Action potential in a plant cell lowers the light requirement for non-photochemical energy-dependent quenching of chlorophyll fluorescence

Krupenina, Natalia A.; Булычев, А. А.

doi:10.1016/j.bbabio.2007.01.004

Cited by 86 publications

(75 citation statements)

References 40 publications

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“…For example, ESs induce gene expression, [16][17][18] phytohormone synthesis, 17,19,20 phloem transport decrease, 21 changes in root absorption, 11 activation of respiration, [22][23][24][25][26] and inactivation of photosynthesis. 23,24,[26][27][28][29][30][31] Retivin et al 32 hypothesized that the ultimate role of these physiological changes is the increase of plant resistance to stressors. Indeed, it has been shown that ESs can have a positive influence on resistance to stressors at the whole plant level, 32 including increasing photosynthetic machinery resistance, 30,33 which is strongly connected with inactivation of photosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Variation potential in higher plants: Mechanisms of generation and propagation

Vodeneev

Akinchits

Sukhov

2015

Plant Signaling & Behavior

158

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Section: Introductionmentioning

confidence: 99%

Variation potential in higher plants: Mechanisms of generation and propagation

Vodeneev

Akinchits

Sukhov

2015

Plant Signaling & Behavior

158

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“…A number of publications have demonstrated the influence of electrical signals on photosynthesis (Fromm and Eschrich 1993;Fromm and Fei 1998;Koziolek et al 2003;Bulychev et al 2004;Lautner et al 2005;Kaiser and Grams 2006;Hlaváčková et al 2006;Fromm and Lautner 2007;Grams et al 2007Grams et al , 2009Krupenina and Bulychev 2007;Krupenina et al 2008;Sukhov et al 2008a, b;Pavlovič et al 2011); however, these results are contradictory. In particular, electrical signals can induce rapid (*minutes) reduction in the CO 2 assimilation rate (Koziolek et al 2003;Lautner et al 2005;Grams et al 2009), a decrease in quantum yields of photosystem I (PSI) (Grams et al 2009) and photosystem II (PSII) (Koziolek et al 2003;Lautner et al 2005;Grams et al 2009), an increase in photochemical fluorescence quenching and a lowering in non-photochemical quenching (Pavlovič et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the photosynthetic response induced by variation potential in geranium

Sukhov

Orlova

Mysyagin

et al. 2011

Planta

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Electrical signals (action and variation potentials) caused by environmental stimuli induce a number of physiological responses in plants including changes in photosynthesis; however, mechanisms of these changes remain unclear. We investigated the influence of the variation potential on photosynthesis in geranium (Pelargonium zonale) under different conditions (control, low external CO₂ concentration, and actinic light absence). The variation potential caused by lamina burning induced a reduction in photosynthesis (decreases in effective quantum yields of photosystem I and II, CO₂ assimilation rate, and stomatal conductance) in unstimulated leaves under control conditions. Changes in the majority of light-stage parameters (photosystem I and II quantum yields, coefficients of photochemical and non-photochemical quenching, quantum yield of non-photochemical energy dissipation in photosystem I due to donor-side limitation) were correlated with a decrease in CO₂ assimilation rate. The changes were similar to those caused by lowering [CO₂]; their magnitudes decreased both under low external CO₂ concentration and without actinic light. These results support the hypothesis that Calvin cycle inactivation plays a key role in photosynthetic response induced by electrical signals. However, a decrease in electron transport through the PSI acceptor side also induced by variation potential was not correlated with a decrease in the CO₂ assimilation rate and did not depend on the external CO₂ concentration or actinic light intensity. Thus, we suggest that there are two different mechanisms of light-stage inactivation induced by the variation potential in geranium: one strongly dependent on dark-stage inactivation and one weakly dependent on dark-stage inactivation.

