Activation of Endogenous Hydrogen Sulfide Synthesis Inhibits Mitochondrial Permeability Transition Pore Opening and Restores Constitutive No-Synthase Coupling in Old Rat Heart

Mys, Lidiia; Strutyns'ka, NA; Strutynskyi, V.R.; Сагач, В. Ф.

doi:10.15407/fz63.03.016

Cited by 4 publications

(3 citation statements)

References 17 publications

(25 reference statements)

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“…Statistical calculations were performed using the "StatisticSoft 6.0" program. [19]. The concentration of peroxynitrite rose by 55.5%.…”

Section: Methodsmentioning

confidence: 95%

“…It is considered that any increase in О 2 production (by mitochondria, xanthine oxidase, or NADPH-oxidase) leads to cNOS uncoupling. This cNOS becomes not only a powerful О 2 generator by itself, but at the same time it activates other sources of О 2 - [19]. Peroxynitrite concentration was measured by using its reaction with potassium iodide under pH 7.0 in 0.2 M phosphate buffer with the same pH [17].…”

Section: Methodsmentioning

confidence: 99%

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Quercetin Limits the Progression of Oxidative and Nitrosative Stress in the Rats’ Tissues After Experimental Traumatic Brain Injury

et al. 2020

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The aim: To investigate the effect of water-soluble form of quercetin on the indices reflecting the progression of oxidative-nitrosative stress in the cerebral tissues and the periodontium of rats after experimental TBI. Materials and methods: The studies were conducted on 30 white rats of the Wistar line weighing 180-220 g, divided into 3 groups: the 1st group included pseudo-injured animals (subjected to ether anaesthesia, fixation without TBI modeling), the 2nd group included the animals exposed to modeled moderate TBI, the 3rd group involved the rats, which were given injections with water-soluble form of quercetin (corvitin, “Borshchahivskiy CPP”, Ukraine) intraperitoneally in a daily dose of 10 mg/kg recalculated for quercetin for 7 days following the TBI modeling. The formation of superoxide radical anion (.О2 -), activity of NO-synthase – total (NOS), its constitutive and inducible isoforms (cNOS, iNOS) – and concentration of peroxynitrite were evaluated spectrophotometrically. The level of lipid peroxidation (LPO) in the tissues was evaluated by the formationof a stained trimethine complex during the reaction of tiobarbituric acid (TBA). The activity of the antioxidant system was assessed by increasing in the concentration of TBA active products during 1.5 hour incubation in iron-ascorbate buffer solution, as well as by the activity of antioxidant enzymes – superoxide dismutase (SOD) and catalase. Results: The use of quercetin under the experimental conditions significantly reduced the О2 - generation by NADPH- and NADH-dependent electron transport chains by 30.2 and 35.0% (in the cerebral hemispheres) and by 23.5 and 32.5% (in the soft periodontal tissues), respectively, compared to the findings in the 2nd group. The production of this radical by leukocyte NADPH oxidase in these organs was inferior to the value of the 2nd group by 39.3 and 29.9%. We revealed that the use of quercetin in the experimental conditions probably reduced the activity of NOS, including iNOS, by 38.2 and 45.3% (in the cerebral hemispheres) and by 53.5 and 66.9% (in the soft periodontal tissues), respectively, compared with the findings in the 2nd group. Under these conditions, the cNOS activity went up by 50.0% and doubled, the peroxynitrite content was lower by 19.5 and 32.1% than that in the 2nd group. The administration of quercetin in the experimental conditions significantly reduced the concentration of TBA-active products in the homogenate of cerebral hemispheres and soft periodontal tissues. The development of decompensated LPO is also confirmed by a decrease in the activity of SOD and catalase. Conclusions: on the 7th day after modeling moderate TBI in rats the signs of oxidative-nitrosative stress are found not only in locus morbi (in the tissue of the cerebral hemisphere), but also in distant organs (periodontal tissues). Applying of water-soluble form of quercetin significantly reduces signs of oxidative-nitrosative stress in the tissue of the cerebral hemisphere of rats, as well as in periodontal tissues on the 7th day after moderate TBI modeling.

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“…Statistical calculations were performed using the "StatisticSoft 6.0" program. [19]. The concentration of peroxynitrite rose by 55.5%.…”

Section: Methodsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Quercetin Limits the Progression of Oxidative and Nitrosative Stress in the Rats’ Tissues After Experimental Traumatic Brain Injury

et al. 2020

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“…Increased vascular tone and increased resistance of the vascular wall to blood fl ow with age may occur due to impaired synthesis of vasodilator molecules, in particular such as Nitric Oxide (NO) and hydrogen sulfi de (H 2 S). It has been shown that under oxidative stress, which progresses with hypertension and aging, the production of gas transmitters is signifi cantly reduced [10][11][12]. Therefore, in the work aimed at the treatment or prevention of these diseases, decrease of markers of oxidative stress and restoration of glutathione levels is considered a promising target.…”

Section: Introductionmentioning

confidence: 99%

Garlic supplement lowers blood pressure in 40-60 years old hypertensive individuals, regulates oxidative stress, plasma cholesterol and protrombin index

