2010
DOI: 10.1371/journal.pone.0009799
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Efficient Activation of Reconstructed Rat Embryos by Cyclin-Dependent Kinase Inhibitors

Abstract: BackgroundOver the last decade a number of species, from farm animals to rodents, have been cloned using somatic cell nuclear transfer technology (SCNT). This technique has the potential to revolutionize the way that genetically modified animals are made. In its current state, the process of SCNT is very inefficient (<5% success rate), with several technical and biological hurdles hindering development. Yet, SCNT provides investigators with powerful advantages over other approaches, such as allowing for prescr… Show more

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Cited by 4 publications
(1 citation statement)
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“…It is believed that premature chromosome condensation (PCC) activity of the nonactivated cytoplasm contributes to nuclear reprogramming and improves development of NT embryos. However, other groups had difficulties reproducing the achievement, perhaps because of inherited developmental toxicity and strain dependency of this particular drug (Mizumoto et al, 2008(Mizumoto et al, , 2010Nakajima et al, 2008;Tomioka et al, 2007;Webb et al, 2010). In search of alternatives, we discovered that the activation of rat oocytes could be reversibly suppressed with microtubule and microfilament disruptive agents such as cytochalasin B, cytochalasin D, nocodazole, and demecolcyne (Galat et al, 2007).…”
Section: Lessons and Perspectives From Cloningmentioning
confidence: 99%
“…It is believed that premature chromosome condensation (PCC) activity of the nonactivated cytoplasm contributes to nuclear reprogramming and improves development of NT embryos. However, other groups had difficulties reproducing the achievement, perhaps because of inherited developmental toxicity and strain dependency of this particular drug (Mizumoto et al, 2008(Mizumoto et al, , 2010Nakajima et al, 2008;Tomioka et al, 2007;Webb et al, 2010). In search of alternatives, we discovered that the activation of rat oocytes could be reversibly suppressed with microtubule and microfilament disruptive agents such as cytochalasin B, cytochalasin D, nocodazole, and demecolcyne (Galat et al, 2007).…”
Section: Lessons and Perspectives From Cloningmentioning
confidence: 99%