Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals

Valera, Márcia Carneiro; Oliveira, Sarah Almeida Coelho de; Maekawa, Lilian Eiko; Cardoso, Flávia G.R.; Chung, Adriana; Silva, Stephanie F.P.; Carvalho, Cláudio Antônio Talge

doi:10.5005/jp-journals-10024-1812

Cited by 11 publications

(7 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diverse plant extracts were used in dentistry principally as endodontic irrigant or intracanal medication (Horiba, Maekawa, Ito, Matsumoto, & Nakamura, 1991;Maekawa et al, 2015;Qunqiang & Farnham, 1989;Marcia C Valera et al, 2016) or even associated with other intracanal medication (Marcia Carneiro Valera et al, 2015) and this may be related to the fact of the increased resistance of micro-organisms against antibiotics overtime (Jungermann et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Phytotherapy was used in dentistry and more specifically in endodontics since the last century (Groppo et al, 2008) due to its antimicrobial, anti-inflammatory and antifungal actions and because it is considered biocompatible (Guandalini Cunha et al, 2020;De La Chapa, Singha, Lee, & Gonzales, 2018;Marcia C Valera et al, 2016;Viegas et al, 2020) in addition to other techniques like photodynamic therapy and passive ultrasonic irrigation (Abu Hasna, Ferrari, & Talge Carvalho, 2019;Abu Hasna, Khoury, et al, 2020;Abu Hasna, Pereira Da Silva, et al, 2020). This use was encouraged by the World Health Organization (WHO); however, the agency establishes specific safety requirements including traditional use certificate and toxicity analyzes of the extracts (Organization, 2019).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts

Ferreira

Gonçalves

Pereira

et al. 2021

RSD

View full text Add to dashboard Cite

Objective: Achyrocline satureioides “A.satureioides” is a subshrub, widely distributed in South America because of its medicinal properties. Although it is widely used in folk medicine, there is still no approval for its therapeutic use, and its biocompatibility is little explored. This study aimed to evaluate its cytotoxicity and genotoxicity over human gingival fibroblasts (FMM-1). Methodology: Ten different concentration of the glycolic extract of A.satureioides were tested for 5min and 24h of contact with the cells to evaluate its cytotoxicity using the MTT colorimetric assay and to evaluate its genotoxicity using the micronucleus assay. Data were analyzed with one-way ANOVA and Tukey test with a significance level (α=0.05). Results: All tested concentrations of the extract presented cell viability more than 70% and has no significant difference of the control group after 5min and 24h. However, for 5min the 100 mg/mL was cytotoxic and for 24h the 1.56 mg/mL stimulated cell proliferation. For genotoxicity analysis, only the concentration 6.25 mg/mL showed results similar to the control of cell culture in the micronucleus count after 5min and 24h. Conclusions: The glycolic extract of A.satureioides doesn’t have cytotoxic and genotoxic effects in concentrations up to 6.25 mg/mL, but in high concentrations it is considered genotoxic.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts

Ferreira

Gonçalves

Pereira

et al. 2021

RSD

View full text Add to dashboard Cite

show abstract

“…In a study by Valera et al (2016), it was suggested that Z. officinale Roscoe may be clinically applicable as a root canal irrigant as 20% Z. officinale glycolic extracts were effective in reducing the endotoxins and microorganisms implicated in endodontic infections such as Candida albicans and Enterococcus faecalis.…”

Section: Zingiber Officinale Roscoe (Ginger)mentioning

confidence: 99%

The Effect of Zingiber officinale Roscoe (Ginger) on Dentin Microhardness: An in vitro Study

