Bioassays with Plants in the Monitoring of Water Quality

Barbério, Agnes

doi:10.5772/50546

Cited by 10 publications

(9 citation statements)

References 48 publications

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“…A. cepa can detect several classes of contaminants, such as heavy metals, domestic and industrial sewage, landfill extracts and water samples from rivers and lakes, whose solutions include a complex mixture of substances of different compositions. It is efficient and confirms sensitivity and effectiveness (Barbério, 2013).…”

Section: Introductionmentioning

confidence: 56%

Evaluation of the cytotoxic and genotoxic effect of Allium cepa L. (Amaryllidaceae) root cells after exposure in water samples of five lakes of Alta Floresta, State of Mato Grosso

et al. 2020

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This study evaluated genotoxic and cytotoxic potential of water samples through the analysis of Allium cepa root cells exposed to samples from five urban lakes of Alta Floresta, state of Mato Grosso. The samples were obtained in the dry and rainy periods, October 2014 and April 2015, respectively, at five distinct points. The collection points were as follows: Lake 01, located in MT 206; Lake 02, in Avenida Perimetral Rogério Silva; Lake 03, in Sector C; Lake 04 on Teles Pires Avenue; and Lake 05, in the Cidade Bela District. Bioassays using Allium cepa bulbs were taken from the water at each point, and comparisons were made with the negative and positive controls. The results found the presence of genotoxic and cytotoxic activities on the roots of A. cepa, indicating high potential in cell cycle inhibition. This result may also be caused by the influences of seasonal periods, taking into account that in the rainy season there is a greater transport of cytotoxic substances by rainwater, and in the dry season, a higher concentration of pollutants due to the reduction of water volume, resulting in a greater interaction of the concentrators as well as a greater concentration of the elements found in the water of these lagoons.

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Section: Introductionmentioning

confidence: 56%

Evaluation of the cytotoxic and genotoxic effect of Allium cepa L. (Amaryllidaceae) root cells after exposure in water samples of five lakes of Alta Floresta, State of Mato Grosso

et al. 2020

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“…This test-object allows recording various types of chromosomal mutations induced both by mutagens directly damaging DNA and promutagenes acquiring genetic activity in the living organism in the process of metabolism (Fiskesio, 1985;Seth et al, 2008;Leme & Marin-Morales, 2009;Yildiz et al, 2009;Geras'kin et al, 2011b;Barberrio, 2013;Trushin et al, 2013;Firbas & Amon, 2014;Dutta & Ahmad, 2016;Silveira et al, 2016;Cabuga et al, 2017;Duarte et al, 2017). It has been established that the results obtained by using A. cepa are highly correlated with those carried out on mammalian cells, including human ones (Ghosh et al, 2011).…”

Section: Introductionmentioning

confidence: 85%

“…Higher plants are recognized as excellent genetic models to detect environmental mutagens, and are, therefore, frequently used in monitoring studies (Kovalchuk et al, 2001;Shevtsova & Gudkov, 2009;Geras'kin et al, 2011a;Goncharova & Lyashenko, 2011;Sadowska et al, 2011;Khlebova & Ereschenko, 2012;Barberrio, 2013;Kalaev & Popova, 2014;Shevtsova et al, 2014;Alexeyeva et al, 2016;Geras'kin et al, 2016;Medvedeva & Bolsunovsky, 2016;Prysedskyj, 2016).…”

Section: Introductionmentioning

confidence: 99%

Toxic and mutagenic activities of surface water from the Chumysh River

2017

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The Allium test system is worldwide used for biological monitoring of environmental pollutants. We have studied toxic and mutagenic activity of water from the Chumysh River, which is one of the largest tributaries of the Upper Ob. Water samples were taken near industrial settlement Talmenka (Altai Territory of Russia) during the spring flood and low autumn water level in 2015. Root tips of Allium cepa L. was used as a biological model. There were examined 30 samples. Purified tap water of medium hardness served as a control sample. After the synchronizing the cell divisions, the onion bulbs with roots reaching a length of 2-3 mm were transferred to glass cups containing the selected samples of River water and cultured for several days at a temperature of +24 ± 1°C. After exposure, root tips were rinsed in distilled water and fixed in a cold mixture of ethanol and acetic acid (3:1). Fixed samples were used after 12-24 h or transferred to 70% alcohol and stored in refrigerator at a temperature of +4°C until required. The fixed materials were hydrolyzed in 1N HCl at 60°C for 5-8 min and squashed in aceto-orcein. Prepared slides were viewed under the microscope at a magnification of ×90. The mitotic index (MI), the phase indices, the frequency of abnormal mitosis, and chromosomal aberrations were determined by the examination of 500 cells per a replicate (100 cells per slide). We established that the decreased or increased levels of mitotic activity and the frequencies of pathological mitoses (up to 7.9%, P < 0.05) in onion root tips revealed the presence of mitotoxic and genotoxic agents in the Chumysh River water. We found that the most number of chromosomal abnormalities occurs at the stages of meta-or anaphase. The main abnormalities are chromosome laggings in meta-and anaphase, chromosome bridges, chromosome fragments and micronuclei. Their number increased in 5.0-10.8 times compared with the control value. It has been discovered the temporal and spatial distribution of compounds with different toxicity and genotoxicity within a stream. The greatest level of mitotic depression and the highest frequency of chromosomal mutations were observed in the tissues of the onion root tips germinated on the samples collected in low autumn water. The mechanisms of plant adaptation to unfavorable environmental factors are discussed.

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“…Its use was recommended as early as in the 1970's by the Royal Swedish Academy of Science and later by the Gene-Tox program [21]. Cytogenotoxicity screening of polluted water using the A. cepa bioassay serve as perfect monitoring systems for the detection of environmental chemicals that may pose genetic hazards in living organisms.…”

Section: Introductionmentioning

confidence: 99%

Toxicological Evaluation of Drinking Water Sources in Some Rural Communities in Southern Nigeria after Mycofiltration Treatment

Olorunfemi¹,

Efechuku²,

Esuana³

2015

Pol. J. Environ. Stud.

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Water is essential for human existence, and its importance for individual health as well as the well-being of a nation cannot be overemphasized. Fresh water represents the main sources of safe water for household use, sustainable development, and human survival. They comprise water from lakes, hand-dug wells, taps, boreholes, streams, rivers and their plains, wetlands, and those available in underground reservoirs, making them unavoidable for the evolution of society and civilization [1]. In spite of the considerable investments of the Nigerian government in water supply programs, over 52% of its citizens have no access to potable water [2]. The fresh water sources available to the local inhabitants are either unsafe or difficult to obtain. In some instances women and children need to walk for hours to fetch ordinary drinking water. An estimated 140 million people are continuously drinking water containing all kinds of germs, heavy metals, bacteria, and dust particles capable of causing various diseases [3].

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Bioassays with Plants in the Monitoring of Water Quality

Cited by 10 publications

References 48 publications

Evaluation of the cytotoxic and genotoxic effect of Allium cepa L. (Amaryllidaceae) root cells after exposure in water samples of five lakes of Alta Floresta, State of Mato Grosso

Evaluation of the cytotoxic and genotoxic effect of Allium cepa L. (Amaryllidaceae) root cells after exposure in water samples of five lakes of Alta Floresta, State of Mato Grosso

Toxic and mutagenic activities of surface water from the Chumysh River

Toxicological Evaluation of Drinking Water Sources in Some Rural Communities in Southern Nigeria after Mycofiltration Treatment

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