Actinoplanes aureus sp. nov., a novel protease-producing actinobacterium isolated from soil

Abstract

A novel protease-producing actinobacterium, designated strain NEAU-A11 T , was isolated from soil collected from Aohan banner, Chifeng, Inner Mongolia Autonomous Region, China, and characterised using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-A11 T was indicated to belong to the genus Actinoplanes and was most closely related to Actinoplanes rectilineatus JCM 3194 T (98.9 %). Cell walls contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were arabinose, xylose and glucose. The phospholipid pro le contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and two phosphatidylinositol mannosides. The predominant menaquinones were MK-9(H 4 ), MK-9(H 6 ) and MK-9(H 8 ). The major fatty acids were C 18:0 , C 16:0 , C 18:1 ω9c, C 17:0 and C 15:0 . Genome sequencing revealed a genome size of 10,742,096 bp, a G + C content of 70.5 % and 9,514 protein-coding genes (CDSs), including 102 genes coding for protease. Genome mining analysis using antiSMASH 5.0 led to the identi cation of 20 putative gene clusters responsible for the production of diverse secondary metabolites. Phylogenetic analysis using the 16S rRNA gene sequences showed that the strain formed a stable clade with A. rectilineatus JCM 3194 T in the genus Actinoplanes. However, whole-genome average nucleotide identity (ANI) value and the levels of digital DNA-DNA (dDDH) hybridization between strain NEAU-A11 T and A. rectilineatus JCM 3194 T was 81.1 % and 24.6 % (22.2-27.0 %), respectively. The values were well below the criteria for species delineation of 70% for dDDH and 95-96% for ANI, suggesting that the isolate differed genetically from its closely related type strain. In addition, evidences from phenotypic, chemotaxonomic and genotypic studies indicate that strain NEAU-A11 T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes aureus sp. nov. is proposed, with NEAU-A11 T (= CCTCC AA 2019063 T = JCM 33971 T ) as the type strain.

Phylogenomic analyses of the genus Actinoplanes: description of four novel genera

“…Members of the genus Actinoplanes are important elements of biotechnological research. Some members of the genus can degrade natural and systemic rubbers [13], flavonoids and their glycosides [14], and can produce several lytic enzymes [15]. Bacteria of this genus are widely used in pharmacology because they produce bioactive compounds.…”

Actinoplanes maris sp. nov., isolated from marine sediment

“…The characteristic menaquinone of the genus is MK-9(H 4 ), and phosphatidylethanolamine is the diagnostic phospholipid. At the time of writing, the genus comprised 53 species with validly published names (www.bacterio.net/actinoplanes.html) and two non-validly named species ‘ Actinoplanes solisilvae ’ [5] and ‘ Actinoplanes aureus ’ [6].…”