Action mechanisms of petroleum hydrocarbons present in waters impacted by an oil spill on the genetic material of Allium cepa root cells

Leme, Daniela Morais; Angelis, Dejanira de Franceschi de; Marin-Morales, Maria Aparecida

doi:10.1016/j.aquatox.2008.04.012

Cited by 106 publications

(48 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among the genotoxic effects observed, chromosome stickiness, which was observed more significantly after recovery treatment, is considered a result of aneugenic effect (LEME; ANGELIS; MARIN-MORALES, 2008). The most expressive changes, however, were nuclear buds.…”

Section: Discussionmentioning

confidence: 99%

“…In the concentrations tested here, chromosomal breakages happened in low amounts and presented no significant values. Chromosomal breakages and bridges are considered because of clastogenic effect (LEME; ANGELIS; MARIN-MORALES, 2008). Similarly to the breakages, chromosomal bridges were few expressive, being observed in significant amount only in the lowest tested concentration for both bioassays, so that for this parameter, it was not characterized the clastogenic action.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of allowed parameters for nickel in freshwater bodies using the Allium cepa test

Francisco

Christofoletti

Fontanetti

2014

Semin. Cienc. Biol. Saude

View full text Add to dashboard Cite

Compostos de níquel são descartados em grandes quantidades no ambiente, fazendo dele um contaminante de relevância ecotoxicológica, uma vez que podem se bioacumular ao longo da cadeia trófica e/ou induzir danos genéticos aos organismos expostos. Nesse sentido, a legislação brasileira estabelece o limite máximo da concentração total de níquel permitida em corpos de água doce em 0,025 mg/l, que consiste em um quarto do estabelecido pela Agência de Proteção Ambiental dos Estados Unidos (EPA). O presente trabalho avaliou três concentrações de níquel, na forma de cloreto de níquel (NiCl2), tendo como referência àquela estabelecida como limite pela resolução 357/2005 do CONAMA (National Environmental Council), utilizando sementes de Allium cepa, com o intuito de mensurar o potencial tóxico, citotóxico, genotóxico e mutagênico deste elemento. Em tais concentrações, não foi verificada toxicidade ou citotoxicidade, ao passo que, para as três concentrações empregadas, observou-se a indução de efeitos genotóxicos e potencialmente mutagênicos, principalmente de células portadoras de micronúcleos, brotos e aderências cromossômicas. Dessa forma, infere-se que o cloreto de níquel possui ação aneugênica e/ou clastogênica. Esse estudo também serve de alerta para a contaminação aquática por metais, visto que a menor concentração utilizada e a permitida pela legislação brasileira induziram danos no organismo testado. Sugere-se a necessidade de revisar os parâmetros estabelecidos para este metal em corpos de água doce. Palavras-chave: Mutagênese ambiental. Metais pesados. Genotoxicidade. Mutagenicidade. AbstractNickel compounds are disposed in large amounts in the environment, making it an ecotoxicological relevant contaminant, since these can bioaccumulate along the food chain and/or can cause DNA damage to the exposed organisms. In this sense, Brazilian law establishes 0.025 mg/l as the utmost boundary for the total nickel concentration allowed in freshwater bodies that consists in a quarter of the established by United States Environmental Protection Agency (EPA). This study evaluated three nickel concentrations, in nickel chloride (NiCl2) shape, having as reference the limit established by CONAMA (National Environmental Council), using seeds of Allium cepa, in order to measure the cytotoxic, genotoxic and mutagenic potentials of this element. At such concentrations, it was not observed toxicity or cytotoxicity, whereas for all three concentrations employed, there was induction of genotoxic and potentially mutagenic effects, especially cells bearing micronuclei, buds and chromosome stickiness. Thus, it is inferred that nickel chloride has aneugenic and/or clastogenic action. This study is also an alert for the water contamination by metals, since both concentration used, the lowest one and the one permitted by Brazilian law induced damage to the tested organism. The study suggests the necessity of review in the established parameters for such metal in freshwater bodies.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of allowed parameters for nickel in freshwater bodies using the Allium cepa test

Francisco

Christofoletti

Fontanetti

2014

Semin. Cienc. Biol. Saude

View full text Add to dashboard Cite

show abstract

“…25.04%, 27.53%, 33.18% and 39.48% of total aberrant cells were found at 25 %, 50 %, 75 % and 100 %, respectively during root dip treatment in PG sample. Many authors consider the plant bioassays as first alerts/biological indicators to explore the harmful consequences of environmental complex mixtures (Sujetoviene and Griauslyte, 2008;Leme et al, 2008;Hoshina and Marin-Morales, 2009). The clastogenic damages in terms of tumor induction were further observed in the second bioassay i.e.…”

Section: Resultsmentioning

confidence: 99%

Genotoxicity and tumor inducing potential of roadside soil samples exposed to heavy traffic emissions at Amritsar (Punjab), India

Kaur

Pakade

Katnoria

2013

JANS

View full text Add to dashboard Cite

Noxious gases released from vehicles adversely affect the microbial population of the roadside soil as well as exerts influence on plant growth and development. Considering the increasing vehicular pollution in Amritsar, a holy city of Punjab (India) and their serious consequences in terms of health problems, the present study was planned to estimate genotoxic and tumor inducing potentials of soil samples from Golden temple (GT) and Putlighar Chowk (PG) by employing Allium cepa root chromosomal aberration assay (AlRCAA) and Potato disc tumor assay (PDTA), respectively. The genotoxic potential in terms of percent aberrant cells in A. cepa was found to be 29.24 % (GT) and 29.24 % (PG) during in situ treatment and 39.00 % (GT) and 39.48 % (PG) during root dip treatment. The average number of tumors was found to be 14.4 in PG sample, followed by 13.0 in GT sample. Both samples have shown high content of lead viz., 39.81 (GT) and 56.48 (PG) during physicochemical analysis of the samples.

show abstract

“…Analyses of the aberration types showed clastogenic and aneugenic effects for the roots exposed to the polluted waters from Guaecá river, besides the induction of cell death. Probably all the observed effects were induced by the petroleum hydrocarbons derived from the oil leakage [39]. In the work of [40], the general toxicity (root growth inhibition and malformation) and genotoxicity (induction of chromosome aberrations in root cells) of an oil field wastewater in the Nigeria have been investigated by the Allium test.…”

Section: Allium Cepa Test 'In Focus'mentioning

confidence: 99%

Bioassays with Plants in the Monitoring of Water Quality

Barbério¹

2013

Water Treatment

View full text Add to dashboard Cite

Action mechanisms of petroleum hydrocarbons present in waters impacted by an oil spill on the genetic material of Allium cepa root cells

Cited by 106 publications

References 26 publications

Evaluation of allowed parameters for nickel in freshwater bodies using the Allium cepa test

Evaluation of allowed parameters for nickel in freshwater bodies using the Allium cepa test

Genotoxicity and tumor inducing potential of roadside soil samples exposed to heavy traffic emissions at Amritsar (Punjab), India

Bioassays with Plants in the Monitoring of Water Quality

Contact Info

Product

Resources

About