Actinomycetes of Loktak Habitat: Isolation and Screening for Antimicrobial Activities

Singh, Laishram Shantikumar; Baruah, Indra; Bora, Tarun C.

doi:10.3923/biotech.2006.217.221

Cited by 50 publications

(19 citation statements)

References 16 publications

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“…Actinobacteria are prokaryotes, having high guanine and cytosine (GC) content in their DNA, with highly variable metabolic possibilities. The metabolic diversity of the Actinobacteria class is due to their extremely large genome, which has hundreds of transcription factors that control gene expression, allowing them to respond to specific needs (Shantikumar et al, 2006). The class Actinobacteria comprises five subclasses, 10 orders, 56 families and 286 genera (Ait Barka et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial potential of actinomycetes isolated from unexplored hot Merzouga desert and their taxonomic diversity

et al. 2018

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Summary StatementMerzouga sand dunes were among the Moroccan extreme environments. This ecosystem is relatively unexplored and present habitat to the search for Actinobacteria with potential antimicrobial activities and biotechnological applications. Biology Open • Accepted manuscriptby guest on April 27, 2019 http://bio.biologists.org/ Downloaded from Abstract:The absence of new antibiotics is guiding more and more researchers to specific ecosystems.One hundred sixty-three Actinobacteria isolates were isolated from Merzouga sand and

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Section: Introductionmentioning

confidence: 99%

Antimicrobial potential of actinomycetes isolated from unexplored hot Merzouga desert and their taxonomic diversity

et al. 2018

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“…Of the actinomycetes, streptomycetes are particularly prolific antibiotic producers, which grow as a branched multicellular network of hyphae, called the mycelium (Claessen et al , 2014); on solid surfaces they reproduce through spores formed by a specialized aerial mycelium, which coincides with antibiotic production (Hopwood, 2007). Many studies have been conducted in the search for novel actinomycetes (Lazzarini et al , 2000; Ng & Amsaveni, 2012; Singh et al , 2006; Takahashi & Omura, 2003; Thakur et al , 2007). However, the intensive screening programs in the pharmaceutical industry failed to yield significant numbers of novel drugs (Payne et al , 2007).…”

Section: Introductionmentioning

confidence: 99%

Eliciting antibiotics active against the ESKAPE pathogens in a collection of actinomycetes isolated from mountain soils

et al. 2014

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The rapid emergence of multidrug-resistant (MDR) bacterial pathogens poses a major threat for human health. In recent years, genome sequencing has unveiled many poorly expressed antibiotic clusters in actinomycetes. Here, we report a well-defined ecological collection of >800 actinomycetes obtained from sites in the Himalaya and Qinling mountains, and we used these in a concept study to see how efficiently antibiotics can be elicited against MDR pathogens isolated recently from the clinic. Using 40 different growth conditions, 96 actinomycetes were identified – predominantly Streptomyces – that produced antibiotics with efficacy against the MDR clinical isolates referred to as ESKAPE pathogens: Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and/or Enterobacter cloacae. Antimicrobial activities that fluctuated strongly with growth conditions were correlated with specific compounds, including borrelidin, resistomycin, carbomethoxy-phenazine, and 6,7,8- and 5,6,8-trimethoxy-3-methylisocoumarin, of which the latter was not described previously. Our work provided insights into the potential of actinomycetes as producers of drugs with efficacy against clinical isolates that have emerged recently and also underlined the importance of targeting a specific pathogen.

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“…Actinobacteria are the most prolific producer of antibiotics[ 5 ]. Out of more than eight thousand antimicrobial products described in antibiotic literature database (ABL database), 45.6% are produced by Streptomycetes and 16% by strains belonging to rare genera of actinobacteria[ 3 ], which forms an important component of the soil microflora[ 6 ]. The rate of discovery of new compounds from terrestrial actinobacteria has decreased, whereas the rate of reisolation of known compounds has increased[ 7 ].…”

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confidence: 99%

Antimicrobial screening of actinobacteria using a modified cross-streak method

Velho-Pereira

Kamat

2011

Indian J Pharm Sci

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Out of the 30 actinobacterial cultures screened for antimicrobial activity, 28 cultures were found to produce active products against various pathogenic microorganisms such as Gram-negative, Gram-positive bacteria and yeast, using a modified cross streak method. The modified method helped in easy quantification of results and also in ruling out probable mutual antibiosis. The actinobacterial strains that showed the ability to produce antimicrobial compounds belonged to Streptomyces (53%), Micromonospora (13%) and Actinomadura (10%) genera. Streptomyces sp. strain MMA-5 showed the highest multispecific antibiosis efficiency score value. Broad antibiotic spectrum activity was exhibited by Streptomyces sp. strain MMA-2 and Micromonospora sp. strain MMA-8. The multidrug resistant human pathogenic yeast strain Candida albicans was inhibited by 18 actinobacterial strains.

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Actinomycetes of Loktak Habitat: Isolation and Screening for Antimicrobial Activities

Cited by 50 publications

References 16 publications

Antimicrobial potential of actinomycetes isolated from unexplored hot Merzouga desert and their taxonomic diversity

Antimicrobial potential of actinomycetes isolated from unexplored hot Merzouga desert and their taxonomic diversity

Eliciting antibiotics active against the ESKAPE pathogens in a collection of actinomycetes isolated from mountain soils

Antimicrobial screening of actinobacteria using a modified cross-streak method

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