The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts

Abstract

Objective: Achyrocline satureioides “A.satureioides” is a subshrub, widely distributed in South America because of its medicinal properties. Although it is widely used in folk medicine, there is still no approval for its therapeutic use, and its biocompatibility is little explored. This study aimed to evaluate its cytotoxicity and genotoxicity over human gingival fibroblasts (FMM-1). Methodology: Ten different concentration of the glycolic extract of A.satureioides were tested for 5min and 24h of contact with the cells to evaluate its cytotoxicity using the MTT colorimetric assay and to evaluate its genotoxicity using the micronucleus assay. Data were analyzed with one-way ANOVA and Tukey test with a significance level (α=0.05). Results: All tested concentrations of the extract presented cell viability more than 70% and has no significant difference of the control group after 5min and 24h. However, for 5min the 100 mg/mL was cytotoxic and for 24h the 1.56 mg/mL stimulated cell proliferation. For genotoxicity analysis, only the concentration 6.25 mg/mL showed results similar to the control of cell culture in the micronucleus count after 5min and 24h. Conclusions: The glycolic extract of A.satureioides doesn’t have cytotoxic and genotoxic effects in concentrations up to 6.25 mg/mL, but in high concentrations it is considered genotoxic.

The Effect of Aloe vera and Chlorhexidine as Disinfectants on the Success of Selective Caries Removal Technique: A Randomized Controlled Trial

“…Phytotherapy is gaining a greater space in dentistry because of the antimicrobial effect, anti-inflammatory action, and biocompatibility of diverse herbal medicines [ 11 , 16 , 18 ]. Aloe vera extract as an herbal medicine was tested in this study to evaluate its effect on the success of the selective caries removal technique of deep carious lesions combined with asymptomatic reversible pulpitis when used as a cavity disinfectant.…”

Curcuma longa L. Extract and Photodynamic Therapy are Effective against Candida spp. and Do Not Show Toxicity In Vivo

“…Antimicrobial or antifungal photodynamic therapy (aPDT) consists of the combination of a photosensitizer with a light source that will produce reactive oxygen species [14] causing a cytotoxic effect on cancerous or microbial cells [15]. Some herbal medicines were tested and found effective over resistant microorganisms [16,17] beside being biocompatible [18]. Turmeric, Curcuma longa (C. longa), is a flowering plant of the ginger family, Zingiberaceae [19].…”

Antimicrobial and Antibiofilm Effect of Brazilian Green Propolis Aqueous Extract against Dental Anaerobic Bacteria

“…Phytotherapy is making great efforts in this aspect, in which diverse medicinal plants were presented recently as alternative antimicrobials to treat diverse infections because of their antimicrobial action and biocompatibility [ 16 , 17 , 18 , 19 ]. Propolis is a resinous substance enriched by biologically active molecules and is produced by Apis mellifera through the collection of different plant parts [ 20 ].…”