Nitric-oxide-dependent activation of pig oocytes: the role of the cGMP-signalling pathway

Petr

Rajmon

Eva

et al. 2006

Zygote

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Pig oocytes matured in vitro were parthenogenetically activated (78%) after treatment with 2 mM nitric oxide-donor (+/-)-S-nitroso-N-acetylpenicillamine (SNAP) for 24 h. Inhibition of soluble guanylyl cyclase with the specific inhibitors 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) or 6-anilino-5,8-quinolinequinone (LY83583) suppressed the SNAP-induced activation in a dose-dependent manner (23% of activated oocytes after treatment with 400 microM ODQ; 12% of activated oocytes after treatment with 40 microM LY83583). 8-Bromo-cyclic guanosine monophosphate (8-Br-cGMP), a phosphodiesterase-resistant analogue of cGMP, enhances the effect of suboptimal doses (0.1 or 0.5 mM) of the NO donor SNAP. DT3, a specific inhibitor of cGMP-dependent protein kinase (PKG, PKG), is also able to inhibit the activation of pig oocytes after NO donor treatment. Involvement of the cGMP-dependent signalling pathway is specific for NO-induced oocyte activation, because both the guanylyl cyclase inhibitor ODQ and the PKG inhibitor DT3 are unable to inhibit activation in oocytes treated with the calcium ionophore A23187. These data indicate that the activation of pig oocytes with an NO donor is cGMP-dependent and that PKG plays an important role in this mode of oocyte activation.

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Section: Discussionmentioning

confidence: 99%

Nitric-oxide-dependent activation of pig oocytes: the role of the cGMP-signalling pathway

Petr

Rajmon

Eva

et al. 2006

Zygote

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“…The ratio of aged oocytes spontaneously activated in vitro is generally low (with maxima up to 45%) and is lower than the activation rate a�er the artificial parthenogenesis with various artificial stimuli in pig oocytes matured in vitro under our culture conditions (Jílek et al , 2001Petr et al, 1995Petr et al, , 2000Petr et al, , 2002. Both diploid parthenogenetic embryos (i.e.…”

Section: Discussionmentioning

confidence: 82%

In vitro aging of porcine oocytes

Petrova¹,

Sedmı́ková²,

Eva³

et al. 2004

CZECH J ANIM SCI

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Porcine oocytes matured in vitro develop in various ways if they are further cultivated. In our studies these oocytes were cultivated for 1 to 5 days (in vitro aging). During the 1st day of aging, most of them remained at the stage of metaphase II (98%). Then many oocytes underwent the spontaneous parthenogenetic activation. The portion of activated oocytes reached its peak a�er 2 or 3 days of aging in vitro (39 or 45%). The portion of fragmented oocytes peaked at the same time (28%). During subsequent aging in vitro (i.e. day 4 or 5 of aging), the portion of lysed oocytes significantly increased (30 or 37%). The highest portion of spontaneously activated parthenogenetic embryos at a pronuclear stage (35%) was observed during the 2nd day of aging in vitro. These pronuclear embryos had mainly one polar body with two pronuclei (47% of all pronuclear embryos) or two polar bodies with one pronucleus (38% of all pronuclear embryos). During the 3rd and 5th day of in vitro aging, there was a significant increase in the portion of parthenogenetic embryos cleaved to the 2-cell or 3-cell stage. When considering the prolonged in vitro culture of porcine oocyte, only the first day of aging should be taken into account, since beyond this time significant changes, i.e. parthenogenesis, fragmentation or lysis, occurred in oocytes under in vitro conditions.

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“…It is possible that activation rates may be further improved through the use of alternative calcium ionophores, such as A23187 [58], [59], or by inhibitors of Ca 2+ -dependent ATPases ( e.g. , thapsigargin [60]). …”

Section: Discussionmentioning

confidence: 99%

Efficient Activation of Reconstructed Rat Embryos by Cyclin-Dependent Kinase Inhibitors

et al. 2010

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BackgroundOver the last decade a number of species, from farm animals to rodents, have been cloned using somatic cell nuclear transfer technology (SCNT). This technique has the potential to revolutionize the way that genetically modified animals are made. In its current state, the process of SCNT is very inefficient (<5% success rate), with several technical and biological hurdles hindering development. Yet, SCNT provides investigators with powerful advantages over other approaches, such as allowing for prescreening for the desired level of transgene expression and eliminating the excess production of undesirable wild-type animals. The rat plays a significant role in biomedical research, but SCNT has been problematic for this species. In this study, we address one aspect of the problem by evaluating methods of activation in artificially constructed rat embryos.Principal FindingsWe demonstrate that treatment with a calcium ionophore (ionomycin) combined with a variety of cyclin-dependent kinase inhibitors is an effective way to activate rat embryos. This is in contrast to methods developed for the mouse embryo, which tolerates much less specific chemical treatments. Methods developed to activate mouse embryos do not translate well to rat embryos.ConclusionsActivation methods developed for one species will not necessarily translate to another species, even if it is closely related. Further, the parthenogenic response to chemical activators is not always a reliable indicator of how reconstructed embryos will react to the same activation method. A better understanding of rat oocyte physiology, although essential for developing better models of disease, may also provide insights that will be useful for making the SCNT process more efficient.

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Activation of porcine oocytes using cyclopiazonic acid, an inhibitor of calcium-dependent ATPases

Cited by 11 publications

References 74 publications

Nitric-oxide-dependent activation of pig oocytes: the role of the cGMP-signalling pathway

Nitric-oxide-dependent activation of pig oocytes: the role of the cGMP-signalling pathway

In vitro aging of porcine oocytes

Efficient Activation of Reconstructed Rat Embryos by Cyclin-Dependent Kinase Inhibitors

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