Actin-binding proteins undergo major alterations during the plasma membrane transformation in uterine epithelial cells

Terry, Vera; Shaw, Timothy; Shorey, C. D.; Murphy, Christopher R.

doi:10.1002/(sici)1097-0185(199609)246:1<71::aid-ar8>3.0.co;2-i

Cited by 19 publications

(6 citation statements)

References 31 publications

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“…Vinculin, a major component of focal adhesions and cell-cell junctions [31], can directly alter cell motility [32]. We propose that the altered abundance of these actinbinding proteins in the porcine endometrium is important during the implantation process as has been reported in other mammals [33,34]. Actin and its binding proteins might have a role in the stabilization of the cytoskeleton of endometrial tissue undergoing morphological changes.…”

Section: Discussionmentioning

confidence: 76%

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Jalali

Bogacki

Dietrich

et al. 2015

Journal of Proteomics

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Section: Discussionmentioning

confidence: 76%

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Jalali

Bogacki

Dietrich

et al. 2015

Journal of Proteomics

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“…[36][37][38] Remodeling of the actin cytoskeleton during early rat pregnancy has been shown to involve the actin-binding proteins gelsolin, alpha-actinin, and vinculin. 39 This study has demonstrated that in healthy human endometrial epithelial cells, cofilin and slingshot change localization from the apical pole in the proliferative phase of the menstrual cycle to the basal pole in the secretory phase. Slingshot may migrate with cofilin in order to maintain an active cofilin pool, thereby promoting actin remodeling and uterine receptivity.…”

Section: Discussionmentioning

confidence: 86%

Cofilin and Slingshot Localization in the Epithelium of Uterine Endometrium Changes During the Menstrual Cycle and in Endometriosis

et al. 2011

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Regulation of the actin cytoskeleton is essential for epithelial cell polarity and protein trafficking within human uterine epithelium. The actin-binding protein cofilin is involved in regulation of actin dynamics by promoting actin branching and cytoskeleton reorganization. Dual immunohistochemical staining of cofilin and G-actin (represented by DNAse I staining) revealed cofilin-G-actin colocalization in the apical side of luminal epithelial cells of human uterine endometrium during the proliferative phase of the menstrual cycle. Interestingly, during the secretory phase of the menstrual cycle cofilin was only present on the basolateral side. To determine whether the disease endometriosis causes a different pattern of actin remodeling, we investigated an established baboon model of induced endometriosis. The cofilin pattern in the secretory phase of baboons with endometriosis was similar to the proliferative phase in normal animals; cofilin was observed in the apical parts of luminal and glandular epithelium. A phosphatase regulating the activity of cofilin, slingshot (SSH1), revealed a similar staining pattern within these tissues. These patterns were confirmed through quantitative image analysis. Quantification of mRNA detected upregulated SSH1 and suggested a progesterone resistance related pattern of nuclear steroid hormone receptors, but no change in membrane progesterone receptors (mPR alpha or mPR beta) was observed in endometriosis. Our data indicate that the severe dyssynchrony during menstrual cycle phases in endometriosis is connected with improper cytoskeleton rearrangements. We suggest that cofilin-mediated actin reorganization in uterine epithelial cells might be important in preparation for blastocyst implantation; dysregulation of this reorganization may lead to decreased fertility in endometriosis.

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“…During the plasma membrane transformation, a key part of this remodelling involves marked changes of the apical cytoskeleton, which consists of a filamentous actin terminal web that dissociates at the time of implantation (Luxford and Murphy 1992a). In addition, numerous actin and membrane-binding proteins play important roles in the restructuring of the apical domain of UECs in preparation for blastocyst implantation (Terry et al 1996). Adhesion molecules are also redistributed to the apical membrane in order to participate in uterine epithelium-embryo adhesion (Aplin 1997).…”

Section: Introductionmentioning

confidence: 99%

Ezrin and EBP50 redistribute apically in rat uterine epithelial cells at the time of implantation and in response to cell contact

2010

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Uterine epithelial cells (UECs) undergo extensive morphological remodelling in preparation for an implanting blastocyst. This remodelling involves changes in the actin cytoskeleton and surface structures including microvilli. Ezrin and ezrin-radixin-moesin-binding protein-50-kDa (EBP50) link actin filaments to intra-membranous adhesion molecules and are important molecules in polarised epithelia. The current study is the first to describe the colocalisation and molecular association of ezrin and EBP50 in rat UECs by using immunofluorescence microscopy and immunoprecipitation techniques. These proteins have also been localised in relation to uterine epithelial cytoskeletal rearrangement during early pregnancy in the rat and to the effect of apical surface contact between opposing epithelial cells, blastocyst contact and contact with a silicon filament. Immunofluorescence microscopy has revealed that ezrin and EBP50 respond to contact between opposing epithelial cells and increase apically on day 6 of pregnancy. This apical distribution is also observed in UECs in contact with a silicon filament. Ezrin and EBP50 are however absent within the implantation chamber itself, seemingly mimicking the events that take place in leucocyte-endothelium binding. Thus, ezrin and EBP50 occur apically in UECs at the time of implantation in the rat and in response to a substitute blastocyst (filament) suggesting a role for these proteins in the cytoskeletal rearrangements that facilitate uterine receptivity and blastocyst-epithelial adhesion. Their loss within the implantation chamber possibly allows the subsequent invasion of the embryo.

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Actin-binding proteins undergo major alterations during the plasma membrane transformation in uterine epithelial cells

Cited by 19 publications

References 31 publications

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Cofilin and Slingshot Localization in the Epithelium of Uterine Endometrium Changes During the Menstrual Cycle and in Endometriosis

Ezrin and EBP50 redistribute apically in rat uterine epithelial cells at the time of implantation and in response to cell contact

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