Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Jalali, Beenu Moza; Bogacki, Marek; Dietrich, Mariola A.; Likszo, Pawel; Wasielak, Marta

doi:10.1016/j.jprot.2015.05.003

Cited by 34 publications

(50 citation statements)

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“…6B). A Venn-diagram comparison of the present findings with the differential proteins expression from Days 9 to 12 of cycling and pregnant porcine endometria (Jalali et al, 2015) revealed 20 unique proteins whose abundance changes only between cycling and pregnant endometria ( Fig. 7), which together identify specific biological functions that may be regulated by the conceptus.…”

Section: Discussionmentioning

confidence: 70%

“…Uterine serpins were suggested to have evolved to act as iron carriers in pigs (Padua and Hansen, ). Interestingly, our previous results show that one spot, identified as alpha‐1‐antitrypsin, increased on Day 12, independent of the presence of a concceptus, whereas another protein spot, identified as serpin A3‐8, decreased as the cycling endometrium progressed from Days 9 to 12 (Jalali et al, ). The increase in abundance of serpin A3‐8 and serpin A3‐6 isoforms in the pregnant endometrium was conceptus driven.…”

Section: Discussionmentioning

confidence: 88%

“…A pregnancy‐induced increase in endometrial haptoglobin was previously reported in human (Berkova et al, ) and rabbit (Hoffman et al, ); its dysregulation causes fetal growth restriction in humans (Gupta et al, ). The expression of ceruloplasmin, a copper‐binding antioxidant protein, was previously observed to increase in the endometrium between Days 9 and 12, irrespective of the pregnancy status (Jalali et al, ). In the present study, we observed that additional isoforms of ceruloplasmin show differential abundance between Day 12 of cycling and pregnant endometria, with more protein associated with the latter state.…”

Section: Discussionmentioning

confidence: 93%

“…While the abundance of the proteins in this category can be altered by changes in ovarian steroid hormones alone, the expression of a few proteins seems to be modulated by conceptus‐secreted factors, including coflin, cyclophilins A and B, and PDIA3. The abundance of beta‐actin, and its binding proteins gelsolin and cofilin, changes between Days 9 and 12 in cycling endometria (Jalali et al, ). In particular, cofilin abundance decreases between cycling Days 9 and 12, whereas the presence of conceptuses maintained its abundance in the Day‐12 pregnant endometrium.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic and network analysis of pregnancy-induced changes in the porcine endometrium on Day 12 of gestation

2016

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Conceptus attachment is a time-sensitive process that requires a synchronized uterine environment created by molecular changes in the endometrium in response to ovarian hormones and conceptus signals. Porcine conceptuses undergo rapid elongation and differentiation, and secrete estrogens that serve as maternal-recognition-of-pregnancy signals during the peri-implantation period (Days 11-12). Pregnancy-induced proteomic changes in the porcine endometrium were measured during this period using two-dimensional differential gel electrophoresis of endometrial protein lysates from Day-12 pregnant versus non-pregnant animals (n = 4 each). Forty-four differentially abundant proteins in the pregnant endometrium were identified by mass spectrometry. The pregnant endometrium was associated with a unique protein profile, revealed by principal component analysis. A pregnancy-dependent increase in the abundance of serpins, cofilin, annexin A2, aldose reductase, cyclophilin, protein disulphide isomerase A3, and peroxiredoxin 1 was observed. Western blotting for some of the selected proteins confirmed their enrichment during pregnancy. Ingenuity pathway analysis identified several functions specifically over-represented among the differentially abundant proteins in the pregnant endometrium, including calcium signaling, angiogenesis, leukocyte migration, and cell movement. Interleukin-1 beta and beta-estradiol were identified as upstream regulators of several high-abundance proteins from pregnancy. Therefore, signals from porcine conceptuses, such as estrogens, interleukin 1B, and epidermal growth factor, either alone or in coordination with other factors, prepare the uterus for implantation. Mol. Reprod. Dev. 83: 827-841, 2016 © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Proteomic and network analysis of pregnancy-induced changes in the porcine endometrium on Day 12 of gestation

2016

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“…This increased early embryonic loss is generally attributed to impaired communication between the developing blastocyst and the maternal endometrium (Farin et al, 2010). In the past three decades, tremendous advances have been made in deciphering some of the MRP signaling particularly in ruminants (Sandra et al, 2015;Spencer et al, 2007;Spencer et al, 2008), swine (Bazer and Johnson, 2014;Franczak and Bogacki, 2009;Jalali et al, 2015) and to a lesser extent in horses (Katila, 2011;Klein and Troedsson, 2011a, b).…”

Section: Introductionmentioning

confidence: 99%