Kravchuk¹,

Goshovska²,

Korkach³

et al. 2021

J Cardiovasc Med Cardiol

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Background: Arterial hypertension is associated with tissue remodeling, oxidative stress and decreased production of such vasorelaxants as nitric oxide (NO) and hydrogen sulfi de (H 2 S). Garlic shows promises in treatment of hypertension and could be used as an antioxidative agent and a source/precursor of H 2 S for decreasing of blood vessel stiffness. Objective:The aim was to study the effectiveness of garlic supplement in the treatment of grade II hypertension in male patients. Methods:The study was carried out in Kozyatyn Central District Hospital (Kozyatyn, Vinnytsia region, Ukraine). Patients with grade II hypertension (40-60 years old men) and healthy individuals of the same age and gender were included into study (n = 10 in each group). Garlic supplement (400mg per day) was included to the treatment after 10 day of standard antihypertensive therapy (angiotensin converting enzyme inhibitors, beta blockers). Echocardiography, biochemical control of blood, determination of plasma H 2 S level and markers of oxidative-nitrozative stress were performed during study period.Results: It was found that 30 days of garlic supplement on the background of a standard antihypertensive therapy reduced both systolic and diastolic blood pressure by 16.5 and 12.5 mmHg respectively (P<0,01 for both). The plasma H 2 S level was signifi cantly increased after 30 days of treatment. There was a positive effect of garlic supplement consumption on blood vessels in the form of a decrease in total peripheral vascular resistance by 10% (P<0,01). The production of reactive oxygen species and malonic dialdehyde was signifi cantly reduced in blood plasma indicating antioxidative effect of garlic. Importantly, activity of constitutive NO synthase was restored and inducible NO synthase activity was inhibited. There was a decrease in plasma cholesterol by 30% and prothrombin index (P<0,001 for both). No signifi cant changes in urea, creatinine, glucose, and erythrocyte counts were observed. Conclusion:The results of our study indicate the effectiveness of garlic supplement consumption as a hypotensive, cholesterol-lowering and mild antithrombotic agent in the treatment of hypertension in elderly patients. Garlic supplement consumption likely improves H 2 S and NO synthesis in hypertensive patients.

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Sex characteristics of hydrogen sulfide influence on ischemiareperfsion in miocardium of rats

Melnyk¹,

Заічко²,

Khodakovskyi³

et al. 2018

Fiziol Zh

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Досліджено зміни показників кардіогемодинаміки на тлі ішемії-реперфузії у 40 самців та самиць білих щурів та їх зв'язок з вмістом сірководню (Н 2 S) в сироватці крові. На моделі ішемії-реперфузії оцінені параметри серцевої гемодинаміки (коефіцієнт мікроциркуляції, насичення киснем лівого шлуночка, тиск у правому та лівому шлуночках) та вміст сірководню в сироватці крові. Встановлено, що у самців вихідні значення коефіцієнта мікроциркуляції лівого шлуночка та вміст Н 2 S у сироватці крові вірогідно менші (на 9-18%), ніж у самиць. За умов експериментальної оклюзії лівої коронарної артерії реєстрували зменшення вмісту Н 2 S в сироватці крові (у самців-на 64,5%, у самиць-на 51,1%), що супроводжувалося пригніченням мікроциркуляції та кисневого забезпечення кардіоміоцитів лівого шлуночка (зменшувалися коефіцієнт мікроциркуляції та сатурація киснем), послабленням його скоротливої здатності та порушенням внутрішньошлуночкової гемодинаміки (знижувався тиск у лівому та правому шлуночках). Вказані зміни були більш істотними у самців, ніж у самиць. Реперфузія вінцевої судини у тварин обох статей не викликала повного відновлення вмісту Н 2 S в сироватці крові та показників кардіогемодинаміки до вихідних значень, що свідчить про формування синдрому невідновленого вінцевого кровообігу. Введення NaHS не тільки перешкоджало розвитку ішемії серцевого м'яза, а й сприяло більш повноцінній реканалізації за рахунок покращення ефективної перфузії як у самців, так і у самиць. Вміст Н 2 S в сироватці крові виявляв прямі кореляційні зв'язки з коефіцієнтом мікроциркуляції, сатурацією кисню лівого шлуночка, тиском у правому та лівому шлуночках (у самців r=0,54-0,62; у самиць r=0,60-0,70). Отримані результати свідчать про важливу роль системи Н 2 S у регуляції мікроциркуляції та кардіогемодинаміки у самців та самиць щурів на тлі ішемії-реперфузії. Ключові слова: сірководень; кардіогемодинаміка; ішемія-реперфузія; стать.

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Activation of Endogenous Hydrogen Sulfide Synthesis Inhibits Mitochondrial Permeability Transition Pore Opening and Restores Constitutive No-Synthase Coupling in Old Rat Heart

Cited by 4 publications

References 17 publications

Quercetin Limits the Progression of Oxidative and Nitrosative Stress in the Rats’ Tissues After Experimental Traumatic Brain Injury

Quercetin Limits the Progression of Oxidative and Nitrosative Stress in the Rats’ Tissues After Experimental Traumatic Brain Injury

Garlic supplement lowers blood pressure in 40-60 years old hypertensive individuals, regulates oxidative stress, plasma cholesterol and protrombin index

Sex characteristics of hydrogen sulfide influence on ischemiareperfsion in miocardium of rats

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