Acquisition of oocyte competence to develop as an embryo: integrated nuclear and cytoplasmic events

Conti, Marco; Franciosi, Federica

doi:10.1093/humupd/dmx040

Cited by 234 publications

(180 citation statements)

References 268 publications

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“…The fully mature oocyte, created through the process of oocyte maturation, is at the very heart of the reproductive strategy because of its ability to develop into a totipotent zygote . Oocyte developmental competence is described as the ability of an oocyte to mature into an oocyte with the potential to be fertilized and to maintain embryo development until the blastocyst stage . Indeed, fertilization and embryo development are both deeply rooted in oocyte maturation.…”

Section: Introductionmentioning

confidence: 99%

“…1,2 Oocyte developmental competence is described as the ability of an oocyte to mature into an oocyte with the potential to be fertilized and to maintain embryo development until the blastocyst stage. 3 Indeed, fertilization and embryo development are both deeply rooted in oocyte maturation. Oocyte maturation is crucial for oocyte quality and for the attainment of haploidy, 2 and inappropriate or insufficient oocyte maturation can cause poor oocyte quality, fertilization failure, abnormal fertilization, and aneuploidy.…”

Section: Introductionmentioning

confidence: 99%

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Melatonin promotes human oocyte maturation and early embryo development by enhancing clathrin‐mediated endocytosis

Liu

et al. 2019

Journal of Pineal Research

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Embryo development potential and reproductive clinical outcomes are all deeply rooted in oocyte maturation. Melatonin has been reported to promote oocyte maturation as an antioxidant in nonprimate species. Its antioxidative functions also help reduce plasma membrane rigidity, which facilitates clathrin‐mediated endocytosis (CME). Whether melatonin has effects on human oocyte maturation by regulating CME is worthy of exploration. In this study, we found that the optimal melatonin concentration for human oocyte maturation was 10−11 M, and the maturation rate of this group was 71.9% (P = .03). The metaphase II (MII) stage oocytes obtained by in vitro maturation with 10−11 M melatonin had a significantly higher fertilization rate (81.4% vs 61.4%, respectively, P = .017) and blastocyst rate (32.2% vs 15.8%, respectively, P = .039) compared to controls. During maturation, antioxidative melatonin greatly enhanced CME and decreased intra‐oocyte cAMP level. The former was evidenced by the increasing numbers of coated pits and vesicles, and the upregulated expression of two major CME markers—clathrin and adaptor protein‐2 (AP2). CME inhibitor dynasore increased intra‐oocyte cAMP level and blocked oocyte maturation, and melatonin could partly rescue oocyte maturation and significantly elevate the expression of clathrin and AP2 in the presence of dynasore. Therefore, we conclude that melatonin could promote human oocyte maturation and early embryo development through enhancing CME.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Melatonin promotes human oocyte maturation and early embryo development by enhancing clathrin‐mediated endocytosis

Liu

et al. 2019

Journal of Pineal Research

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“…The late MI data are derived from the experiment described inFig. 1b, MII translation data are from a deposited dataset generated from oocytes matured in vivo followed by polysome fractionation/microarray (polysome array)27, 42 ; and prophase-to-MII total mRNA data were from a deposited dataset43 . The data are reported as scatterplots with LFC in total mRNA CPM at either late MI or MII compared to prophase I arrest versus the LFC of ribosome-bound mRNA CPM at the same time points.…”

