Acteoside protects against 6-OHDA-induced dopaminergic neuron damage via Nrf2-ARE signaling pathway

Li, Maiquan; Zhou, Fei; Xu, Tao; Song, Huaxin; Lu, Baiyi

doi:10.1016/j.fct.2018.06.018

Cited by 76 publications

(30 citation statements)

References 31 publications

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“…The results demonstrated that the bioavailability of formononetin and calycosin was reduced, the duration of formononetin was prolonged, and the absorption rate of calycosin increased. Acteoside is a primary active substance in the SNH extract and shows antioxidant and neuroprotective effects (Li, Zhou, Xu, Song, & Lu, 2018). In this study, acteoside could not be absorbed by rats in the SNH group, whereas its concentration increased in rat plasma of the QSKL group ( C max was 18.34 ng·mL −1 , AUC 0–48h was 34.93 ng/mL·h, and AUC 0–∞ was 38.10 ng/mL·h), indicating that the bioavailability was significantly enhanced after the herbal combination.…”

Section: Resultsmentioning

confidence: 99%

Quantitative analysis and pharmacokinetic comparison of multiple bioactive components in rat plasma after oral administration of Qi‐Shen‐Ke‐Li formula and its single‐herb extracts using ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Zhou

Zheng

et al. 2020

Biomedical Chromatography

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Qi‐Shen‐Ke‐Li (QSKL), a traditional Chinese formula prepared from six herbs, has long been used for the treatment of coronary heart disease and chronic heart failure. However, the herbal combination mechanism and underlying material basis of this multi‐herbal formula are not clear. In this study, an ultra‐high‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method to simultaneously determine multiple bioactive compounds in QSKL was established and validated. Using the developed method, 18 bioactive components in rat plasma after oral administration of QSKL formula and its single herb extracts were quantified. Based on these results, pharmacokinetic (PK) parameters (T1/2, Tmax, Cmax, AUC0–48h, and AUC0–∞) of the 18 bioactive components were analyzed and compared using PKSlover 2.0 PK software. The experimental data suggested that significant changes in PK profiles were observed between the QSKL formula and its single‐herb extracts. The herbal combination in QSKL significantly influences the system exposure and the PK behaviors of the 18 bioactive components, indicating multicomponent interactions among the herbs. This study provides insight into the herbal combination mechanism and underlying material basis of the QSKL formula.

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Section: Resultsmentioning

confidence: 99%

Quantitative analysis and pharmacokinetic comparison of multiple bioactive components in rat plasma after oral administration of Qi‐Shen‐Ke‐Li formula and its single‐herb extracts using ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Zhou

Zheng

et al. 2020

Biomedical Chromatography

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“…Thus, complex plant extracts containing multi-target agents that interact with their receptors in a pleiotropic fashion generate a pharmacological synergism that affects numerous processes, including the movement of polar metabolites across cell membranes. In this context, Li et al [26] employed a zebrafish model to demonstrate that acteoside could penetrate the blood-brain barrier, and proposed that the phenylethanoid glycoside may have a potential therapeutic effect in Parkinson's disease.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Phenylethanoid Glycosides from Aloysia polystachya and Its Activity as Inhibitors of Monoamine Oxidase-A