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“…The lower panel shows that the increase in R m was also associated with a pronounced decline in maximal chlorophyll fluorescence F m ' (curve 4), like it was observed in unconstrained cells. 20 This strong suppression of F m ' fluorescence, together with the decrease in actual fluorescence F t and the effective quantum yield of photosystem II primary reaction, ΔF/F m ' = (F m ' -F t )/F m ' (data not shown), suggest that the R m increase after AP was concurrent with changes in cytoplasmic composition that elevated thermal losses of In experiments shown in Figure 5, we monitored R m changes in the "alkaline" cell region during and after AP generation under continuous light (curve 1) and following a 30-min dark adaptation (curve 2). The dark period of such length is sufficient for almost complete flattening of the pH banding profile.…”

Section: Resultsmentioning

confidence: 99%

“…The saturation pulse method and its application to chlorophyll fluorescence measurements on microscopic cell regions (100 μm in diameter) were described previously. 20 During fluorescence measurements the actinic light was attenuated with neutral density glass filters.…”

Section: Methodsmentioning

confidence: 99%

Transient removal of alkaline zones after excitation ofCharacells is associated with inactivation of high conductance in the plasmalemma

Булычев

Krupenina

2009

Plant Signaling & Behavior

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The action potential (AP) of excitable plant cells is a multifunctional physiological signal. Its generation in characean algae suppresses the pH banding for 15-30 min and enhances the heterogeneity of spatial distribution of photosynthetic activity. This suppression is largely due to the cessation of H + influx (OH -efflux) in the alkaline cell regions. Measurements of local pH and membrane conductance in individual space-clamped alkaline zones (small cell areas bathed in an isolated pool of external medium) showed that the AP generation is followed by the transient disappearance of alkaline zone in parallel with a large decrease in membrane conductance. These changes, specific to alkaline zones, were only observed under continuous illumination following a relaxation period of at least 15 min after previous excitation. The excitation of dark-adapted cells produced no conductance changes in the post-excitation period. The results indicate that the origin of alkaline zones in characean cells is not due to operation of electroneutral H + /HCO 3 -symport or OH -/ HCO 3 -antiport. It is concluded that the membrane excitation is associated with inactivation of plasmalemma high conductance in the alkaline cell regions. IntroductionThe action potential (AP) of excitable plant cells is a physiological signal involved in regulation of osmotic balance, gene expression and other cell functions. 1 It also affects the plasmamembrane proton transport that plays a crucial role in regulation of cytoplasmic pH, electrogenesis, mineral nutrition and cell turgor of plant cells. 2 The electrochemical gradient Δμ H + created by the plasma membrane H + pump provides the driving force for accumulation of mineral nutrients, such as K + and NO 3 -, and benefits to photosynthesis of aquatic plants inhabiting weakly alkaline stagnant waters. [3][4][5] Local acidification of the apoplast near the cell surface to pH 6.3-6.7 increases the content of a neutral species CO 2 , which readily passes through the membranes unlike poorly permeable charged species HCO 3 -and CO 3 2-. In characean algae, which are close relatives of higher plants, the zones of H + extrusion (acid zones) alternate with spatially separated alkaline regions. 6,7 The alternated pattern of broad acidic and narrow alkaline zones, known as the pH banding phenomenon, is mirrored by the spatial pattern of photosynthetic activity in a single layer of immobile chloroplasts. 8 The pH banding pattern vanishes in darkness and is smoothed transiently after the propagation of action potential along the cell, 9 while the photosynthetic pattern becomes temporarily enhanced. 8 The long-lasting impact of AP on H + transport (pH banding) and photosynthesis (chlorophyll fluorescence) in characean algae is consistent with its possible regulatory role in these processes. The AP propagation inhibits pH banding and selectively suppresses photosynthesis in alkaline cell areas for the period of 15-30 min, while the signal itself (AP) lasts typically few seconds. It is not yet known whether the s...

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Action potential in a plant cell lowers the light requirement for non-photochemical energy-dependent quenching of chlorophyll fluorescence

Cited by 86 publications

References 40 publications

Variation potential in higher plants: Mechanisms of generation and propagation

Variation potential in higher plants: Mechanisms of generation and propagation

Analysis of the photosynthetic response induced by variation potential in geranium

Transient removal of alkaline zones after excitation ofCharacells is associated with inactivation of high conductance in the plasmalemma

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