Assisted Reproductive Technologies and Genetic Modifications in Rats

Rev Bras Ginecol Obstet

Toprak³

et al. 2022

Objective Premature ovarian insufficiency (POI) contributes significantly to female infertility. Cyclophosphamide (CYC has adverse effects on folliculogenesis. Platelet-rich plasma (PRP) is an autologous product rich in many growth factors. We evaluated the protective effect of PRP on in vitro fertilization in female rats with CYC-induced ovarian damage. Methods Twenty-eight adult female Sprague-Dawley rats were randomly divided into four groups. Group 1 (control-sodium chloride 0.9%; 1 mL/kg, single-dose intraperitoneal [IP] injection); group 2 (CYC), 75 mg/kg, single-dose IP injection and sodium chloride 0.9% (1 mL/kg, single-dose IP injection); group 3 CYC plus PRP, CYC (75 mg/kg, single-dose and PRP (200 μl, single-dose) IP injection); and group 4 (PRP, 200 μl, single-dose IP injection). Results In the comparisons in terms of M1 and M2 oocytes, it was observed that the CYC group presented a significantly lower amount than the control, CYC/PRP, and PRP groups. (for M1, p = 0.000, p = 0.029, p = 0.025; for M2, p = 0.009, p = 0.004, p = 0.000, respectively). The number of fertilized oocytes and two-celled good quality embryos was found to be statistically significant between the CYC and control groups, CYC + PRP and PRP groups (p = 0.009, p = 0.001, p = 0.000 for oocytes, respectively. For embryos; p = 0.016, p = 0.002, p = 0.000). Conclusion Platelet-rich plasma can protect the ovarian function against damage caused by CYC, and, in addition, it improves oocyte count and the development of embryos as a result of oocyte stimulation during the IVF procedure.

Section: Methodsmentioning

confidence: 99%

Protective Effects of Platelet-rich plasma for in vitro Fertilization of Rats with Ovarian Failure Induced by Cyclophosphamide

Rev Bras Ginecol Obstet

Toprak³

et al. 2022

“…For ovarian stimulation, 150 to 300 IU/kg of pregnant mare serum gonadotropin (Chronogest/PMSG, Intervet, Istanbul, Turkey) was administered using an intraperitoneal injection, followed by 150 to 300 IU/kg of human chorionic gonadotropin (hCG; Gonatropin, Chorulon Intervet, Istanbul, Turkey) approximately 48 h later 18,19 . A 15 IU dose of pregnant mare serum gonadotropin was administered 17 to 19 h after hCG administration 20 . All the rats were weighed and anesthetized by an intramuscular administration of 50 mg/kg of ketamine hydrochloride (Ketalar; Eczacibasi Warner‐Lambert Ilac Sanayi, Levent, Istanbul, Turkey) and 7 mg/kg xylazine hydrochloride (Rompun, Bayer Sisli, Istanbul, Turkey).…”

Section: Methodsmentioning

confidence: 99%

The effect of protection of platelet‐rich plasma against experimental ischemia/reperfusion injury in the rat ovary on in vitro fertilization outcomes

J of Obstet and Gynaecol

Çakıroğlu

et al. 2022

Objective Ovarian torsion is a common cause of local ischemic damage, reduced follicular activity and infertility. This study aimed to investigate how well platelet‐rich plasma (PRP) protects against experimental ischemic (I) and ischemia‐reperfusion (I/R) injury in rat ovaries and its effect on in vitro fertilization (IVF) outcomes. Method Fifty‐six adult female Sprague–Dawley albino rats were randomly assigned to six groups of eight animals each: Sham, Ischemia, I/R, Sham + PRP, I + PRP, and I/R + PRP. The remaining eight animals were used to prepare the PRP. The ischemia groups were subjected to bilateral adnexal torsion for 3 h, while the I/R and I/R + PRP groups received subsequent detorsion for 3 h. Intraperitoneal (i.p.) PRP was administered 30 min prior to ischemia (I + PRP) or reperfusion (I/R + PRP). The ovaries were stimulated through an intraperitoneal injection of 150–300 internal units of IU/kg PMSG. After ovulation induction, oocytes were taken from the ovaries, and IVF was performed. Results The number of MII oocytes reached the highest number with 4.63 ± 0.74 in the S group and had the lowest number with 0.50 ± 0.53 in the I/R group. There were statistically significant differences for the number of embryos obtained on the second day between the I and I + PRP groups and the I/R and I/R + PRP groups (p = 0.000). In comparing anti‐Müllerian hormone 1 (AMH1) and AMH2 values within the group, the highest decrease was observed in the I and I/R groups. Conclusion PRP is effective in minimizing ovarian damage and preserving ovarian reserves following ovarian torsion.

“…Then, fertilization was checked and the fertilized oocytes were washed and transferred to culture drops, and the resulting embryos were monitored up to transfer 30 . The total number of cells was counted as an embryo evaluation criterion: the ratio of blastomeres size and fragmentation rates, as well as the number of blastomeres, were used as described by Ahumada 31 .…”

Section: Methodsmentioning

confidence: 99%

The effect of Propofol versus Dexmedetomidine as anesthetic agents for oocyte pick-up on in vitro fertilization (IVF) outcomes

Tuna

et al. 2021

Sci Rep

This study aimed to evaluate the effects of propofol and dexmedetomidine over different timescales on the IVF outcomes for transvaginal oocyte retrieval (TVOR). Twenty-four rats included in the study were divided into two main groups and three subgroups were subjected to the ovulation induction process. Group 1 was administered propofol (100 mg/kg i.v.) and group 2 were administered dexmedetomidine (25 µg/kg i.p.) The oviduct collection procedure was completed within 15 min for subgroup Pro15min, Dex15min (n = 4), within 16 to 30 min for subgroup Pro30min, Dex30min (n = 4) and within 31 to 60 min for subgroup Pro60min, Dex60min (n = 4) after euthanasia. The total number of oocytes was counted. After in vitro fertilization, the number and quality of embryos were evaluated. The number of pups born were evaluated after embryo transfer. The embryo number, quality and pup count decreased as the administration time for propofol increased (p < 0.05). No statistically significant difference was found between the dexmedetomidine subgroups for embryo number, quality and pup count(p > 0.05). As the exposure time to propofol increased, the number and quality of embryos obtained, and the pup count, decreased. The use of dexmedetomidine had no negative impacts on the number of embryos, their quality or the number of pups.