Zaki¹,

Baharin²,

Zaman³

et al. 2017

JAS

View full text Add to dashboard Cite

It has been established that conventional root canal irrigants cause detrimental effect on the physico-chemical properties of root dentin. This study aims to determine the effect of a novel root irrigant, ginger or Zingiber officinale Roscoe essential oil on root dentin microhardness. Eighty root halves of extracted human teeth were used and prepared by embedding them individually in autopolymerizing acrylic resin. These samples were then divided into four groups (n = 20 each), i.e., i) 0.5% Z. officinale Roscoe oil, ii) 2.5% sodium hypochlorite (NaOCl, positive control), iii) 17% ethylenediaminetetraacetic acid (EDTA, positive control) and iv) normal saline (negative control). Dentin microhardness was measured using Vickers Microhardness Tester with a 50 g load and a 10-second dwell time, before and after immersion in respective solutions for 5 minutes. Measurements were taken in Vickers hardness number (VHN). All samples showed reduction in the microhardness reading; with Z. officinale Roscoe: 34.24 to 25.89, NaOCl: 31.47 to 26.34, EDTA: 33.08 to 26.04 and saline: 29.82 to 27.61 VHN. Although there was significant reduction within the Z. officinale Roscoe group (p < 0.001), the change was indifferent when compared among groups (post hoc Tukey's HSD test p > 0.05). Within the limitations of this study, Zingiber officinale Roscoe essential oil does alter dentin microhardness but to a similar extent as NaOCl and EDTA.

show abstract

“…can be associated with chronic periodontal diseases and endodontic infections [6,7].The presence of enterococci within the root canal has been associated with persistent endodontic and periapical infections, being E. faecalis the most prevalent specie [8][9][10][11]. E. faecalis play an important role in post-treatment diseases because this specie can show high resistance to conventional antimicrobial agents, such as sodium hypochlorite, chlorhexidine, and calcium hydroxide [12][13][14]. In addition, certain clinical isolates of E. faecalis recovered from root canal infections demonstrated resistant to several antibiotics recommended for dental procedures, such as benzylpenicillin, ampicillin, clindamycin, and tetracycline [15], cephalosporins [8], azithromycin, and erythromycin [12].…”

Section: Introductionmentioning

confidence: 99%

Enterococcus faecium and Enterococcus faecalis in endodontic infections: antibiotic resistance profile and susceptibility to photodynamic therapy

et al. 2017

View full text Add to dashboard Cite

Introduction Enterococcus faecium has become an important microorganism in nosocomial infections with great ability to acquire antibiotic resistance. However, little is known about their presence on the oral cavity. Therefore, our objective was to verify the presence of E. faecium and E. faecalis in endodontic infections and compare their susceptibility to conventional antibiotics and photodynamic therapy. Methods We performed 38 collections from the root canals of different patients. Positive Enterococcus agar samples were submitted to phenotypic and genotypic testing for speciesspecific confirmation. The isolates identified as E. faecium and E. faecalis were tested for susceptibility to antibiotics by the E-test method. After that, the isolates were evaluated for susceptibility to photodynamic therapy (PDT) using methylene blue and gallium arsenide aluminum laser with a wavelength of 660 nm and fluence of 39.5 J/cm 2 (energy of 15 J and time of 428 s).Results Cultures positive for E. faecalis were found in 22 patients (58%). Among these patients, only two had E. faecium in mixed infections with E. faecalis. In the isolates of E. faecalis, 27% were resistant to antibiotics, including tetracycline, ciprofloxacin, and azithromycin. The isolates of E. faecium showed no resistance to the antibiotics tested. Both the isolates of E. faecium and E. faecalis exhibit significant susceptibility to PDT, including the isolates resistant to antibiotics. The reductions achieved by PDT ranged of 2.76 to 4.31 log 10 for E. faecalis strains and of 3.93 to 4.33 log 10 for E. faecium strains. Conclusion E. faecium showed lower prevalence in endodontic infections and higher susceptibility to antibiotics when compared to E. faecalis. In in vitro assays, PDT had a significant antimicrobial activity for both strains.

show abstract

Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals

Cited by 11 publications

References 29 publications

The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts

The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts

The Effect of Zingiber officinale Roscoe (Ginger) on Dentin Microhardness: An in vitro Study

Enterococcus faecium and Enterococcus faecalis in endodontic infections: antibiotic resistance profile and susceptibility to photodynamic therapy

Contact Info

Product

Resources

About