Pereira

Guimarães

Pereira

et al. 2019

PMIO

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Aloysia polystachya is used as a sedative and antidepressant by the indigenous populations of Argentina and Paraguay, but the compounds associated with these activities have not been determined. We have separated and identified the main constituents of the hydroethanolic extract of A. polystachya by ultra-performance liquid chromatography-mass spectrometry and confirmed the presence of acteoside, isoacteoside, 6'-acetylacteoside, and 4’,4’’’,5,5’’-tetrahydroxy-6,6’’,3’’’-trimethoxy-[C7–O–C7’’]-biflavone by NMR spectroscopy. Inhibitory activities of the hydroethanolic extract and purified phenylethanoid glycosides against monoamine oxidase-A were assessed using a standard fluorometric assay. The hydroethanolic extract inhibited monoamine oxidase-A activity in a dose-dependent manner with an IC50 of 9.2 µg/mL, while the selective monoamine oxidase inhibitor clorgyline exhibited an IC50 of 0.06 µg/mL (0.22 µM). Acteoside was the strongest inhibitor of monoamine oxidase-A (IC50 value of 5 µM), whereas isoacteoside and 6'-acetylacteoside showed IC50 values of around 10 µM. The results showed that phenylethanoids from a hydroethanolic extract of A. polystachya have been found to have inhibitory activity against monoamine oxidase-A. It is likely that the mode of action of the acteosides is multi-targeted, involving the downregulation of inflammatory molecules and neutralization of oxidation reactions as well as inhibition of monoamine oxidase-A.

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“…Under homeostatic conditions, Nrf2 influences mitochondrial membrane potential, fatty acid oxidation, the availability of substrates for respiration, as well as ATP synthesis [44]. But under stress conditions, Nrf2 detaches from Keap1 and translocates to the nucleus, and counteracts the promotion of ROS production through transcriptional increase of antioxidant proteins, including SOD, CAT, HO1, NQO1, GCLM and GCLC, which subsequently modulate oxidative stress, apoptosis and inflammation in diverse neurological disorders [45,46]. Previous studies have documented that after SCI, oxidative stress markers specific to lipid and protein oxidation, namely 4-HNE and 3-NT, all up-regulate in the injured tissue homogenates [47,48].…”

Section: Discussionmentioning

confidence: 99%

Targeting CARD6 attenuates spinal cord injury (SCI) in mice through inhibiting apoptosis, inflammation and oxidative stress associated ROS production

Wang

Luo

Liu

2019

Aging

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Spinal cord injury (SCI) causes long-term and severe disability, influencing the quality of life and triggering serious socioeconomic consequences. Lack of effective pharmacotherapies for SCI is largely attributable to an incomplete understanding of its pathogenesis. Caspase recruitment domain family member 6 (CARD6) was initially suggested to be a protein playing significant role in NF-κB activation. However, the effects of CARD6 on SCI progression remain unknown. In this study, the wild type (CARD6+/+), CARD6 knockout (CARD6-/-) and CARD6 transgenic (TG) mice were subjected to a SCI model in vivo, and in vitro experiments were conducted by treating microglia cells with lipopolysaccharide (LPS). Here, we identified CARD6 as a suppressor of SCI in mice. CARD6 knockout significantly accelerated functional deficits, neuron death and glia activation, whereas CARD6 overexpression resulted in the opposite effects. Both in vivo and in vitro SCI models suggested that CARD6 knockout markedly promoted apoptosis by increasing Cyto-c release to cytosol from mitochondria and activating Caspase-3 signaling. In addition, CARD6 knockout mice exhibited stronger inflammatory response after SCI, as evidenced by the significantly elevated expression of pro-inflammatory cytokines TNF-α, IL-1β and IL-6, which was largely through enhancing the activation of NF-κB signaling.

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Acteoside protects against 6-OHDA-induced dopaminergic neuron damage via Nrf2-ARE signaling pathway

Cited by 76 publications

References 31 publications

Quantitative analysis and pharmacokinetic comparison of multiple bioactive components in rat plasma after oral administration of Qi‐Shen‐Ke‐Li formula and its single‐herb extracts using ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Quantitative analysis and pharmacokinetic comparison of multiple bioactive components in rat plasma after oral administration of Qi‐Shen‐Ke‐Li formula and its single‐herb extracts using ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Isolation and Identification of Phenylethanoid Glycosides from Aloysia polystachya and Its Activity as Inhibitors of Monoamine Oxidase-A

Targeting CARD6 attenuates spinal cord injury (SCI) in mice through inhibiting apoptosis, inflammation and oxidative stress associated ROS production

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