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confidence: 99%

Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption

Luong

Daldello

Rajkovic³

et al. 2019

Preprint

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Cell development relies on elaborate changes in gene expression in order to transition 38 through different phenotypic and functional stages that ultimately lead to terminal differentiation. 39Changes in gene expression are achieved through transcriptional and post-transcriptional 40 regulations. Although transcriptional regulation is understood in considerable detail 1, 2 , much less 41 is known about the molecular machinery involved in translation regulation. 42Large oligomeric complexes involving proteins and non-coding RNAs are assembled on 43 the mRNA 3 to regulate its interaction with ribosomes, its translation rate, and its stability 4, 5 . In 44 somatic cells, numerous observations indicate that translation is intimately coupled with 45 degradation of mRNAs 5, 6 . Proteins recruited on the mRNA interact with elements located 46 throughout the length of the transcript 3, 7 . However, complexes nucleated around the 5' and 3' 47 polyadenylation element-binding protein (CPEB) is considered a master regulator of 63 polyadenylation and translation 18,19 . Much less is known about the role of CPEB in mammalian 64 oocytes. Here, we have used a genome-wide approach to investigate the role of this RNA-binding 65 protein (RBP) during the transition from quiescence to re-entry into the meiosis. Through a 66 detailed time course, we have investigated the temporal association between maternal mRNA 67 translation and the different steps involved in oocyte re-entry into and progression through the 68 meiosis. Using a RiboTag/RNA-Seq strategy, we describe a genome-wide switch in the 69 translation program of maternal mRNAs, and define new, critical functions of CPEB in the control 70 of this switch. 71 4 Results 72 Re-entry into meiosis coincides with rapid translational changes of stable mRNAs 73We have used a RiboTag/RNA-Seq strategy to characterize mRNA translation in oocytes 74 arrested at prophase I (GV) and in those undergoing meiotic maturation. (Fig. 1a). This strategy 75 has been previously validated 20, 21 and additional quality controls are reported (Supplementary 76 Fig. 1a-d). Upon meiotic resumption, there were both progressive increases and decreases in 77 ribosome loading of maternal mRNAs (Fig. 1b). By late metaphase I (MI), mRNAs were either 78 constitutively translated (n = 5580, CONSTITUTIVE), translationally repressed (n = 1092, 79 DOWN), or translationally activated (n = 871, UP) (FDR ≤ 0.05 and -1 ≥ log2(FC) (LFC) ≥ 1, 80 Supplementary Fig. 1e). Total mRNA levels remained stable up to MI and significant 81 destabilization was only detectable for 3% of maternal mRNAs at late MI ( Fig. 1b and 82 Supplementary Fig. 1f). Comparison of changes in total mRNA levels (transcriptome) to changes 83 in ribosome-associated mRNA levels (translatome) confirms this late MI destabilization (Fig. 1c), 84 which became prominent later during MII arrest, with a subset of mRNAs remaining stable even 85 if their translation was repressed. Changes in translation that initiate during MI were extended into 86 and amplified ...

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“…Oocyte maturation is the process whereby the oocyte accomplishes meiosis (GV → GV breakdown (GVBD) → metaphase I (MI) → metaphase II (MII)). Meiosis is regulated by numerous fertility factors, among which maturation-promoting factor (MPF, composed of cyclin B and cdk1), 1 spindle checkpoint proteins 2 Based on recent knowledge, researchers agree that oocyte maturation should include cytoplasmic, nuclear and epigenetic maturation, [6][7][8] and further studies are required to completely resolve the mechanism. Recently, researchers identified many potential female fertility factors through transcriptome [8][9][10][11] and proteome-wide [12][13][14] analyses.…”

Section: Introductionmentioning

confidence: 99%

The 5.8S pre‐rRNA maturation factor, M‐phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis

et al. 2020

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Objectives M‐phase phosphoprotein 6 (MPP6) is important for 5.8S pre‐rRNA maturation in somatic cells and was screened as a female fertility factor. However, whether MPP6 functions in oocyte meiosis and fertility is not yet known. We aimed to address this. Materials and Methods Mouse oocytes with surrounded nucleus (SN) or non‐surrounded nucleus (NSN) were used for all experiments. Peptide nanoparticle‐mediated antibody transfection was used to deplete MPP6. Immunofluorescence staining, immunohistochemistry and live tracker staining were used to examine MPP6 localization and characterize phenotypes after control or MPP6 depletion. High‐fidelity PCR and fluorescence in situ hybridization (FISH) were used to examine the localization and level of 5.8S rRNAs. Western blot was used to examine the protein level. MPP6‐EGFP mRNA microinjection was used to do the rescue. Results MPP6 was enriched within ovaries and oocytes. MPP6 depletion significantly impeded oocyte meiosis. MPP6 depletion increased 5.8S pre‐rRNA. The mRNA levels of MPP6 and 5.8S rRNA decreased within ageing oocytes, and MPP6 mRNA injection partially increased 5.8S rRNA maturation and improved oocyte quality. Conclusions MPP6 is required for 5.8S rRNA maturation, meiosis and quality control in mouse oocytes, and MPP6 level might be a marker for oocyte quality.

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Acquisition of oocyte competence to develop as an embryo: integrated nuclear and cytoplasmic events

Cited by 234 publications

References 268 publications

Melatonin promotes human oocyte maturation and early embryo development by enhancing clathrin‐mediated endocytosis

Melatonin promotes human oocyte maturation and early embryo development by enhancing clathrin‐mediated endocytosis

Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption

The 5.8S pre‐rRNA maturation factor, M‐phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis

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