The Laboratory Rat 2020
DOI: 10.1016/b978-0-12-814338-4.00007-6
|View full text |Cite
|
Sign up to set email alerts
|

Assisted Reproductive Technologies and Genetic Modifications in Rats

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
10
0
2

Year Published

2021
2021
2024
2024

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 9 publications
(13 citation statements)
references
References 219 publications
0
10
0
2
Order By: Relevance
“…Then, fertilization was checked, and the fertilized oocytes were washed and transferred to culture drops, and the resulting embryos were monitored up to the blastocyst stage. 20 Before the oocyte collection, a mixture of 75 mg/kg of ketamine (Ketasol, Richter Pharma, Austria) and 10 mg/kg of xylazine (Basilazin, Bavet, Turkey) was applied intraperitoneally to a male rat, and then the rat was euthanized. Following the euthanasia procedure, the male reproductive system was surgically opened from the abdomen, and the left and right epididymis were separated from the testicles and transferred to HTF medium containing 1 ml of HTF (Cat.…”
Section: Methodsmentioning
confidence: 99%
“…Then, fertilization was checked, and the fertilized oocytes were washed and transferred to culture drops, and the resulting embryos were monitored up to the blastocyst stage. 20 Before the oocyte collection, a mixture of 75 mg/kg of ketamine (Ketasol, Richter Pharma, Austria) and 10 mg/kg of xylazine (Basilazin, Bavet, Turkey) was applied intraperitoneally to a male rat, and then the rat was euthanized. Following the euthanasia procedure, the male reproductive system was surgically opened from the abdomen, and the left and right epididymis were separated from the testicles and transferred to HTF medium containing 1 ml of HTF (Cat.…”
Section: Methodsmentioning
confidence: 99%
“…For ovarian stimulation, 150 to 300 IU/kg of pregnant mare serum gonadotropin (Chronogest/PMSG, Intervet, Istanbul, Turkey) was administered using an intraperitoneal injection, followed by 150 to 300 IU/kg of human chorionic gonadotropin (hCG; Gonatropin, Chorulon Intervet, Istanbul, Turkey) approximately 48 h later 18,19 . A 15 IU dose of pregnant mare serum gonadotropin was administered 17 to 19 h after hCG administration 20 . All the rats were weighed and anesthetized by an intramuscular administration of 50 mg/kg of ketamine hydrochloride (Ketalar; Eczacibasi Warner‐Lambert Ilac Sanayi, Levent, Istanbul, Turkey) and 7 mg/kg xylazine hydrochloride (Rompun, Bayer Sisli, Istanbul, Turkey).…”
Section: Methodsmentioning
confidence: 99%
“…Then, fertilization was checked and the fertilized oocytes were washed and transferred to culture drops, and the resulting embryos were monitored up to transfer 30 . The total number of cells was counted as an embryo evaluation criterion: the ratio of blastomeres size and fragmentation rates, as well as the number of blastomeres, were used as described by Ahumada 31 .…”
Section: Methodsmentioning
confidence: 99%