This study deals with effects of membrane excitation on photosynthesis and cell protection against excessive light, manifested in non-photochemical quenching (NPQ). In Chara corallina cells, NPQ and pericellular pH displayed coordinated spatial patterns along the length of the cell. The NPQ values were lower in H(+)-extruding cell regions (external pH approximately 6.5) than in high pH regions (pH approximately 9.5). Generation of an action potential by applying a pulse of electric current caused NPQ to increase within 30-60 s. This effect, manifested as a long-lived drop of maximum chlorophyll fluorescence (F(m)'), occurred at lower photosynthetic flux densities (PFD) in the alkaline as compared to acidic cell regions. The light response curve of NPQ shifted, after generation of an action potential, towards lower PFD. The release of NPQ by nigericin and the rapid reversal of action potential-triggered NPQ in darkness indicate its relation to thylakoid DeltapH. Generation of an action potential shortly after darkening converted the chloroplasts into a latent state with the F(m) identical to that of unexcited cells. This state transformed to the quenched state after turning on weak light that was insufficient for NPQ prior to membrane excitation of the cells. The ionophore, A23187, shifted NPQ plots similarly to the action potential effect, consistent with a likely role of a rise in the cytosolic Ca(2+) level in the action potential-induced quenching. The results suggest that a rapid electric signal, across the plasma membrane, might exert long-lived effects on photosynthesis and chlorophyll fluorescence through ion flux-mediated pathways.
Even though ion/atom-collision is a mature field of atomic physics great discrepancies between experiment and theoretical calculations are still common. Here we present experimental results with highest momentum resolution on single ionization of helium induced by 1 MeV protons and compare these to different theoretical calculations. The overall agreement is strikingly good and already the first Born approximation yields good agreement between theory and experiment. This has been expected since several decades, but so far has not been accomplished. The influence of projectile coherence effects on the measured data is shortly discussed in line with an ongoing dispute on the existence of nodal structures in the electron angular emission distributions.
Comparison of two sets of structural and thickness maps of the Black Sea Basin produced by Russian and Italian workers revealed important differences in the interpretation of thickness and structure of the lower sedimentary unit, referred to in both works as ‘Palaeocene-Eocene’. The map based on the Italian data shows two depocentres with τ 5 km of sediment in the westernmost part of the Western Black Sea Basin (WBSB), while in the rest of the WBSB and in the Eastern Black Sea Basin (EBSB) the thickness is 2–3 km. Analysis of the land and submarine geology suggests that depocentres correspond to two segments of this system is represented by the Karkinit Graben on the northern shelf of the Black Sea. Submarine studies reveal that the graben originated behind and Early Cretaceous volcanic arc situated on the present day continental slope and rise.Most of the WBSB and the EBSB opened in the Eocene. For the EBSB this age is supported by data on its landward extension — the Adjaro—Trialet Basin. The EBSB could not open due to anticlockwise rotation of the Shatsky Rise because there was no corresponding subduction or shortening in the Greater Caucasus Basin. An alternative hypothesis is that of simultaneous opening of the EBSB and the WBSB as a result of southward drift of the Pontides and clockwise rotation of the Andrusov Rise.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.