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Potential problems with the use of viral vectors for gene also enhanced by association with transferrin, GALA or the therapy necessitate the development of efficient nonviral influenza hemagglutinin N terminal peptide HA-2, but to a vectors. The association of transferrin, or the pH-sensitive smaller extent compared with the negatively charged compeptide GALA, with cationic liposomes composed of 1,2-plexes. The enhancement of gene delivery by transferrin dioleoyl-3-(trimethylammonium) propane and its equimolar or GALA was not affected significantly by the presence of mixture with dioleoylphosphatidylethanolamine, under conserum and did not cause significant cytotoxicity. Our ditions where the liposome/DNA complex is negatively results indicate that negatively charged ternary complexes charged, drastically increased luciferase expression from of cationic liposomes, DNA and transferrin, or fusigenic pCMVluc. The percentage of cells transfected, measured peptides, can facilitate efficient transfection of cultured by ␤-galactosidase expression, was also increased by cells, and that they may alleviate the drawbacks of the use about 10-fold. The zeta potential of the ternary complexes of highly positively charged complexes for gene delivery was lower than that of the liposome/DNA complexes.in vivo. Transfection activity of positively charged complexes was

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