Activated T Cells in the Surface Secretion on the Adenoid – a Flow Cytometric Study

Ivarsson, Magnus; Lundin, Björn; Lundberg, Christer

doi:10.1046/j.1365-3083.2002.01134.x

Cited by 6 publications

(8 citation statements)

References 17 publications

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“…Both B-and T-cell-related immunologic processes are initiated in different specialized compartments of the palatine tonsils (32,33). Also, the adenoid is an active immunologic site in the nasopharyngeal region that harbors cells from both the innate and adaptive immune systems (34,35).…”

Section: Discussionmentioning

confidence: 99%

Maternal Microchimerism in Juvenile Tonsils and Adenoids

et al. 2010

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During pregnancy small amounts of cells pass between the mother and the fetus, and this transfer may give rise to a chimeric state that persist for years in both individuals. Both fetal and maternal microchimerism (MMc) have been associated with different autoimmune disorders. Information about MMc in tissues of healthy individuals is sparse but is important when looking for maternal cells within affected tissues of certain diseases. The aim of this study was to investigate the occurrence of maternal cells in tonsils and adenoids of 20 healthy children between the ages of 2 and 15 years. All the children underwent surgery because of recurrent tonsillitis or respiratory obstruction. MMc was detected using an RT-PCR assay based on differences in gene polymorphisms between mother and child. We found maternal cells in the tonsils and/or adenoids in four of 20 children. This frequency is less than the frequency of maternal cells found in the peripheral blood of healthy adults but in agreement with the previously reported frequency of maternal chimerism in control tissues. (Pediatr Res 68: 199-204, 2010)

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Section: Discussionmentioning

confidence: 99%

Maternal Microchimerism in Juvenile Tonsils and Adenoids

et al. 2010

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“…The method of cell preparation, staining and assessment with flow cytometry was performed as described below, similarly to other authors [15][16][17].…”

Section: Methodsmentioning

confidence: 99%

Dendritic cells and lymphocyte subpopulations of the adenoid in the pathogenesis of otitis media with effusion

Kotowski

Niedzielski

Niedzielska

et al. 2011

International Journal of Pediatric Otorhinolaryngology

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“…A successful interaction between CD28/CTLA4 on T cells and B7-1 or B7-2 on antigen-presenting cells is necessary for T cell activation [1,2,3]. The balance of CD28- and CTLA4-derived signals profoundly alters the outcome of T cell activation; CTLA4 plays a critical role in the downregulation of T cell responses and may play an important role in the pathogenesis of the increased IgE response [4,5,6].…”

Section: Introductionmentioning

confidence: 99%

Expression of Fas Ligand and CTLA4 in Adenoids Has a Predictive Value for Allergic Rhinitis Development in Children

Zakrzewska

Gryczyńska²,

Kobos³

et al. 2006

Int Arch Allergy Immunol

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Background: The balance of CD28/CTLA4-derived signals and Fas-dependent apoptosis activity is determined by the peripheral defense mechanisms and might play a role in the pathogenesis of allergy. Objectives: The aim of the study was to investigate the expression of costimulatory and pro- and antiapoptotic molecules in adenoid T cells of children suffering from allergic rhinitis and to find out which of these molecules have a predictive value in the development of allergic rhinitis. Methods: The adenoids of 60 children, removed because of nasal obstruction, chronic rhinitis and recurrent respiratory infection, were evaluated. Patients were divided into two groups: group 1, suffering from chronic allergic rhinitis, and group 2, suffering from chronic rhinitis, where no specific IgE was detected, including children with a positive family history of allergy (group 2a) and children with neither a personal nor a family history of allergy (group 2b). For immunohistochemical stainings anti-CD3, anti-CD19, anti-CD4, anti-CD8, anti-CD25, anti-CD28, anti-CTLA4 (CD152), anti-bcl-2, anti-Fas, and anti-FasL antibodies were used. The number of cells expressing these molecules was identified in adenoid interfollicular spaces. The results were then analyzed in allergic and nonallergic children. During a 24-month follow-up children were re-examined for allergy and results were compared to previous immunohistochemical evaluations. Results: The expression of CD4, CD25, CD28, FasL, and CTLA4 was significantly increased in group 1 compared to group 2 (p < 0.05). However, the discriminant analysis confirmed that only CTLA4 and FasL expression fully discriminated allergic subjects from the others. During a 24-month period of observation 8 children from group 2a were also diagnosed with allergic rhinitis. All of them, especially those sensitized to mites, had an increased number of FasL+ and CTLA4+ in previously removed adenoids. Conclusion: An increased number of cells with intracellular expression of FasL and CTLA4, in interfollicular spaces of adenoids, seems to be a predictive factor of the development of allergic rhinitis.

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Activated T Cells in the Surface Secretion on the Adenoid – a Flow Cytometric Study

Cited by 6 publications

References 17 publications

Maternal Microchimerism in Juvenile Tonsils and Adenoids

Maternal Microchimerism in Juvenile Tonsils and Adenoids

Dendritic cells and lymphocyte subpopulations of the adenoid in the pathogenesis of otitis media with effusion

Expression of Fas Ligand and CTLA4 in Adenoids Has a Predictive Value for Allergic Rhinitis Development in Children

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