Action of influenza virus neuraminidase on gangliosides

Slepushkin, Vladimir; Bukrinskaya, A. G.; Zhigis, L. S.; Reshetov, P. D.; Shaposhnikova, J.I.; Bergel'son, L. D.

doi:10.1016/0014-5793(85)80314-8

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…The substrate specificity of the various microbial sialidases varies, depending on the nature of substrate and the type of linkage between NANA and the neighbouring carbohydrate [1,15]. Thus, BACH & BRASHLER [5] have shown both similarities and differences in the action of various sialidases on rat mast cells.…”

Section: Discussionmentioning

confidence: 98%

Enhancement of histamine release from human basophils pretreated with different sialidases

et al. 1986

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Histamine release from human basophils was investigated in vitro after removal of cell membrane sialic acid by three different sialidases. Pretreatment of the cells with sialidases from Cl. Perfringens, V. Cholera or Influenza virus A2 enhanced histamine release induced by subsequent stimulation of the cells with anti-IgE or the plant lectin Concanavalin A and caused a shift to the left of the dose-response curve for anti-IgE. The enhanced histamine release was reflected in a increased calcium sensitivity, thus suggesting that cell membrane sialic acid might be involved in the calcium fluxes preceeding histamine release. In higher doses the sialidase from Cl. Perfringens caused the cells to release histamine by itself, whereas the sialidases from V. Cholera and Influenza virus A2 in high doses inhibited the cell response to Concanavalin A.

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Section: Discussionmentioning

confidence: 98%

Enhancement of histamine release from human basophils pretreated with different sialidases

et al. 1986

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“…Quantitative determination of ganglioside components was carried out as in [21]. The ganglioside mixtures (10-20 nmol sialic acid) were separated by HPTLC as described above.…”

Section: Analytical Proceduresmentioning

confidence: 99%

The gangliosides of adult human aorta: intima, media and plaque

Prokazova

Orekhov

Mukhin

et al. 1987

European Journal of Biochemistry

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The composition, structure and localization of gangliosides of aorta taken from subjects who had died after myocardial infarction were studied. Individual gangliosides were purified by high-performance liquid chromatography and high-performance thin-layer chromatography and were characterized on the basis of their chromatographic mobility, carbohydrate composition, neuraminidase hydrolysis and methylation analysis. The main aortic gangliosides were identified as GM3, GMI, GD3, GDla and GTlb. Significant differences in the ganglioside composition of intima and media were detected and the ganglioside profile of atherosclerotic plaques was found to differ markedly from that of unaffected intima. The latter was characterized by high content of GD3, a ganglioside thought to be associated with membrane permeability, cell interaction, adhesiveness and growth and to supress unspecific immune responses. Possible implications of the results in low-density lipoprotein binding to the arterial wall and in immunological changes induced by atherosclerotic lesions are discussed.Recently we found that several antigens reacting with monoclonal antibodies raised against aortic cell subpopulations are glycosphingolipids [2]. Earlier it has been shown that there is a threefold increase of gangliosides in the aorta during atherosclerosis [3]. Since gangliosides are important factors determining cell adhesiveness [4] and low-density lipoprotein binding [5] (and the literature cited therein), which both have been suggested to be involved in the development of atherosclerotic lesions (see for example [6]), the ganglioside composition of and localization in human aorta is of considerable interest. Nonetheless, information on this subject is sparse [3, 7, 81. With exeption of GM3 aortic gangliosides were identified only on the basis of their chromatographic mobility and no attempts to differentiate between intimal and medial glycolipids were undertaken. Here we present the results of a detailed study of the gangliosides of adult human aorta, its uninvolved intima, atherosclerotic plaques and underlying media. MATERIALS AND METHODS TissueThe human thoratic aortas from 40 -60-year-old men and women who had died of myocardial infarction were obtained at autopsy within 1.5 -3 h after sudden death. Atherosclerotic lesions (mainly atherosclerotic plaques) occupied, as a rule, about 50% of the total inner surface of the aorta. Adventitia together with one third of the outer media were stripped away and the remaining parts of aortic material were washed and frozen until analysis. The grossly normal areas and the unCorrespondence to N. V. Prokazova, Institut Cardiologii Akademii Meditsinskikh Nauk SSSR, 3 Cherepkovskaya ulitsa 15A, Moskva, USSR 121552Abbreviations. The designation of gangliosides follows the IUPAC-IUB recommendations [l]: GM3, I13NeuAcLacCer; Ghll, I13NeuAcGgOse4Cer; GD3, I13(NeuAc)2LacCer; GD1,, I13,1V3-(Ne~Ac)~GgOse~Cer; GTl I13(Ne~A~)ZIV3NeuAcGgOse4Cer. complicated atherosclerotic plaques were cut out and inner intima consistin...

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“…Our previous studies demonstrated that ganglioside GTl is involved in the reception of influenza virus and mediates entry of the virus into Ehrlich ascites carcinoma cells [4]. The virus was shown to convert G T l b mainly into ganglioside GDlb [5] the carbohydrate chain of which appears to be the minimal cell-surface structure capable of inducing influenza virus penetration into carcinoma cells. However, the mechanism by which gangliosides influence virus attachment and penetration remains obscure.…”

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confidence: 99%

Interaction of influenza virus with gangliosides and liposomes containing gangliosides

Slepushkin

Starov

Bukrinskaya

et al. 1988

European Journal of Biochemistry

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It has already been shown that influenza virus binds unspecifically to liposomes containing ganglioside GM1 whereas with gangliosides G D l b and G,,, binding occurs in a specific and saturable manner [Slepushkin et al. (1986) Biol. Membr. 3,229 -2351. In the present study the mode of interaction between influenza virus and various gangliosides or phospholipid liposomes containing cholesterol and gangliosides has been investigated.The influence of exogenous gangliosides on the structure of the viral envelope was studied using fluorescent and photoactivatable phospholipids incorporated into the viral membrane. With both types of probes maximal effects of gangliosides were caused by GT1b. Addition of that ganglioside resulted in a marked decrease in the fluorescence polarization (P) of fluorescent labeled virus as well as in substantial changes in the binding of photoactivatable analogues of sphingomyelin and phosphatidylcholine to virus proteins, mainly hemagglutinin.The effects of GTlb and GDlb on P value were comparable, whereas gangliosides with other oligosaccharide chains caused much smaller changes in P. Furthermore GTlb but not Gllll influenced phospholipid-hemagglutinin crosslinking.Interaction of the virus with large unilamellar liposomes was monitored by two fluorescence assays based on resonance-energy transfer from the tryptophans and tyrosines of viral proteins to vesicles labeled with a triacylglycerol (anthrylvinyldioleoyIglycero1) or from these labeled vesicles to virions labeled with a perylenoyl derivative of galactosylcerebroside (PGalSph). A third fluorescence assay was based on relief of self-quenching in PGalSph-labeled virions, upon low-pH-induced virus-liposome fusion. With all three fusion assays the changes of fluorescence caused by GTlb were more pronounced than those induced by GM1. On the other hand, virus-

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Action of influenza virus neuraminidase on gangliosides

Cited by 5 publications

References 12 publications

Enhancement of histamine release from human basophils pretreated with different sialidases

Enhancement of histamine release from human basophils pretreated with different sialidases

The gangliosides of adult human aorta: intima, media and plaque

Interaction of influenza virus with gangliosides and liposomes containing gangliosides

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