Actinoplanes abujensis sp. nov., isolated from Nigerian arid soil

Sazak, Anil; Şahi̇n, Nevzat; Camas, Mustafa

doi:10.1099/ijs.0.031872-0

Cited by 13 publications

(7 citation statements)

References 36 publications

(33 reference statements)

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“…Due to the close phylogenetic relationships among known species and between many of our samples and those known species, the final taxonomic placements of most of our isolates, especially the yellow-highlighted ones, such as S3-21 and S3-31, in Figure 6, await further investigation using either multilocus sequence typing or whole-genome sequencing. Soil is a dominant niche of actinobacteria, especially for genera Sacchrothrix and Actinoplanes [105][106][107][108][109][110]. A previous metagenomic analysis of endophytic bacteria of the xerophilous moss Grimmia montana revealed the bacterial community inside this moss was composed of Proteobacteria, Firmicutes, Actinobacteria, and Cytophaga/Flexibacter/Bacteroids [13].…”

Section: Discussionmentioning

confidence: 99%

Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand

et al. 2021

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Actinobacteria are a group of ecologically important bacteria capable of producing diverse bioactive compounds. However, much remains unknown about the taxonomic and metabolic diversities of actinobacteria from many geographic regions and ecological niches. In this study, we report the isolation of actinobacteria from moss and moss-associated rhizosphere soils in Thailand. Among the 89 isolates analyzed for their bioactivities, 86 strains produced indole-3-acetic acid (IAA, ranging from 0.04 to 59.12 mg/L); 42 strains produced hydroxamate type of siderophore; 35 strains produced catecholate type of siderophore; 21 strains solubilized tricalcium phosphate; and many strains exhibited antagonistic activities against one to several of the seven selected plant, animal, and human pathogens. Overall, actinobacteria from the rhizosphere soil of mosses showed greater abilities to produce IAA and siderophores and to solubilize tricalcium phosphate than those from mosses. Among these 89 isolates, 37 were analyzed for their 16S rRNA gene sequences, which revealed their diverse phylogenetic distributions among seven genera, Streptomyces, Micromonospora, Nocardia, Actinoplanes, Saccharothrix, Streptosporangium, and Cryptosporangium. Furthermore, gas chromatography-mass spectrometry analyses of ethyl acetate crude extracts of three selected isolates with inhibitory effects against a methicillin-resistant Staphylococcus aureus strain revealed diverse metabolites with known antimicrobial activities. Together, our results demonstrate that actinobacteria from mosses in Thailand are taxonomically diverse and capable of producing a range of metabolites with plant-growth-promoting and microbial pathogen-inhibiting potentials.

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Section: Discussionmentioning

confidence: 99%

Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand

et al. 2021

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“…Phosphatidylethanolamine is the diagnostic phospholipid. The G+C contents of the genomic DNA vary from 67 to 76 % (Sazak et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Actinoplanes aureus sp. nov., a novel protease-producing actinobacterium isolated from soil

Song

Sun

Luo

et al. 2021

Antonie van Leeuwenhoek

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A novel protease-producing actinobacterium, designated strain NEAU-A11 T , was isolated from soil collected from Aohan banner, Chifeng, Inner Mongolia Autonomous Region, China, and characterised using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-A11 T was indicated to belong to the genus Actinoplanes and was most closely related to Actinoplanes rectilineatus JCM 3194 T (98.9 %). Cell walls contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were arabinose, xylose and glucose. The phospholipid pro le contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and two phosphatidylinositol mannosides. The predominant menaquinones were MK-9(H 4 ), MK-9(H 6 ) and MK-9(H 8 ). The major fatty acids were C 18:0 , C 16:0 , C 18:1 ω9c, C 17:0 and C 15:0 . Genome sequencing revealed a genome size of 10,742,096 bp, a G + C content of 70.5 % and 9,514 protein-coding genes (CDSs), including 102 genes coding for protease. Genome mining analysis using antiSMASH 5.0 led to the identi cation of 20 putative gene clusters responsible for the production of diverse secondary metabolites. Phylogenetic analysis using the 16S rRNA gene sequences showed that the strain formed a stable clade with A. rectilineatus JCM 3194 T in the genus Actinoplanes. However, whole-genome average nucleotide identity (ANI) value and the levels of digital DNA-DNA (dDDH) hybridization between strain NEAU-A11 T and A. rectilineatus JCM 3194 T was 81.1 % and 24.6 % (22.2-27.0 %), respectively. The values were well below the criteria for species delineation of 70% for dDDH and 95-96% for ANI, suggesting that the isolate differed genetically from its closely related type strain. In addition, evidences from phenotypic, chemotaxonomic and genotypic studies indicate that strain NEAU-A11 T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes aureus sp. nov. is proposed, with NEAU-A11 T (= CCTCC AA 2019063 T = JCM 33971 T ) as the type strain.

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“…Predominant cellular fatty acids are often present as iso-/anteiso-branched and monounsaturated components and/or cis -9,10-octadecanoic acid (Tamura & Hatano, 2001). The DNA G+C content is 67–76 mol% (Sazak et al , 2012).…”

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Actinoplanes luteus sp. nov., isolated from soil

Suriyachadkun

Ngaemthao

Chunhametha

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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A novel filamentous bacterial strain, A-T 5190 T , which developed irregular sporangia at the end of sporangiophores on substrate mycelia, was isolated from dry evergreen forest soil collected in Thailand. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 5190 T belonged to the genus Actinoplanes and was related most closely to Actinoplanes palleronii NBRC 14916 T (98.88 % similarity) and Actinoplanes rectilineatus NBRC 13941 T (98.54 %). DNA-DNA relatedness values between strain A-T 5190 T and its closest relatives were below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The wholecell sugars contained rhamnose, ribose, galactose and xylose. The predominant menaquinone was MK-9(H 4 ). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were unsaturated fatty acid C 17 : 1 and branched fatty acids iso-C 16 : 0 , iso-C 15 : 0 and anteiso-C 17 : 0 . The G+C content of the genomic DNA was 71.9 mol%. Evidence from phenotypic, chemotaxonomic and genotypic studies indicate that strain A-T 5190 T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes luteus sp. nov. is proposed. The type strain is A-T 5190 T (5BCC 41582 T 5NBRC 109644 T ).

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Actinoplanes abujensis sp. nov., isolated from Nigerian arid soil

Abstract: A novel actinobacterial strain, A4029 T , isolated from arid soil of Abuja, Nigeria, and provisionally assigned to the genus Actinoplanes, was subjected to a polyphasic taxonomic study. 16S rRNA gene sequence similarity studies showed that strain A4029T belonged to the genus Actinoplanes,

Cited by 13 publications

References 36 publications

Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand

Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand

Actinoplanes aureus sp. nov., a novel protease-producing actinobacterium isolated from soil

Actinoplanes luteus sp. nov., isolated from soil

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