Activation of ERK-like MAP kinase involved in regulating the cellular proliferation and differentiation of immobilized Taxus cuspidata cells

Spatial–temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and Taxol production in immobilized Taxus cuspidata cells

“…1-3). Taxol content in immobilized support matrices also increased from outer zone to central zone on days 20 and 25 (Cheng et al, 2006;Cheng and Yuan, 2006a). Additionally, the NO levels of both total and central zone in immobilized support matrices reached the highest on day 20 (Fig.…”

“…Previous results revealed that extracellular signal-regulated kinase-like (ERK-like) mitogen-activated protein kinase (MAPKs) and arabinogalactan proteins were involved in cellular proliferation, differentiation, and Taxol production of immobilized T. cuspidata cells (Cheng et al, 2006(Cheng et al, , 2008. Cheng and Yuan also revealed that the expression levels of some proteins displayed obviously spatial heterogeneity in immobilized cells (Cheng and Yuan, 2006a).…”

RGD-Dependent Mechanotransduction of Suspension Cultured Taxus Cell in Response to Shear Stress

“…Non‐RGD peptides GRGES was used as control for RGD due to the structural similarity of amino acid D (Asp) and E (Glu). Several different events of shear stress signal transduction and response have been identified in T. cuspidata , including alkalinization of the culture medium, generation of ROS, accumulation of phenolics (5), and activation of MAPK‐like cascades (17, 18). So these events were chosen for RGD competition assays.…”

“…In plants, though proteins resembling immunochemically to the mammalian dually phosphorylated p38‐MAPK (19) and JNK‐MAPK (20) were detected, based on sequence analysis, the presently known plant MAPKs appear to belong to the ERK subgroup, and increasing evidence indicates that these kinases are also involved in various forms of biotic and abiotic stress responses. Our previous experiment (18) has shown that two protein bands with molecular weights of 44 and 46 kDa in Taxus cells could be detected by a p‐ERK antibody of human origin in Western blot assays, and their activity was reduced by specific inhibitor of MEK (PD98059). An in‐gel kinase assay has shown that the MBP substrate could be activated by a protein kinase of relative molecular mass 46 kDa, and the activity of this protein kinase changed with kinetics similar to that of the 46 kDa protein detected by mammalian p‐ERK antibody.…”

Enhancement of biohydrogen production from starch processing wastewater and further inside its ecosystem disclosed by 16S rDNA sequencing and FISH

“…Iron could improve hydrogenase enzyme activity, which involve in hydrogen formation, as a component on active site of the enzyme [3] . Immobilization of bacteria on supporting materials helps improve hydrogen productivity by acclimatization of microbes, decreasing lag phase of bacterial cultivation [4] , and increasing density of consortia [5] . There were many reports of using synthetic materials but fewer publications studied about biological supporting materials (BM).…”